scholarly journals Resolution of Enantiomers of (RS)-Baclofen by Ligand-Exchange Thin-Layer Chromatography

2016 ◽  
Vol 54 (5) ◽  
pp. 842-846 ◽  
Author(s):  
Manisha Singh ◽  
Poonam Malik ◽  
Ravi Bhushan
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ligand Exchange ◽  
Resolution Of Enantiomers ◽  
Layer Chromatography

Thin Layer Chromatography and in situ Fluorometry for the Quantitative Determination of Dimethoate and Malathion in Lettuce

1974 ◽  
Vol 57 (1) ◽  
pp. 165-167
Author(s):  
James D Macneil ◽  
Brian L Maclellan ◽  
Roland W Frep
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ligand Exchange ◽  
Palladium Complexes ◽  
Palladium Chloride ◽  
Fluorometric Determination ◽  
Cleanup Procedure ◽  
Layer Chromatography

Abstract Dimethoate and malathion in spiked lettuce extracts are separated by thin layer chromatography on silica gel followed by in situ fluorometric determination. Fluorescence is produced by spraying the developed chromatogram with a palladium chloride-calcein reagent. A ligand exchange occurs in which the palladium complexes preferentially with the sulfur in the pesticide, releasing the metal-fluorochromic indicator calcein. For dimethoate, a linear response is obtained for 10—100 ng/spot and the need for a preliminary cleanup procedure is eliminated.

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