Functional Characterization of Type III-A CRISPR-Cas in a Clinical Human Methicillin-R Staphylococcus aureus Strain

2021 ◽  
Author(s):  
Yang Li ◽  
Kasper Mikkelsen ◽  
Oleguer Lluch i Grané ◽  
Zhenyu Wang ◽  
Yuanyue Tang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Functional Characterization ◽  
Aureus Strain ◽  
Type Iii

scholarly journals Functional Characterization of a Na+-Coupled Dicarboxylate Carrier Protein from Staphylococcus aureus

2005 ◽  
Vol 187 (15) ◽  
pp. 5189-5194 ◽  
Author(s):  
Jason A. Hall ◽  
Ana M. Pajor
Keyword(s):  
Staphylococcus Aureus ◽  
Structural Analysis ◽  
Transport Properties ◽  
Sequence Homology ◽  
Cytoplasmic Membrane ◽  
Functional Characterization ◽  
Carrier Protein ◽  
Dependent Manner ◽  
Reaction Sequence

ABSTRACT We have cloned and functionally characterized a Na+-coupled dicarboxylate transporter, SdcS, from Staphylococcus aureus. This carrier protein is a member of the divalent anion/Na+ symporter (DASS) family and shares significant sequence homology with the mammalian Na+/dicarboxylate cotransporters NaDC-1 and NaDC-3. Analysis of SdcS function indicates transport properties consistent with those of its eukaryotic counterparts. Thus, SdcS facilitates the transport of the dicarboxylates fumarate, malate, and succinate across the cytoplasmic membrane in a Na+-dependent manner. Furthermore, kinetic work predicts an ordered reaction sequence with Na+ (K 0.5 of 2.7 mM) binding before dicarboxylate (Km of 4.5 μM). Because this transporter and its mammalian homologs are functionally similar, we suggest that SdcS may serve as a useful model for DASS family structural analysis.

scholarly journals Characterization of a Catalase-Negative Methicillin-Resistant Staphylococcus aureus Strain

2007 ◽  
Vol 45 (8) ◽  
pp. 2684-2685 ◽  
Author(s):  
B. M. Gruner ◽  
S.-R. Han ◽  
H.-G. Meyer ◽  
U. Wulf ◽  
S. Bhakdi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Aureus Strain ◽  
Methicillin Resistant

Functional characterization of lipase in the pathogenesis of Staphylococcus aureus

2012 ◽  
Vol 419 (4) ◽  
pp. 617-620 ◽  
Author(s):  
Chunyan Hu ◽  
Ning Xiong ◽  
Yong Zhang ◽  
Simon Rayner ◽  
Shiyun Chen
Keyword(s):  
Staphylococcus Aureus ◽  
Functional Characterization

scholarly journals Structural and functional characterization of a novel type-III dockerin fromRuminococcus flavefaciens

FEBS Letters ◽  
2012 ◽  
Vol 587 (1) ◽  
pp. 30-36 ◽  
Author(s):  
Alon Karpol ◽  
Maroor K. Jobby ◽  
Michal Slutzki ◽  
Ilit Noach ◽  
Seth Chitayat ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Type Iii

scholarly journals Ectopic expression and functional characterization of type III polyketide synthase mutants from Emblica officinalis Gaertn

2016 ◽  
Vol 35 (10) ◽  
pp. 2077-2090 ◽  
Author(s):  
Girija Aiswarya ◽  
Vijayanathan Mallika ◽  
Luis A. J. Mur ◽  
Eppurathu Vasudevan Soniya
Keyword(s):  
Polyketide Synthase ◽  
Functional Characterization ◽  
Ectopic Expression ◽  
Emblica Officinalis ◽  
Type Iii Polyketide Synthase ◽  
Type Iii
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