scholarly journals Effect of a Protein-Xanthophyll Concentrate from Alfalfa and of Genotype and Sex of Lambs on their Blood Redox Profile

2012 ◽  
Vol 56 (2) ◽  
pp. 165-169 ◽  
Author(s):  
Katarzyna Ognik ◽  
Krzysztof Patkowski ◽  
Eugeniusz R. Grela

Abstract The experiment was conducted on lambs of the Polish Lowland sheep (PLS) and a synthetic BCP line. The redox potential was assessed in blood of lambs with different genotype and sex, which were fed feed mixtures with various contents of a proteinxanthophyll (PX) concentrate from alfalfa (1.5% and 3%). The 1.5% addition of PX concentrate to feed stimulated the response of the antioxidant defence mechanisms of the lambs to a greater extent than the 3% addition. The study showed that sex of the lambs had no significant effect on changes in the redox parameters of blood. The PLS genotype of lambs determined higher values of superoxide dismutase (SOD), total antioxidant potential of plasma (FRAP), malondialdehyde, and vitamin C than the BCP genotype. Along with the growth of the lambs, a descending tendency was observed in SOD and catalase levels, and an ascending tendency in FRAP value.

2019 ◽  
Vol 68 (1) ◽  
Author(s):  
Lailla Sabrina Queiroz Nazareno ◽  
Maria Raquel Alcântara de Miranda ◽  
Maria Ketully Neyane Alves Pinto ◽  
Mônica Maria Almeida Lopes ◽  
Maria Do Socorro Moura Rufino

The objective of this work was to identify and evaluate the bioactive compounds, as well as the antioxidant potential, present in mature fruits of cambuizeiro (Myrcia multiflora) from the Chapada do Araripe, Brazil.  In the fruits were evaluated: vitamin C, total anthocyanins, yellow flavonoids, total polyphenols, total antioxidant activity by the FRAP, DPPH and ABTS methods, and the antioxidant superoxide dismutase (SOD) and catalase (CAT) activity.  Cambui had an average vitamin C content of 56.40 mg/100 g, 2869.44 mg/100 g total anthocyanins 2858.50 mg/100 g yellow flavonoids and 1007.64 mg EAG/100 g of total polyphenols.  While the total antioxidant activity according to FRAP method was 123.94 μM ferrous sulphate/g, DPPH was 1805.66 g/g EC50, and ABTS was 32.62 μM trolox/g.  The enzymatic activity of SOD was 846,203 AU/g, while CAT did not read.  These results indicate that the mature fruits of cambuizeiro present high antioxidant potential, corroborating to the idea that their consumption may result in benefits to human health.


2015 ◽  
Vol 18 (4) ◽  
pp. 709-714 ◽  
Author(s):  
M. Merska ◽  
A. Czech ◽  
K. Ognik

Abstract The aim of the study was to assess the effect of different amounts of Yarrowia lipolytica yeast on the redox response and content of macro- and microelements in the blood plasma of turkey hens. The experiment was carried out on 240 turkey hens aged from 1 to 16 weeks. The birds were randomly assigned to 3 experimental groups of 80 birds each. Group I served as a control (K) and did not receive any experimental compounds. The turkey hens from the experimental groups (YL3 and YL6) were administered dried Yarrowia lipolytica yeast in their feed mixtures in the amount of 3% (YL3) or 6% (YL6). Yarrowia lipolytica yeast in the feed mixtures for the turkey hens did not induce oxidation reactions in the organism of the birds. However, an increase in catalase activity and a reduction in the level of LOOH, MDA and vitamin C were observed in the blood plasma of the turkey hens whose diet was supplemented with YL yeast. In the case of other indices, such as superoxide dismutase activity and total antioxidant potential (FRAP), the additive caused no significant changes. Administering Yarrowia lipolytica yeast to turkey hens may stimulate the enzymatic response of the antioxidant system (e.g. increasing catalase activity), mainly by increasing the concentration of iron in the plasma.


2015 ◽  
Vol 15 (1) ◽  
pp. 93-105 ◽  
Author(s):  
Katarzyna Ognik ◽  
Iwona Sembratowicz ◽  
Anna Czech ◽  
Ewelina Kulak ◽  
Malwina Merska

Abstract The aim of this study was to determine whether the introduction of stress affects corticosterone and malonyl dialdehyde levels and antioxidant indices of the blood of turkey hens, and whether additives used in conjunction with stress, such as aloe extract supplemented with trans-resveratrol and vitamin C or the amidrazone derivative 5-oxo-1,2,4-triazine, can mitigate adverse changes that may occur in these parameters. The experiment was carried out on 360 turkey hens allocated randomly to 6 groups of 60 birds each. Groups C and C(+)stress were the control groups and did not receive any additive. Birds from groups A and A(+)stress were administered aloe extract with the addition of trans-resveratrol and vitamin C in the amount of 0.70 ml/kg body weight (BW)/ day. The turkey hens from groups T and T(+)stress received 5-oxo-1,2,4-triazine in the amount of 30 μg/kg BW/day. Blood tests included determination of the content of corticosterone and malonyl dialdehyde (MDA), total plasma antioxidant potential (FRAP) and vitamin C, as well as activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT ) and glutathione peroxidase (PGx). The blood serum samples were also analysed for levels of zinc (Zn), copper (Cu) and iron (Fe) by atomic absorption spectrometry (AAS). The stress factors applied resulted in a significant increase in the level of corticosterone and MDA and in SOD and CAT activity in the blood plasma of the turkey hens. A significant decrease in the level of vitamin C and total plasma antioxidant potential were also recorded in the birds treated with stress. The aloe preparation supplemented with trans-resveratrol and vitamin C caused a significant increase in vitamin C, FRAP, Fe and Cu, and a decrease in the corticosterone and MDA levels in the blood plasma of the turkey hens, while in the blood plasma of birds treated with 5-oxo-1,2,4-triazine a significant increase was noted in superoxide dismutase activity and a decrease in the malonyl dialdehyde level. In order to alleviate the negative effect of stress, supplementation of diets with aloe extract supplemented with resveratrol and vitamin C may be considered.


2003 ◽  
Vol 82 (3) ◽  
pp. 409-416 ◽  
Author(s):  
Rosana C Minussi ◽  
Massimo Rossi ◽  
Luciano Bologna ◽  
Lı́via Cordi ◽  
Domenico Rotilio ◽  
...  

2015 ◽  
Vol 173 ◽  
pp. 749-754 ◽  
Author(s):  
Bronisław K. Głód ◽  
Paweł M. Wantusiak ◽  
Paweł Piszcz ◽  
Elwira Lewczuk ◽  
Paweł K. Zarzycki

2015 ◽  
Vol 167 ◽  
pp. 25-31 ◽  
Author(s):  
Ariana Bampouli ◽  
Konstantina Kyriakopoulou ◽  
Georgios Papaefstathiou ◽  
Vasiliki Louli ◽  
Nektarios Aligiannis ◽  
...  

Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 409 ◽  
Author(s):  
Mateusz Maciejczyk ◽  
Julita Szulimowska ◽  
Katarzyna Taranta-Janusz ◽  
Katarzyna Werbel ◽  
Anna Wasilewska ◽  
...  

Chronic kidney disease (CKD) is one of the most common modern-age diseases in children. Kidney failure does not reveal any symptoms for a long time; therefore, new biomarkers are sought, preferably those reflecting an early stage of CKD. The aim of our study was to evaluate total antioxidant potential as a biomarker differentiating the degree of CKD advancement. The study included 30 children with CKD and a control group matched by age and gender. Non-stimulated saliva (NWS), stimulated saliva (SWS), plasma and urine were used as study material. Total antioxidant potential was determined spectrophotometrically using the FRAP method (ferric ion reducing antioxidant parameter) by measuring total FRAP and uric acid (UA)-independent FRAP (FRAP-UA). We demonstrated that total FRAP, FRAP-UA and UA were significantly higher in stimulated saliva, as well as urine of CKD patients compared to the controls. These biomarkers increase with the progression of chronic kidney disease and their concentration in SWS reflects their content in urine. Interestingly, salivary FRAP and uric acid clearly differentiate between various stages of CKD as well as between healthy and ill children. Special attention should be paid to total FRAP which—measured in SWS—distinguishes patients with mildly to moderately decreased kidney function from those with severe renal impairment (AUC = 1, sensitivity = 100%, specificity = 100%). Although salivary FRAP may be a potential CKD biomarker in children, further studies are needed in a larger group of patients.


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