Production of Monoclonal Antibody Against Toxocara cati Second-Stage Larvae and Its Application for the Detection of Circulating Antigens

Hybridoma ◽  
2010 ◽  
Vol 29 (3) ◽  
pp. 217-220 ◽  
Author(s):  
Mohammad Zibaei ◽  
Seyed Mahmoud Sadjjadi ◽  
Satoko Ishiyama ◽  
Bahador Sarkari ◽  
Soji Uga
Keyword(s):  
Monoclonal Antibody ◽  
Toxocara Cati ◽  
Second Stage ◽  
Circulating Antigens

scholarly journals Immuno-PCR for Detection of Antigen to Angiostrongylus cantonensis Circulating Fifth-Stage Worms

2004 ◽  
Vol 50 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Soi-Moi Chye ◽  
Shiu-Ru Lin ◽  
Ya-Lei Chen ◽  
Lee-Yi Chung ◽  
Chuan-Min Yen
Keyword(s):  
Monoclonal Antibody ◽  
Cerebrospinal Fluid ◽  
Parasitic Nematode ◽  
Pcr Amplification ◽  
Definitive Diagnosis ◽  
Angiostrongylus Cantonensis ◽  
Promising Technique ◽  
Serum Samples ◽  
Wilcoxon Rank Sum Test ◽  
Circulating Antigens

Abstract Background: Definitive diagnosis of infestation with Angiostrongylus cantonensis is difficult because the parasitic nematode is undetectable in the cerebrospinal fluid (CSF) of one-half of afflicted patients and the diagnostic sensitivity of ELISA for circulating worm antigens in patient sera is low. We studied immuno-PCR as a diagnostic tool. Methods: We studied 30 controls and 60 afflicted patients (30 confirmed by parasitologic analysis of CSF). We used a monoclonal antibody to capture circulating A. cantonensis antigens in serum samples. A DNA label generated by PCR amplification with biotinylated primer was bound by use of streptavidin to a biotinylated third antibody. Circulating antigens sandwiched by monoclonal antibody were detected by PCR amplification of the DNA label. Results: The detection limit of the ELISA was 100–1000 times higher than that of the immuno-PCR. The concentrations of circulating antigens in patients were markedly higher than those in controls (Wilcoxon rank-sum test, P <0.001). At a cutoff of 0.1 ng/L, sensitivity and specificity for immunodiagnosis of patients with angiostrongyliasis by immuno-PCR were 98% (95% confidence interval, 91–99%) and 100% (93–100%), respectively. The test was positive in all parasitologically confirmed cases. Conclusions: Immuno-PCR is a promising technique for diagnosis of A. cantonensis infestation.

scholarly journals In vitro cultivation of Toxocara cati adult worms for production of eggs and evaluation of oviposition

2009 ◽  
Vol 46 (1) ◽  
pp. 28-30 ◽  
Author(s):  
M. Zibaei ◽  
S. Sadjjadi ◽  
B. Sarkari ◽  
A. Oryan ◽  
S. Uga
Keyword(s):  
Culture Method ◽  
Toxocara Canis ◽  
Clinical Syndrome ◽  
Egg Laying ◽  
In Vitro Cultivation ◽  
Toxocara Cati ◽  
Second Stage ◽  
Immunological Diagnosis ◽  
The Mean

AbstractToxocariasis is the clinical syndrome caused by infection of zoonotic roundworms of dogs (Toxocara canis) or cats (Toxocara cati). Current research on the immunology and pathology aspects of toxocariasis requires Toxocara second stage larvae and a ready source of excretory-secretory (ES) antigens. We cultured eleven pairs of both sexes of Toxocara cati adult worms maintained in RPMI 1640 medium in order to evaluate the amounts and duration of egg laying. At the first day and last day (day 19), the mean egg counts were 9300 and 250 eggs/ml, respectively. These results showed that this culture method is very appropriate for collection of pure oviposited eggs and/or production of adult ES antigens of Toxocara cati that could be used for immunological diagnosis of toxocariasis.

scholarly journals Microscophy Identification of Toxocara cati First Stage Larvae and Second Stage Larvae

2019 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Eny Coolfina Simarmata ◽  
Kusnoto Kusnoto ◽  
Mochamad Lazuardi ◽  
Setiawan Koesdarto ◽  
Endang Suprihati ◽  
...  
Keyword(s):  
Outer Layer ◽  
Light Microscope ◽  
Adult Stage ◽  
Anterior Part ◽  
Posterior Part ◽  
Middle Layer ◽  
Toxocara Cati ◽  
Structural Morphology ◽  
Second Stage ◽  
The Third

This study was aimed to identify the ultra structural morphology of Toxocara cati First Stage Larvae and Second Stage Larvae using Light Microscope. Toxocara cati larvae were obtained from adult worm eggs then were treated in phospat buffer saline with comparasion 1 : 1 until it reached the stage of larvae 1 and 2. The shell of egg Toxocara cati were thick and usually consist of three layers. The first layer was inner membrane, the second layer was middle layer and the third was outer layer. The results of larvae morphology that were identified using light microscope showed that the anterior part of Toxocara cati first stage larvae has a dorsal lip and esophagus and intestine on the posterior part but it could not be identified perfectly. The anterior part of Toxocara cati second stage larvae that were identified has three lips that leads directly into the oesophagus. Three lips on the anterior part of Toxocara cati larvae 2 consist of a dorsal lip and two subventral lips. Morphological of Toxocara cati larvae similar with the Toxocara cati adult worms because morphology of larvae and adult stage was difficult to differentiated.

scholarly journals Rapid Detection of a Schistosoma mansoniCirculating Antigen Excreted in Urine of Infected Individuals by Using a Monoclonal Antibody

1999 ◽  
Vol 37 (2) ◽  
pp. 354-357 ◽  
Author(s):  
Abdelfattah M. Attallah ◽  
Hisham Ismail ◽  
Samir A. El Masry ◽  
Hassan Rizk ◽  
Aia Handousa ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Infection Intensity ◽  
Rectal Biopsy ◽  
Urine Samples ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Parasitic Infections ◽  
Circulating Antigen ◽  
Circulating Antigens ◽  
Stool Analysis

Schistosoma circulating antigens were used to indicate the infection intensity and to assess cure. An immunoglobulin G2a (IgG2a) mouse monoclonal antibody was used in a fast dot-enzyme-linked immunosorbent assay (ELISA; FDA) for rapid and simple diagnosis of schistosomiasis in the field. Seven hundred Egyptians were parasitologically examined forSchistosoma mansoni and other parasitic infections. A rectal biopsy was done as a “gold standard” for individuals showing no S. mansoni eggs in their feces. Egg counts were obtained by the Kato smear method for only 100 of 152 individuals with eggs in their feces. Specific anti-schistosome IgG antibodies were evaluated in sera by ELISA. Urine samples from the 700 individuals were tested by FDA for detection of the circulating antigen. The assay showed a sensitivity of 93% among 433 infected individuals and a specificity of 89% among 267 noninfected individuals. FDA showed the highest efficiency of antigen detection (91%) compared with the efficiency of antibody detection by ELISA (75%) and stool analysis (60%). In addition, FDA detected infected patients with 20 eggs/g of feces. Also, the sensitivity of FDA ranged from 90 to 94% among samples from patients with different clinical stages of schistosomiasis. All the assay steps can be completed within 30 min at room temperature for 96 urine samples. The monoclonal antibody identified a 74-kDa antigen in different antigenic extracts of S. mansoni andSchistosoma haematobium and in the urine of infected individuals. In addition, a 30-kDa degradation product was identified only in the urine samples. On the basis of these results, FDA should be used as a rapid tool for the sensitive and specific diagnosis of Schistosoma infection.

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