Universal Real-Time PCR for the Detection and Quantification of Adeno-Associated Virus Serotype 2-Derived Inverted Terminal Repeat Sequences

2011 ◽  
pp. 110805114044008 ◽  
Author(s):  
Christine Aurnhammer ◽  
Maren Haase ◽  
Nadine Muether ◽  
Martin Hausl ◽  
Christina Rauschhuber ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Terminal Repeat ◽  
Inverted Terminal Repeat ◽  
Adeno Associated Virus ◽  
Repeat Sequences ◽  
Virus Serotype ◽  
Detection And Quantification

Universal Real-Time PCR for the Detection and Quantification of Adeno-Associated Virus Serotype 2-Derived Inverted Terminal Repeat Sequences

2012 ◽  
Vol 23 (1) ◽  
pp. 18-28 ◽  
Author(s):  
Christine Aurnhammer ◽  
Maren Haase ◽  
Nadine Muether ◽  
Martin Hausl ◽  
Christina Rauschhuber ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Terminal Repeat ◽  
Inverted Terminal Repeat ◽  
Adeno Associated Virus ◽  
Repeat Sequences ◽  
Virus Serotype ◽  
Detection And Quantification

Detection and Quantification of Bluetongue Virus Serotype 23 by Evagreen Based Real Time PCR

2018 ◽  
Vol 8 (5) ◽  
pp. 312
Author(s):  
Shabir Bhat ◽  
Jaynudin Khorajiya ◽  
Bilal Malla ◽  
Pervaiz Dar ◽  
Karam Singh ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bluetongue Virus ◽  
Virus Serotype ◽  
Detection And Quantification

scholarly journals Adeno-Associated Virus Serotype-Specific Inverted Terminal Repeat Sequence Role in Vector Transgene Expression

2020 ◽  
Vol 31 (3-4) ◽  
pp. 151-162 ◽  
Author(s):  
Lauriel F. Earley ◽  
Laura M. Conatser ◽  
Victoria M. Lue ◽  
Amanda L. Dobbins ◽  
Chengwen Li ◽  
...  
Keyword(s):  
Transgene Expression ◽  
Repeat Sequence ◽  
Terminal Repeat ◽  
Inverted Terminal Repeat ◽  
Adeno Associated Virus ◽  
Inverted Terminal Repeat Sequence ◽  
Virus Serotype ◽  
Terminal Repeat Sequence

scholarly journals Inverted terminal repeat sequences of adeno-associated virus enhance the antibody and CD8+ responses to a HIV-1 p55Gag/LAMP DNA vaccine chimera

Virology ◽  
2004 ◽  
Vol 323 (2) ◽  
pp. 220-232 ◽  
Author(s):  
Priya Chikhlikar ◽  
Luciana Barros de Arruda ◽  
Shikha Agrawal ◽  
Barry Byrne ◽  
William Guggino ◽  
...  
Keyword(s):  
Dna Vaccine ◽  
Terminal Repeat ◽  
Inverted Terminal Repeat ◽  
Adeno Associated Virus ◽  
Repeat Sequences ◽  
Hiv 1

scholarly journals Kinetics and Frequency of Adeno-Associated Virus Site-Specific Integration into Human Chromosome 19 Monitored by Quantitative Real-Time PCR

2002 ◽  
Vol 76 (15) ◽  
pp. 7554-7559 ◽  
Author(s):  
Daniela Hüser ◽  
Stefan Weger ◽  
Regine Heilbronn
Keyword(s):  
Human Chromosome ◽  
Real Time ◽  
Real Time Pcr ◽  
Transgenic Animals ◽  
Inverted Terminal Repeat ◽  
Quantitative Real Time Pcr ◽  
Chromosome 19 ◽  
Adeno Associated Virus ◽  
Vector Integration ◽  
Human Chromosome 19

ABSTRACT Adeno-associated virus type 2 (AAV-2) integrates specifically into a site on human chromosome 19 (chr-19) called AAVS1. To study the kinetics and frequency of chr-19-specific integration after AAV infection, we developed a rapid, sensitive, and quantitative real-time PCR assay for AAV inverted terminal repeat-chr-19-specific junctions. Despite the known variability of junction sites, conditions were established that ensured reliable quantification of integration rates within hours after AAV infection. The overall integration frequency was calculated to peak at between 10 and 20% of AAV-infected, unselected HeLa cells. At least 1 in 1,000 infectious AAV-2 particles was found to integrate site specifically up to day 4 postinfection in the absence of selection. Chromosomal breakpoints within AAVS1 agreed with those found in latently infected clonal cell lines and transgenic animals. Use of this quantitative real-time PCR will greatly facilitate the study of the early steps of wild-type and recombinant AAV vector integration.

scholarly journals Detection and quantification of Streptococcus pneumoniae from Iranian patients with pneumonia and individual carriers by real time PCR

2011 ◽  
Vol 10 (60) ◽  
pp. 12826-12832 ◽  
Author(s):  
Nomanpour Bizhan ◽  
Ghodousi Arash ◽  
Babaei Toraj ◽  
AliJavad Mousavi Seyd ◽  
Asadi Soroor ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Real Time ◽  
Real Time Pcr ◽  
Iranian Patients ◽  
Detection And Quantification

scholarly journals Detection of Peanut Allergen Traces with a Real Time PCR Assay - The Challenge to Protect Food-Allergic Consumers

2017 ◽  
Vol 7 (1) ◽  
pp. 32 ◽  
Author(s):  
Dimitra Houhoula ◽  
Stamatios Koussissis ◽  
Vladimiros Lougovois ◽  
John Tsaknis ◽  
Dimitra Kassavita ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Food Products ◽  
Molecular Techniques ◽  
Food Allergens ◽  
Pcr Assay ◽  
Peanut Allergen ◽  
Pcr Method ◽  
Detection And Quantification

The aim of the present study was the implementation of molecular techniques in the detection and quantification of allergic substances of peanut in various kinds of food products, e.g., breakfast cereals, chocolates and biscuits that are frequently related to allergies. In some cases, the presence of peanuts can be due to contamination during production and are not declared on the label. A total of 152 samples were collected from supermarkets and were analysed by a Real Time PCR method. The results indicated that 125 samples (83,3%) were found positive in peanut traces but the most important finding is that from the 84 samples that had no allergen declaration for peanuts, 48 (57,1%) of them were found positive. In conclusion, Real Time PCR can be a very important tool for the rapid detection and quantification of food allergens.

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