scholarly journals Application of UV detection in HPLC in the total antioxidant potential assay

2012 ◽  
Vol 10 (6) ◽  
pp. 1786-1790 ◽  
Author(s):  
Paweł Wantusiak ◽  
Bronisław Głód
Keyword(s):  
Hydroxyl Radicals ◽  
Fluorescence Detection ◽  
Reversed Phase ◽  
Uv Detection ◽  
Antioxidant Potential ◽  
Reversed Phase Hplc ◽  
Hplc Separation ◽  
Photometric Detection ◽  
Total Antioxidant ◽  
Total Antioxidant Potential

AbstractHPLC has been already used for the TAP estimation. Phenylalanine, salicylic, p-hydroxybenzoic (pHBA) or terephthalic (TPA) acids have been used as sensor compounds. Products of their reaction with the hydroxyl radicals, generated in the Fenton-like reaction, were analyzed using electrochemical or fluorescence detection. This paper describes the TAP assay based on the hydroxyl radicals reaction with pHBA, reversed-phase-HPLC separation and UV photometric detection. The elaborated assay has been used to evaluate TAP values of some apiculture products.

Reversed-Phase HPLC Separation of Human Serum Employing a Novel Saw-Tooth Gradient:  Toward Multidimensional Proteome Analysis

2004 ◽  
Vol 3 (6) ◽  
pp. 1149-1154 ◽  
Author(s):  
Daniel L. Morris, ◽  
Jennifer N. Sutton ◽  
Robert G. Harper ◽  
Aaron T. Timperman
Keyword(s):  
Human Serum ◽  
Proteome Analysis ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Hplc Separation

scholarly journals The Determination of Six Ionophore Coccidiostats in Feed by Liquid Chromatography with Postcolumn Derivatisation and Spectrofotometric/Fluorescence Detection

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Małgorzata Olejnik ◽  
Piotr Jedziniak ◽  
Teresa Szprengier-Juszkiewicz
Keyword(s):  
Drug Resistance ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Internal Standard ◽  
Reversed Phase ◽  
Feed Additives ◽  
Core Shell ◽  
Proficiency Tests ◽  
Reversed Phase Hplc

The control of levels of anticoccidial feed additives in targeted feeds plays an important role in the assurance of efficiency of animal treatment, prevention of drug resistance, and food safety. The robust and labour-efficient method for the simultaneous determination of six ionophore coccidiostats (lasalocid, maduramicin, monensin, narasin, salinomycin, and semduramicin) in targeted feed has been developed. Properly grinded and homogenized feed sample was spiked with internal standard (monesin methyl ester) and extracted with methanol. The extract was analysed with reversed phase HPLC without any further purification. The separation of the analytes with conventional C18 and core-shell columns was compared. Lasalocid was analysed with fluorescence detection, whereas other ionophores were detected with UV-Vis detector after derivatisation with vanillin in the presence of sulfuric acid. Fortified samples and targeted feeds at authorized levels were used for method validation. Recovery was in the range of 85–110%, depending on the analyte. The within-laboratory reproducibility did not exceed the target value from Horwitz equation. The results of the proficiency tests (z-scores in the range of −1.0 to 1.9) confirmed the reliability of the developed protocol.

scholarly journals Effect of a Protein-Xanthophyll Concentrate from Alfalfa and of Genotype and Sex of Lambs on their Blood Redox Profile

2012 ◽  
Vol 56 (2) ◽  
pp. 165-169 ◽  
Author(s):  
Katarzyna Ognik ◽  
Krzysztof Patkowski ◽  
Eugeniusz R. Grela
Keyword(s):  
Superoxide Dismutase ◽  
Vitamin C ◽  
Redox Potential ◽  
Antioxidant Potential ◽  
Defence Mechanisms ◽  
Antioxidant Defence ◽  
Total Antioxidant ◽  
Total Antioxidant Potential

Abstract The experiment was conducted on lambs of the Polish Lowland sheep (PLS) and a synthetic BCP line. The redox potential was assessed in blood of lambs with different genotype and sex, which were fed feed mixtures with various contents of a proteinxanthophyll (PX) concentrate from alfalfa (1.5% and 3%). The 1.5% addition of PX concentrate to feed stimulated the response of the antioxidant defence mechanisms of the lambs to a greater extent than the 3% addition. The study showed that sex of the lambs had no significant effect on changes in the redox parameters of blood. The PLS genotype of lambs determined higher values of superoxide dismutase (SOD), total antioxidant potential of plasma (FRAP), malondialdehyde, and vitamin C than the BCP genotype. Along with the growth of the lambs, a descending tendency was observed in SOD and catalase levels, and an ascending tendency in FRAP value.

Phenolic compounds and total antioxidant potential of commercial wines

2003 ◽  
Vol 82 (3) ◽  
pp. 409-416 ◽  
Author(s):  
Rosana C Minussi ◽  
Massimo Rossi ◽  
Luciano Bologna ◽  
Lı́via Cordi ◽  
Domenico Rotilio ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Potential ◽  
Total Antioxidant ◽  
Total Antioxidant Potential

scholarly journals Reversed-phase HPLC with UV detection for the determination of N-acetylaspartate and creatine

2005 ◽  
Vol 343 (1) ◽  
pp. 179-182 ◽  
Author(s):  
Mattias Tranberg ◽  
Malin H. Stridh ◽  
Barbro Jilderos ◽  
Stephen G. Weber ◽  
Mats Sandberg
Keyword(s):  
Reversed Phase ◽  
Uv Detection ◽  
Reversed Phase Hplc
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