Photodegradation of Lipofuscin in Suspension and in ARPE-19 Cells and the Similarity of Fluorescence of the Photodegradation Product with Oxidized Docosahexaenoate

Retinal lipofuscin accumulates with age in the retinal pigment epithelium (RPE), where its fluorescence properties are used to assess retinal health. It was observed that there is a decrease in lipofuscin fluorescence above the age of 75 years and in the early stages of age-related macular degeneration (AMD). The purpose of this study was to investigate the response of lipofuscin isolated from human RPE and lipofuscin-laden cells to visible light, and to determine whether an abundant component of lipofuscin, docosahexaenoate (DHA), can contribute to lipofuscin fluorescence upon oxidation. Exposure of lipofuscin to visible light leads to a decrease in its long-wavelength fluorescence at about 610 nm, with a concomitant increase in the short-wavelength fluorescence. The emission spectrum of photodegraded lipofuscin exhibits similarity with that of oxidized DHA. Exposure of lipofuscin-laden cells to light leads to a loss of lipofuscin granules from cells, while retaining cell viability. The spectral changes in fluorescence in lipofuscin-laden cells resemble those seen during photodegradation of isolated lipofuscin. Our results demonstrate that fluorescence emission spectra, together with quantitation of the intensity of long-wavelength fluorescence, can serve as a marker useful for lipofuscin quantification and for monitoring its oxidation, and hence useful for screening the retina for increased oxidative damage and early AMD-related changes.

Variations in Earth's 1D viscosity structure in different tectonic regimes

Earth’s long-wavelength geoid provides insights into the thermal, structural, and compositional evolution of the mantle. Historically, most estimates of mantle viscosity using the long-wavelength geoid have considered radial variations with depth in a symmetric Earth. Global estimates of this kind suggest an increase in viscosity from the upper mantle to lower mantle of roughly 2 -- 3 orders of magnitude. Using a spatio-spectral localization technique with the geoid, here we estimate a series of locally constrained viscosity-depth profiles covering two unique regions, the Pacific and Atlantic hemispheres, which show distinct rheological properties. The Pacific region exhibits the conventional Earth's 1D rheology with a factor of roughly 80-100 increase in viscosity occurring at transition zone depths (400 - 800 km). The Atlantic region in contrast does not show significant viscosity jumps with depth, and instead has a near uniform viscosity in the top 1000~km. The inferred viscosity variations between our two regions could be due to the prevalence of present-day subduction in the Pacific and the infrequence of slabs in the Atlantic, combined with a possible hydrated transition zone and mid-mantle of the Atlantic region by ancient subduction during recent supercontinent cycles. Rigid slab material within the top 800 km, with about 90\% Majoritic garnet in the form of subducted oceanic crust, coupled with unique regional mantle structures, may be generating a strong transition zone viscosity interface for the Pacific region. These effective lateral variations in mantle viscosity could play a role in the observed deformation differences between the Pacific and Atlantic hemispheres.

The Abundances of Zinc and Copper in the Atmospheres of 78 Vir (A2p SrCrEu) and θ Leo (A2 IV)

Zinc and Copper abundances are rarely available for normal and Chemically Peculiar A stars because the strongest transitions of Zn ii and Cu ii fall in the mid-UV. Estimates of the abundances of zinc and copper are derived for 78 Vir (A2p SrCrEu) and θ Leo (A2 IV) using mean mid-ultraviolet spectra constructed by coadding individual spectra collected with the Long Wave Prime and Long wavelength Redundant cameras over the 18 yr of the IUE mission. The strong transition of Cu ii at 2135.98 Å is present in 78 Vir and θ Leo but definitely stronger in 78 Vir, whereas all Zn ii lines are blended. Spectral synthesis of the least blended lines yields estimates of the abundances of zinc and copper of about 4.92 and 4.95 respectively in θ Leo and 5.82 and 5.19 in 78 Vir (on a scale where log(H) = 12). There is no convincing evidence that these lines varied in the spectra analyzed for both stars.

Gyro-average method for global gyrokinetic particle simulation in realistic tokamak geometry

Gyro-average is a crucial operation to capture the essential finite Larmor radius effect (FLR) in gyrokinetic simulation. In order to simulate strongly shaped plasmas, an innovative multi-point average method based on non-orthogonal coordinates has been developed to improve the accuracy of the original multi-point average method in gyrokinetic particle simulation. This new gyro-average method has been implemented in the gyrokinetic toroidal code (GTC). Benchmarks have been carried out to prove the accuracy of this new method. In the limit of concircular tokamak, ion temperature gradient (ITG) instability is accurately recovered for this new method and consistency is achieved. The new gyro-average method is also used to solve the gyrokinetic Poisson equation, and its correctness has been confirmed in the long wavelength limit for realistic shaped plasmas. The improved GTC code with the new gyro-average method has been used to investigate the ITG instability with EAST magnetic geometry. The simulation results show that the correction induced by this new method in the linear growth rate is more significant for short wavelength modes where the finite Larmor radius (FLR) effect becomes important. Due to its simplicity and accuracy, this new gyro-average method can find broader applications in simulating the shaped plasmas in realistic tokamaks.

First-principles identification of VI+Cui defect cluster in cuprous iodide: origin of red light photoluminescence

The γ-phase Cuprous Iodide (CuI) emerges as a promising transparent p-type semiconductor for next-generation display technology because of its wide direct band gap, intrinsic p-type conductivity, and high carrier mobility. Two main peaks are observed in its photoluminescence (PL). One is short wavelength (410-430 nm) emission, which is well attributed to the electronic transitions at Cu vacancy, whereas the other long wavelength emission (680-720 nm) has not been fully understood. In this paper, through first-principles simulations, we investigate the formation energies and emission line shape for various defects, and discover that the intrinsic point defect cluster V_I+Cu_i^(2+) is the source of the long wavelength emission. Our finding is further supported by the prediction that the defect concentration decreases dramatically as the chemical condition changes from Cu-rich to I-rich, explaining the significant reduction in the red light emission if CuI is annealed in abundant I environment.

Cornering the universal shape of fluctuations

Understanding the fluctuations of observables is one of the main goals in science, be it theoretical or experimental, quantum or classical. We investigate such fluctuations in a subregion of the full system, focusing on geometries with sharp corners. We report that the angle dependence is super-universal: up to a numerical prefactor, this function does not depend on anything, provided the system under study is uniform, isotropic, and correlations do not decay too slowly. The prefactor contains important physical information: we show in particular that it gives access to the long-wavelength limit of the structure factor. We exemplify our findings with fractional quantum Hall states, topological insulators, scale invariant quantum critical theories, and metals. We suggest experimental tests, and anticipate that our findings can be generalized to other spatial dimensions or geometries. In addition, we highlight the similarities of the fluctuation shape dependence with findings relating to quantum entanglement measures.

Guest-host doped strategy for constructing ultralong-lifetime near-infrared organic phosphorescence materials for bioimaging

Organic near-infrared room temperature phosphorescence materials have unparalleled advantages in bioimaging due to their excellent penetrability. However, limited by the energy gap law, the near-infrared phosphorescence materials (>650 nm) are very rare, moreover, the phosphorescence lifetimes of these materials are very short. In this work, we have obtained organic room temperature phosphorescence materials with long wavelengths (600/657–681/732 nm) and long lifetimes (102–324 ms) for the first time through the guest-host doped strategy. The guest molecule has sufficient conjugation to reduce the lowest triplet energy level and the host assists the guest in exciton transfer and inhibits the non-radiative transition of guest excitons. These materials exhibit good tissue penetration in bioimaging. Thanks to the characteristic of long lifetime and long wavelength emissive phosphorescence materials, the tumor imaging in living mice with a signal to background ratio value as high as 43 is successfully realized. This work provides a practical solution for the construction of organic phosphorescence materials with both long wavelengths and long lifetimes.

The Abundances of Cobalt and Nickel in the Atmosphere of 78 Vir (A2p SrCrEu)

Cobalt and nickel abundances are rarely available for normal and Chemically Peculiar A stars because the strongest transitions of Co ii and Ni ii fall in the mid-UV. The abundances of cobalt and nickel are derived for 78 Vir using a mean mid-ultraviolet spectrum constructed by coadding 10 spectra collected with the Long Wave Prime and Long wavelength Redundant cameras over the 18 yr of the IUE mission. The strong transitions of Co ii at 2286.16 Å, 2307.86 Å, 2324.32 Å and 2580.33 Å and that of Ni ii et 2287.09 Å are present and more or less affected by blends. The least blended, λ 2286.16 Å, yields a mean overabundance of cobalt of 5 times the solar abundance, the Ni ii line at 2287.09 Å yields a 3 times solar overabundance. There is no convincing evidence that these lines varied in the spectra analyzed. The rotational period of 78 Vir estimated from its recent TESS lightcurve is 3.723 ± 0.055 days.

Secondary Through-Space Interactions: Achieving Single-Molecule White-Light Emission from Clusteroluminogens with Isolated Phenyl Rings

Clusteroluminogens (CLgens) refer to some non-conjugated molecules that show visible light due to the formation of aggregates and unique electronic properties with through-space interactions (TSI). Although mature and systematic theories of molecular photophysics have been developed to study conventional conjugated chromophores, it is still challenging to endow CLgens with designed photophysical properties by manipulating TSI. Herein, three CLgens with non-conjugated donor-acceptor structures and different halide substituents with secondary TSI are designed and synthesized. These molecules show multiple emissions and even white-light emission in the crystalline state and the intensity ratio of these multiple emission peaks is easily manipulated by changing the halide atom and excitation wavelength. Experimental and theoretical results successfully disclose the electronic nature of these multiple emissions: through-space conjugation for short-wavelength fluorescence, through-space charge transfer based on secondary TSI for long-wavelength fluorescence, and room-temperature phosphorescence. The introduction of secondary TSI to CLgens not only enriches their varieties of photophysical properties but also inspires the establishment of novel aggregate photophysics for clusteroluminescence.