scholarly journals A New Total Antioxidant Potential Measurements Using RP-HPLC Assay with Fluorescence Detection

2011 ◽  
Vol 49 (5) ◽  
pp. 401-404 ◽  
Author(s):  
B. K. Glod ◽  
P. Piszcz ◽  
K. Czajka ◽  
P. K. Zarzycki
2012 ◽  
Vol 10 (6) ◽  
pp. 1786-1790 ◽  
Author(s):  
Paweł Wantusiak ◽  
Bronisław Głód

AbstractHPLC has been already used for the TAP estimation. Phenylalanine, salicylic, p-hydroxybenzoic (pHBA) or terephthalic (TPA) acids have been used as sensor compounds. Products of their reaction with the hydroxyl radicals, generated in the Fenton-like reaction, were analyzed using electrochemical or fluorescence detection. This paper describes the TAP assay based on the hydroxyl radicals reaction with pHBA, reversed-phase-HPLC separation and UV photometric detection. The elaborated assay has been used to evaluate TAP values of some apiculture products.


2012 ◽  
Vol 56 (2) ◽  
pp. 165-169 ◽  
Author(s):  
Katarzyna Ognik ◽  
Krzysztof Patkowski ◽  
Eugeniusz R. Grela

Abstract The experiment was conducted on lambs of the Polish Lowland sheep (PLS) and a synthetic BCP line. The redox potential was assessed in blood of lambs with different genotype and sex, which were fed feed mixtures with various contents of a proteinxanthophyll (PX) concentrate from alfalfa (1.5% and 3%). The 1.5% addition of PX concentrate to feed stimulated the response of the antioxidant defence mechanisms of the lambs to a greater extent than the 3% addition. The study showed that sex of the lambs had no significant effect on changes in the redox parameters of blood. The PLS genotype of lambs determined higher values of superoxide dismutase (SOD), total antioxidant potential of plasma (FRAP), malondialdehyde, and vitamin C than the BCP genotype. Along with the growth of the lambs, a descending tendency was observed in SOD and catalase levels, and an ascending tendency in FRAP value.


2003 ◽  
Vol 82 (3) ◽  
pp. 409-416 ◽  
Author(s):  
Rosana C Minussi ◽  
Massimo Rossi ◽  
Luciano Bologna ◽  
Lı́via Cordi ◽  
Domenico Rotilio ◽  
...  

2015 ◽  
Vol 173 ◽  
pp. 749-754 ◽  
Author(s):  
Bronisław K. Głód ◽  
Paweł M. Wantusiak ◽  
Paweł Piszcz ◽  
Elwira Lewczuk ◽  
Paweł K. Zarzycki

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