A Modified Analytical Method for Total Antioxidant Potential Assay Using RP-HPLC with Electrochemical Detection and Its Application for Pro- and Antioxidative Properties of Dopamine Measurement

AbstractThere is limited literature on the antioxidative properties of food of animal origin. Measurements of antioxidative properties are usually performed using the reaction of reduction of colored 2,2-diphenyl-1-picrylhydrazyl (DPPH) radials. Changes of the DPPH color are tracked photometrically. These measurements are interfered by both, the tested samples and reduced DPPH. This study aims to demonstrate the ability to separate different forms of DPPH (DPPH• and DPPH-H) by thin-layer chromatography (TLC). Further, it has been practically applied in the study of the determination of antioxidative properties of the meat samples. It was found that TLC can be used for the separation of different forms of DPPH as well as for measurement of TAP (total antioxidant potential) values related to the DPPH•. The strongest antioxidant properties were observed for pork neck extracted in buffer pH 2 and for smoked salmon fish extracted in acetone, the lowest for veal and turkey fillet extracted in methanol.

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RP-HPLC, WITH FLUORESCENCE DETECTION, ASSAY FOR THE DETERMINATION OF TOTAL ANTIOXIDANT POTENTIAL (TAP)

Journal of Liquid Chromatography &amp Related Technologies ◽

10.1080/10826076.2011.619029 ◽

2012 ◽

Vol 35 (9) ◽

pp. 1194-1201 ◽

Cited By ~ 5

Author(s):

Bronisław K. Głód ◽

Paweł Piszcz ◽

Agnieszka Beta ◽

Paweł K. Zarzycki

Keyword(s):

Fluorescence Detection ◽

Antioxidant Potential ◽

Rp Hplc ◽

Detection Assay ◽

Total Antioxidant ◽

Total Antioxidant Potential

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Effect of a Protein-Xanthophyll Concentrate from Alfalfa and of Genotype and Sex of Lambs on their Blood Redox Profile

Bulletin of the Veterinary Institute in Pulawy ◽

10.2478/v10213-012-0030-4 ◽

2012 ◽

Vol 56 (2) ◽

pp. 165-169 ◽

Cited By ~ 4

Author(s):

Katarzyna Ognik ◽

Krzysztof Patkowski ◽

Eugeniusz R. Grela

Keyword(s):

Superoxide Dismutase ◽

Vitamin C ◽

Redox Potential ◽

Antioxidant Potential ◽

Defence Mechanisms ◽

Antioxidant Defence ◽

Total Antioxidant ◽

Total Antioxidant Potential

Abstract The experiment was conducted on lambs of the Polish Lowland sheep (PLS) and a synthetic BCP line. The redox potential was assessed in blood of lambs with different genotype and sex, which were fed feed mixtures with various contents of a proteinxanthophyll (PX) concentrate from alfalfa (1.5% and 3%). The 1.5% addition of PX concentrate to feed stimulated the response of the antioxidant defence mechanisms of the lambs to a greater extent than the 3% addition. The study showed that sex of the lambs had no significant effect on changes in the redox parameters of blood. The PLS genotype of lambs determined higher values of superoxide dismutase (SOD), total antioxidant potential of plasma (FRAP), malondialdehyde, and vitamin C than the BCP genotype. Along with the growth of the lambs, a descending tendency was observed in SOD and catalase levels, and an ascending tendency in FRAP value.

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