Isoelectric focusing of serum proteins on modified thin cellulose-acetate membranes.

1979 ◽  
Vol 25 (7) ◽  
pp. 1320-1322 ◽  
Author(s):  
J Ambler ◽  
G Walker
Keyword(s):  
Cellulose Acetate ◽  
Isoelectric Focusing ◽  
Boron Trifluoride ◽  
Serum Proteins ◽  
Ph Gradients ◽  
Specific Identification ◽  
Cellulose Acetate Membranes ◽  
Protein Staining

Abstract We applied a method previously described for treating cellulsoe acetate gel strips with boron trifluoride in methanol, to make them suitable for isoelectric focusing of proteins, to dry cellulose acetate membranes. The results obtained are shown to compare very favorably. Both wide and narrow pH gradients may be used and coupled either to conventional protein staining or to specific identification by immunofixation. Focusing ordinarily takes 90--120 min, is technically easy, and is economical in the use of ampholytes.

Genetic Variants of Alpha 1-Antitrypsin and Hemoglobin Typed by Isoelectric Focusing in Preselected Narrow pH Gradients with Phastsystem

1992 ◽  
Vol 38 (4) ◽  
pp. 577-580 ◽  
Author(s):  
J O Jeppsson ◽  
R Einarsson
Keyword(s):  
Isoelectric Focusing ◽  
Genetic Variants ◽  
Clinical Medicine ◽  
Ph Gradients ◽  
Total Analysis Time ◽  
Fast Screening ◽  
Abnormal Hemoglobin ◽  
Total Analysis ◽  
Protein Staining ◽  
Alpha 1 Antitrypsin

Abstract In this method for automatically running and staining isoelectric focusing (IEF) gels, pre-made dehydrated polyacrylamide gels were rehydrated before assays run with the PhastSystem (Pharmacia LKB Biotechnology). The typing of genetic variants of hemoglobin and alpha 1-antitrypsin in narrow pH gradients (pH 6.7-7.7 and 4.2-4.9, respectively) was simple, convenient, and reproducible. The clinically important variants of alpha 1-antitrypsin (ZZ and SZ) were identified from serum or dried blood on filter paper. The fast screening of abnormal hemoglobin samples (HbS) for cases in clinical medicine was easily performed. The total analysis time for the phenotyping with conventional protein staining was approximately 60 min.

An improved method of high-voltage isoelectric focusing on cellulose acetate membranes

1988 ◽  
Vol 9 (3) ◽  
pp. 149-150 ◽  
Author(s):  
Tosifusa Toda ◽  
Kiyoko Sano-Shiba ◽  
Hiroko Cho ◽  
Poh Lin Soon ◽  
Makoto Nakao ◽  
...  
Keyword(s):  
Cellulose Acetate ◽  
Isoelectric Focusing ◽  
High Voltage ◽  
Improved Method ◽  
Cellulose Acetate Membranes

Evaluation of a Cellulose-Acetate Electrophoresis System for Serum Protein Fractionation

1965 ◽  
Vol 11 (10) ◽  
pp. 937-942 ◽  
Author(s):  
Alex Kaplan ◽  
John Savory
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Blood Donors ◽  
Serum Proteins ◽  
Normal Values ◽  
Protein Fractionation ◽  
Cellulose Acetate Electrophoresis ◽  
Cellulose Acetate Membranes ◽  
Control Serum ◽  
Electrophoresis System

Abstract A rapid system for the quantitative fractionation of serum proteins by electrophoresis on cellulose-acetate membranes was evaluated and found to be quite precise. The reproducibility (coefficient of variation) of the routine fractionation of a control serum carried out 40 times during a 15-week period was 2.4% for albumin and 14.2, 6.0, 6.1, and 5.2%, respectively, for the α1-, α2-, β-, and γ-globulin fractions. Normal values are given for serum protein fractions (specimens from nonprofessional blood donors) obtained by cellulose acetate electrophoresis.

Isoelectric focusing of milk-clotting enzymes

1977 ◽  
Vol 44 (1) ◽  
pp. 69-72 ◽  
Author(s):  
P. G. Righetti ◽  
Bruna M. Molinari ◽  
G. Molinari
Keyword(s):  
Thin Layer ◽  
Cellulose Acetate ◽  
Isoelectric Focusing ◽  
Polyacrylamide Gel ◽  
Acidic Ph ◽  
Ph Gradients ◽  
Milk Clotting ◽  
Cellulose Acetate Film ◽  
Ph Range ◽  
Highly Porous

SummaryIsoelectric focusing in thin-layer polyacrylamide gel has been applied successfully to the characterization and identification of calf rennet and its substitutes. The use of acidic pH gradients (pH range 2·5–6) allows the identification of calf and microbial rennets and bovine and pig pepsins.A new, very rapid and sensitive zymogram technique has been developed by overlaying the gel with a highly porous, transparent cellulose acetate film impregnated with casein.

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