Modification of gap junctional intercellular communication by changes in extracellular pH in Syrian hamster embryo cells

1990 ◽  
Vol 11 (6) ◽  
pp. 909-913 ◽  
Author(s):  
Randall J. Ruch ◽  
James E. Klaunig ◽  
James E. Klaunig ◽  
Gary A. Kerckaert ◽  
Robert A. LeBoeuf ◽  
...  
Keyword(s):  
Intercellular Communication ◽  
Syrian Hamster ◽  
Extracellular Ph ◽  
Gap Junctional Intercellular Communication ◽  
Hamster Embryo Cells ◽  
Embryo Cells ◽  
Gap Junctional

Heavy Metal Ions, Cytotoxicity and Gap Junctional Intercellular Communication in Syrian Hamster Embryo Cells

1992 ◽  
Vol 20 (2) ◽  
pp. 213-217
Author(s):  
Svein-Ole Mikalsen ◽  
Edgar Rivedal ◽  
Tore Sanner
Keyword(s):  
Heavy Metal ◽  
Intercellular Communication ◽  
Heavy Metal Ions ◽  
Cell Type ◽  
Gap Junctional Intercellular Communication ◽  
Cytotoxic Effects ◽  
Hamster Embryo Cells ◽  
Embryo Cells ◽  
Gap Junctional ◽  
Toxic Concentrations

The effects of NiSO4, K2CrO4, PbAc2, CrCl3 and CdAc2 on gap junctional intercellular communication (GJIC) were studied. Two exposure procedures were used — A: an established monolayer of cells was exposed to the compound, and B: the compound was mixed with the cells before seeding. The apparent inhibition of GJIC by CrCl3 and CdAc2 could be accounted for by cytotoxicity, i.e. disruption of physical contacts between cells. PbAc2 had no effect on GJIC. The effects of NiSO4 and K2CrO4 depended on the exposure procedure and cell type studied. Specifically, K2CrO4 induced a significant increase in GJIC in BPNi cells by procedure A, and a decrease by procedure B. The cytotoxic effects of NiSO4 were examined in more detail. The toxic concentrations varied widely between the methods used. The results indicate that the effect of compounds on GJIC may vary with different exposure procedures, and that inhibition of GJIC is not due to general cytotoxic effects.

scholarly journals Low pH enhances connexin32 degradation in the pancreatic acinar cell

2014 ◽  
Vol 307 (1) ◽  
pp. G24-G32 ◽  
Author(s):  
Anamika M. Reed ◽  
Thomas Kolodecik ◽  
Sohail Z. Husain ◽  
Fred S. Gorelick
Keyword(s):  
Gap Junctions ◽  
Acinar Cell ◽  
Intercellular Communication ◽  
Pancreatic Acinar Cell ◽  
Low Ph ◽  
Extracellular Ph ◽  
Gap Junctional Intercellular Communication ◽  
Junction Protein ◽  
Clinical Conditions ◽  
Gap Junctional

Decreased extracellular pH is observed in a number of clinical conditions and can sensitize to the development and worsen the severity of acute pancreatitis. Because intercellular communication through gap junctions is pH-sensitive and modulates pancreatitis responses, we evaluated the effects of low pH on gap junctions in the rat pancreatic acinar cell. Decreasing extracellular pH from 7.4 to 7.0 significantly inhibited gap junctional intracellular communication. Acidic pH also significantly reduced levels of connexin32, the predominant gap junction protein in acinar cells, and altered its localization. Increased degradation through the proteasomal, lysosomal, and autophagic pathways mediated the decrease in connexin32 under low-pH conditions. These findings provide the first evidence that low extracellular pH can regulate gap junctional intercellular communication by enhancing connexin degradation.

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