A Synthetic Peptide from HIV-1 gp41 Is a Potent Inhibitor of Virus-Mediated Cell—Cell Fusion

1993 ◽  
Vol 9 (11) ◽  
pp. 1051-1053 ◽  
Author(s):  
Carl Wild ◽  
Teresa Greenwell ◽  
Thomas Matthews
Keyword(s):  
Cell Fusion ◽  
Synthetic Peptide ◽  
Potent Inhibitor ◽  
Hiv 1 ◽  
Cell Cell

scholarly journals Cell–cell fusion and internalization of the CNS-based, HIV-1 co-receptor, APJ

Virology ◽  
2003 ◽  
Vol 307 (1) ◽  
pp. 22-36 ◽  
Author(s):  
Naiming Zhou ◽  
Xuejun Fan ◽  
Muhammad Mukhtar ◽  
Jianhua Fang ◽  
Charvi A Patel ◽  
...  
Keyword(s):  
Cell Fusion ◽  
Hiv 1 ◽  
Cell Cell

scholarly journals Cell–cell and virus–cell fusion assay–based analyses of alanine insertion mutants in the distal α9 portion of the JRFL gp41 subunit from HIV-1

2019 ◽  
Vol 294 (14) ◽  
pp. 5677-5687 ◽  
Author(s):  
Mizuki Yamamoto ◽  
Qingling Du ◽  
Jiping Song ◽  
Hongyun Wang ◽  
Aya Watanabe ◽  
...  
Keyword(s):  
Cell Fusion ◽  
Hiv 1 ◽  
Insertion Mutants ◽  
Cell Cell

scholarly journals The Mechanism of Inhibition of HIV-1 Env-Mediated Cell–Cell Fusion by Recombinant Cores of gp41 Ectodomain

Virology ◽  
2002 ◽  
Vol 302 (1) ◽  
pp. 174-184 ◽  
Author(s):  
Ruben M. Markosyan ◽  
Xiuwen Ma ◽  
Min Lu ◽  
Fredric S. Cohen ◽  
Grigory B. Melikyan
Keyword(s):  
Cell Fusion ◽  
Gp41 Ectodomain ◽  
Mechanism Of Inhibition ◽  
Hiv 1 ◽  
Cell Cell

d(TGGGAG) with 5′-nucleobase-attached large hydrophobic groups as potent inhibitors for HIV-1 envelop proteins mediated cell–cell fusion

2011 ◽  
Vol 21 (19) ◽  
pp. 5762-5764 ◽  
Author(s):  
Wei Chen ◽  
Liang Xu ◽  
Lifeng Cai ◽  
Baohua Zheng ◽  
Kun Wang ◽  
...  
Keyword(s):  
Cell Fusion ◽  
Hiv 1 ◽  
Cell Cell

scholarly journals BROADLY NEUTRALIZING ANTI- HIV-1 ANTIBODIES DO NOT INHIBIT HIV-1 ENV-MEDIATED CELL-CELL FUSION

2021 ◽  
Author(s):  
Nejat Düzgüneş ◽  
Michael Yee ◽  
Deborah Chau
Keyword(s):  
Cell Fusion ◽  
Neutralizing Antibodies ◽  
Culture Media ◽  
Genetic Material ◽  
Inhibitory Effect ◽  
Hippeastrum Hybrid Agglutinin ◽  
Calcein Am ◽  
Antibodies Monoclonal ◽  
Hiv 1 ◽  
Cell Cell

PG9, PG16, PGT121, and PGT145 antibodies were identified from culture media of activated memory B-cells of an infected donor and shown to neutralize many HIV-1 strains. Since HIV-1 spreads via both free virions and cell-cell fusion, we examined the effect of the antibodies on HIV-1 Env-mediated cell-cell fusion. Clone69TRevEnv cells that express Env in the absence of tetracycline were labeled with Calcein-AM Green, and incubated with CD4+ SupT1 cells labeled with CellTrace Calcein Red-Orange, with or without antibodies. Monoclonal antibodies PG9, PG16, 2G12, PGT121, and PGT145 (at up to 50 μg/mL) had little or no inhibitory effect on fusion between HIV-Env and SupT1 cells. By contrast, Hippeastrum hybrid agglutinin completely inhibited fusion. Our results indicate that transmission of the virus or viral genetic material would not be inhibited by these broadly neutralizing antibodies. Thus, antibodies generated by HIV-1 vaccines should be screened for their inhibitory effect on Env-mediated cell-cell fusion.

scholarly journals Rational Site-Directed Mutations of the LLP-1 and LLP-2 Lentivirus Lytic Peptide Domains in the Intracytoplasmic Tail of Human Immunodeficiency Virus Type 1 gp41 Indicate Common Functions in Cell-Cell Fusion but Distinct Roles in Virion Envelope Incorporation

2003 ◽  
Vol 77 (6) ◽  
pp. 3634-3646 ◽  
Author(s):  
Vandana Kalia ◽  
Surojit Sarkar ◽  
Phalguni Gupta ◽  
Ronald C. Montelaro
Keyword(s):  
Human Immunodeficiency Virus ◽  
Cell Fusion ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Syncytium Formation ◽  
Immunodeficiency Virus ◽  
Arginine Residues ◽  
Hiv 1 ◽  
Cell Cell

ABSTRACT Two highly conserved cationic amphipathic α-helical motifs, designated lentivirus lytic peptides 1 and 2 (LLP-1 and LLP-2), have been characterized in the carboxyl terminus of the transmembrane (TM) envelope glycoprotein (Env) of lentiviruses . Although various properties have been attributed to these domains, their structural and functional significance is not clearly understood. To determine the specific contributions of the Env LLP domains to Env expression, processing, and incorporation and to viral replication and syncytium induction, site-directed LLP mutants of a primary dualtropic infectious human immunodeficiency virus type 1 (HIV-1) isolate (ME46) were examined. Substitutions were made for highly conserved arginine residues in either the LLP-1 or LLP-2 domain (MX1 or MX2, respectively) or in both domains (MX4). The HIV-1 mutants with altered LLP domains demonstrated distinct phenotypes. The LLP-1 mutants (MX1 and MX4) were replication defective and showed an average of 85% decrease in infectivity, which was associated with an evident decrease in gp41 incorporation into virions without a significant decrease in Env expression or processing in transfected 293T cells. In contrast, MX2 virus was replication competent and incorporated a full complement of Env into its virions, indicating a differential role for the LLP-1 domain in Env incorporation. Interestingly, the replication-competent MX2 virus was impaired in its ability to induce syncytia in T-cell lines. This defect in cell-cell fusion did not correlate with apparent defects in the levels of cell surface Env expression, oligomerization, or conformation. The lack of syncytium formation, however, correlated with a decrease of about 90% in MX2 Env fusogenicity compared to that of wild-type Env in quantitative luciferase-based cell-cell fusion assays. The LLP-1 mutant MX1 and MX4 Envs also exhibited an average of 80% decrease in fusogenicity. Altogether, these results demonstrate for the first time that the highly conserved LLP domains perform critical but distinct functions in Env incorporation and fusogenicity.

scholarly journals Estimating the Threshold Surface Density of Gp120-CCR5 Complexes Necessary for HIV-1 Envelope-Mediated Cell-Cell Fusion

PLoS ONE ◽  
2011 ◽  
Vol 6 (5) ◽  
pp. e19941 ◽  
Author(s):  
Shiva Naresh Mulampaka ◽  
Narendra M. Dixit
Keyword(s):  
Cell Fusion ◽  
Surface Density ◽  
Hiv 1 ◽  
Cell Cell
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