Homogeneous enzyme immunoassay of serum digoxin with use of a bichromatic analyzer.

1979 ◽  
Vol 25 (1) ◽  
pp. 169-171 ◽  
Author(s):  
P B Eriksen ◽  
O Andersen
Keyword(s):  
Reaction Time ◽  
Enzyme Immunoassay ◽  
Emergency Situation ◽  
Palo Alto ◽  
Serum Digoxin ◽  
Immunoassay Technique ◽  
Homogeneous Enzyme Immunoassay ◽  
Assay Conditions

Abstract We applied the Enzyme Multiplied Immunoassay Technique (EMIT; Syva Corp., Palo Alto, CA) for determination of serum digoxin to the ABA-100 bichromatic analyzer. Assay conditions were almost exactly as prescribed for the manual procedure, but the ABA-100 offers high automation, smaller reagent volumes, and shorter reaction time. Precision studies gave CV's of less than 10%. Sixty patients' samples, analyzed for digoxin by radioimmunoassay and this enzyme immunoassay, gave a correlation (r) of 0.941. Results obtained with the ABA-100 were apparently slightly higher. One kit provides reagents for 250 assays, as compared to 70 assays with the manual procedure. In an emergency situation a result will be available about 60 min after the patient's sample is received; one operator can analyze about 120 samples in 8 h.

scholarly journals Comparison of Radioimmunoassay with Homogeneous Enzyme Immunoassay for Determination of Serum Digoxin

1981 ◽  
Vol 7 (5) ◽  
pp. 274-278
Author(s):  
MAKOTO IKEDA ◽  
KATSUO TAKAHASHI ◽  
KUNIHIKO FUJIWARA ◽  
EIJI TAKAHASHI ◽  
SINTARO OYABU ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Serum Digoxin ◽  
Homogeneous Enzyme Immunoassay

Enzyme channeling immunoassay: A new homogeneous enzyme immunoassay technique

1980 ◽  
Vol 106 (1) ◽  
pp. 223-229 ◽  
Author(s):  
David J. Litman ◽  
Teresa M. Hanlon ◽  
Edwin F. Ullman
Keyword(s):  
Enzyme Immunoassay ◽  
Immunoassay Technique ◽  
Homogeneous Enzyme Immunoassay ◽  
Enzyme Immunoassay Technique

scholarly journals Application of Insolubilized Antibody-Enzyme Immunoassay Technique to Determination of Antiepileptic Drugs in Body Fluids

1980 ◽  
Vol 6 (1) ◽  
pp. 29-35 ◽  
Author(s):  
KAZUYO NISHIHARA ◽  
YUKINAO KOHDA ◽  
YUKIYA SAITOH ◽  
FUJIO NAKAGAWA ◽  
ZENZO TAMURA
Keyword(s):  
Enzyme Immunoassay ◽  
Antiepileptic Drugs ◽  
Body Fluids ◽  
Immunoassay Technique ◽  
Enzyme Immunoassay Technique

scholarly journals Study on determination of serum digoxin by radial partition fluorometric enzyme immunoassay.

1988 ◽  
Vol 14 (6) ◽  
pp. 443-447
Author(s):  
MARI MIYAZAKI ◽  
KAZUHIKO ARIMORI ◽  
MASAHIRO NAKANO
Keyword(s):  
Enzyme Immunoassay ◽  
Serum Digoxin

Semiautomated homogeneous enzyme immunoassay of total serum thyroxine with a kinetic analyzer.

1980 ◽  
Vol 26 (3) ◽  
pp. 501-503
Author(s):  
H Bhatia
Keyword(s):  
Enzyme Immunoassay ◽  
Total Serum ◽  
Serum Thyroxine ◽  
Assay Procedure ◽  
Homogeneous Enzyme Immunoassay

Abstract A new semiautomatic procedure for determination of total serum thyroxine was adapted to a kinetic analyzer, LKB 2086 Mark II. The ABA thyroxine assay kit was used, and although the assay procedure was modified, the advantage of small reagent volumes, short measuring times, and automation were retained. The results were analyzed off-line with a programmable desk-top calculator. The method has a precision of 7% (CV) and a sensitivity of 8 nmol/L. Values in sera from 64 patients analyzed by enzyme immunoassay and by radioimmunoassay correlated well (r = 0.975). One kit contains enough reagents for 500 assays, and an operator could do 25 samples (i.e., 64 assays) in about 4 h.

Comparison of Radioimmunoassay with Homogeneous Enzyme Immunoassay for the Determination of Theophylline Concentration in Serum

1979 ◽  
Vol 1 (3) ◽  
pp. 381-386 ◽  
Author(s):  
Roger L. Forrester ◽  
Jacqueline Worrall ◽  
William R. Robertson ◽  
Lester J. Wataji ◽  
David Wilhelm
Keyword(s):  
Enzyme Immunoassay ◽  
Theophylline Concentration ◽  
Homogeneous Enzyme Immunoassay

Stability of standard curves prepared for EMIT homogeneous enzyme immunoassay kits stored at room temperature after reconstitution.

1980 ◽  
Vol 26 (5) ◽  
pp. 652-654 ◽  
Author(s):  
P R Bach ◽  
J W Larsen
Keyword(s):  
Enzyme Immunoassay ◽  
Room Temperature ◽  
Palo Alto ◽  
Parallel Increase ◽  
Absorbance Changes ◽  
The Stability ◽  
Time Standard ◽  
Homogeneous Enzyme Immunoassay ◽  
Product Literature

Abstract We examined the stability of standard curves obtained with use of homogeneous enzyme immunoassay reagents (EMIT; Syva Corp., Palo Alto, CA) for assay of lidocaine, procainamide, N-acetylprocainamide, gentamicin, and theophylline, when stored at room temperature (23-24 degrees C) after reconstitution of the lyophilized materials. Standards were run and curves obtained for as long as 16 days after reconstitution. All standard curves were acceptable, according to the criteria specified in Syva product literature. Absorbance changes for all calibrators, including the zero calibrator, increased gradually with time. Increases of non-zero calibrators were largely offset by a parallel increase in the zero calibrator, such that the quantity delta A--delta A0 was very nearly constant with time. Standard curves based on the quantity delta A--delta A0 can be used for longer than curves based only on delta A.

Long-wavelength homogeneous enzyme immunoassay for the determination of amikacin in water samples

Talanta ◽  
2009 ◽  
Vol 78 (1) ◽  
pp. 305-309 ◽  
Author(s):  
M SANCHEZMARTINEZ ◽  
M AGUILARCABALLOS ◽  
A GOMEZHENS
Keyword(s):  
Enzyme Immunoassay ◽  
Water Samples ◽  
Long Wavelength ◽  
Homogeneous Enzyme Immunoassay
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