RP-HPLC, WITH FLUORESCENCE DETECTION, ASSAY FOR THE DETERMINATION OF TOTAL ANTIOXIDANT POTENTIAL (TAP)

2012 ◽  
Vol 35 (9) ◽  
pp. 1194-1201 ◽  
Author(s):  
Bronisław K. Głód ◽  
Paweł Piszcz ◽  
Agnieszka Beta ◽  
Paweł K. Zarzycki
Keyword(s):  
Fluorescence Detection ◽  
Antioxidant Potential ◽  
Rp Hplc ◽  
Detection Assay ◽  
Total Antioxidant ◽  
Total Antioxidant Potential

scholarly journals Application of UV detection in HPLC in the total antioxidant potential assay

2012 ◽  
Vol 10 (6) ◽  
pp. 1786-1790 ◽  
Author(s):  
Paweł Wantusiak ◽  
Bronisław Głód
Keyword(s):  
Hydroxyl Radicals ◽  
Fluorescence Detection ◽  
Reversed Phase ◽  
Uv Detection ◽  
Antioxidant Potential ◽  
Reversed Phase Hplc ◽  
Hplc Separation ◽  
Photometric Detection ◽  
Total Antioxidant ◽  
Total Antioxidant Potential

AbstractHPLC has been already used for the TAP estimation. Phenylalanine, salicylic, p-hydroxybenzoic (pHBA) or terephthalic (TPA) acids have been used as sensor compounds. Products of their reaction with the hydroxyl radicals, generated in the Fenton-like reaction, were analyzed using electrochemical or fluorescence detection. This paper describes the TAP assay based on the hydroxyl radicals reaction with pHBA, reversed-phase-HPLC separation and UV photometric detection. The elaborated assay has been used to evaluate TAP values of some apiculture products.

scholarly journals Effect of a Protein-Xanthophyll Concentrate from Alfalfa and of Genotype and Sex of Lambs on their Blood Redox Profile

2012 ◽  
Vol 56 (2) ◽  
pp. 165-169 ◽  
Author(s):  
Katarzyna Ognik ◽  
Krzysztof Patkowski ◽  
Eugeniusz R. Grela
Keyword(s):  
Superoxide Dismutase ◽  
Vitamin C ◽  
Redox Potential ◽  
Antioxidant Potential ◽  
Defence Mechanisms ◽  
Antioxidant Defence ◽  
Total Antioxidant ◽  
Total Antioxidant Potential

Abstract The experiment was conducted on lambs of the Polish Lowland sheep (PLS) and a synthetic BCP line. The redox potential was assessed in blood of lambs with different genotype and sex, which were fed feed mixtures with various contents of a proteinxanthophyll (PX) concentrate from alfalfa (1.5% and 3%). The 1.5% addition of PX concentrate to feed stimulated the response of the antioxidant defence mechanisms of the lambs to a greater extent than the 3% addition. The study showed that sex of the lambs had no significant effect on changes in the redox parameters of blood. The PLS genotype of lambs determined higher values of superoxide dismutase (SOD), total antioxidant potential of plasma (FRAP), malondialdehyde, and vitamin C than the BCP genotype. Along with the growth of the lambs, a descending tendency was observed in SOD and catalase levels, and an ascending tendency in FRAP value.

Phenolic compounds and total antioxidant potential of commercial wines

2003 ◽  
Vol 82 (3) ◽  
pp. 409-416 ◽  
Author(s):  
Rosana C Minussi ◽  
Massimo Rossi ◽  
Luciano Bologna ◽  
Lı́via Cordi ◽  
Domenico Rotilio ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Potential ◽  
Total Antioxidant ◽  
Total Antioxidant Potential

Method Validation for the Quantitative Analysis of Aflatoxins (B1, B2, G1, and G2) and Ochratoxin A in Processed Cereal-Based Foods by HPLC with Fluorescence Detection

2015 ◽  
Vol 98 (4) ◽  
pp. 939-945 ◽  
Author(s):  
Işil Gazioğlu ◽  
Ufuk Kolak
Keyword(s):  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Ochratoxin A ◽  
Fluorescence Detection ◽  
Aflatoxin B1 ◽  
Hplc Analysis ◽  
Extraction Procedure ◽  
Immunoaffinity Column ◽  
Rp Hplc

Abstract Modified AOAC 991.31 and AOAC 2000.03 methods for the simultaneous determination of total aflatoxins (AFs), aflatoxin B1, and ochratoxin A (OTA) in processed cereal-based foods by RP-HPLC coupled with fluorescence detection were validated. A KOBRA® Cell derivatization system was used to analyze total AFs. One of the modifications was the extraction procedure of mycotoxins. Both AFs and OTA were extracted with methanol–water (75 + 25, v/v) and purified with an immunoaffinity column before HPLC analysis. The modified methods were validated by measuring the specificity, selectivity, linearity, sensitivity, accuracy, repeatability, reproducibility, recovery, LOD, and LOQ parameters. The validated methods were successfully applied for the simultaneous determination of mycotoxins in 81 processed cereal-based foods purchased in Turkey. These rapid, sensitive, simple, and validated methods are suitable for the simultaneous determination of AFs and OTA in the processed cereal-based foods.

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