Isoelectric focusing of milk-clotting enzymes

1977 ◽  
Vol 44 (1) ◽  
pp. 69-72 ◽  
Author(s):  
P. G. Righetti ◽  
Bruna M. Molinari ◽  
G. Molinari
Keyword(s):  
Thin Layer ◽  
Cellulose Acetate ◽  
Isoelectric Focusing ◽  
Polyacrylamide Gel ◽  
Acidic Ph ◽  
Ph Gradients ◽  
Milk Clotting ◽  
Cellulose Acetate Film ◽  
Ph Range ◽  
Highly Porous

SummaryIsoelectric focusing in thin-layer polyacrylamide gel has been applied successfully to the characterization and identification of calf rennet and its substitutes. The use of acidic pH gradients (pH range 2·5–6) allows the identification of calf and microbial rennets and bovine and pig pepsins.A new, very rapid and sensitive zymogram technique has been developed by overlaying the gel with a highly porous, transparent cellulose acetate film impregnated with casein.

INSTABILITY OF pH GRADIENTS FORMED BY ISOELECTRIC FOCUSING IN POLYACRYLAMIDE GEL

1973 ◽  
Vol 209 (1 Isoelectric F) ◽  
pp. 44-64 ◽  
Author(s):  
A. Chrambach ◽  
P. Doerr ◽  
G. R. Finlayson ◽  
L. E. M. Miles ◽  
D. Rodbard ◽  
...  
Keyword(s):  
Isoelectric Focusing ◽  
Polyacrylamide Gel ◽  
Ph Gradients

Isoelectric focusing of serum proteins on modified thin cellulose-acetate membranes.

1979 ◽  
Vol 25 (7) ◽  
pp. 1320-1322 ◽  
Author(s):  
J Ambler ◽  
G Walker
Keyword(s):  
Cellulose Acetate ◽  
Isoelectric Focusing ◽  
Boron Trifluoride ◽  
Serum Proteins ◽  
Ph Gradients ◽  
Specific Identification ◽  
Cellulose Acetate Membranes ◽  
Protein Staining

Abstract We applied a method previously described for treating cellulsoe acetate gel strips with boron trifluoride in methanol, to make them suitable for isoelectric focusing of proteins, to dry cellulose acetate membranes. The results obtained are shown to compare very favorably. Both wide and narrow pH gradients may be used and coupled either to conventional protein staining or to specific identification by immunofixation. Focusing ordinarily takes 90--120 min, is technically easy, and is economical in the use of ampholytes.

scholarly journals Separation of intermediates in the refolding of reduced erabutoxin b by analytical isoelectric focusing in layers of polyacrylamide gel

1982 ◽  
Vol 201 (3) ◽  
pp. 495-499 ◽  
Author(s):  
Françoise Bouet ◽  
André Ménez ◽  
Robert C. Hider ◽  
Pierre Fromageot
Keyword(s):  
Thin Layer ◽  
Isoelectric Focusing ◽  
Polyacrylamide Gel ◽  
Suitable Method ◽  
Erabutoxin B ◽  
Snake Neurotoxin ◽  
Low Rate

Isoelectric focusing in a thin layer of polyacrylamide gel is shown to be a suitable method for the resolution of intermediates trapped during the refolding process of reduced cystine-containing proteins. The method has been applied to the well-characterized snake neurotoxin erabutoxin b. An explanation is offered for the relatively low rate of refolding of this polypeptide.

An anodic drift of pH gradients in isoelectric focusing on polyacrylamide gel

1978 ◽  
Vol 88 (1) ◽  
pp. 186-195 ◽  
Author(s):  
Nga Y. Nguyen ◽  
Alice G. McCormick ◽  
Andreas Chrambach
Keyword(s):  
Isoelectric Focusing ◽  
Polyacrylamide Gel ◽  
Ph Gradients

Urinary Proteins Studied by Use of Isoelectric Focusing. I. Tubular Malfunction in Association with Exposure to Cadmium

1973 ◽  
Vol 19 (10) ◽  
pp. 1179-1183 ◽  
Author(s):  
O Vesterberg ◽  
G Nise
Keyword(s):  
Thin Layer ◽  
Isoelectric Focusing ◽  
Polyacrylamide Gel ◽  
Cadmium Exposure ◽  
Chronic Pyelonephritis ◽  
Agarose Gel ◽  
Urinary Proteins ◽  
Protein Patterns ◽  
Β2 Microglobulin ◽  
Additional Information

Abstract Isoelectric focusing in thin-layer polyacrylamide gel has been applied to studies of urinary proteins. We could differentiate the following patterns: tubular, glomerular with high and low selectivity, as well as mixed glomerular and tubular patterns, as seen in some cases of chronic pyelonephritis and uremia. The typical tubular-protein patterns—i.e., as developed in association with cadmium exposure—were characterized by elevated excretion of β2-microglobulin. Possibly the severity of renal malfunction can be assessed by the isoelectric focusing technique. To identify the protein zones, we in some cases complemented isoelectric focusing with electrophoresis at right angles in acrylamide gel and also in agarose gel containing antibodies to provide additional information.

Generic Identification of Cooked and Frozen Crabmeat by Thin Layer Polyacrylamide Gel Isoelectric Focusing: Collaborative Study

1981 ◽  
Vol 64 (3) ◽  
pp. 670-673
Author(s):  
Judith Krzynowek ◽  
Kate Wiggin ◽  
◽  
M G Allsup ◽  
C Annand ◽  
...  
Keyword(s):  
Thin Layer ◽  
Isoelectric Focusing ◽  
Collaborative Study ◽  
Polyacrylamide Gel ◽  
Gel Isoelectric Focusing

Abstract Ten collaborators were required to focus the ureaextracted proteins from 14 crabmeat samples. Six of the samples were knowns and were labeled as to their species. Eight samples were unidentified as to species and were labeled with letters. The 8 unknowns were identified by comparing the focused patterns with the patterns of the 6 species-labeled crabmeat samples. Seventy-nine samples were identified with 97.5% accuracy. This method has been adopted official first action.

Sign in / Sign up

Export Citation Format

Share Document