Four electrophoretic methods compared for diagnosis of type III hyperlipoproteinemia.

1979 ◽  
Vol 25 (8) ◽  
pp. 1471-1475 ◽  
Author(s):  
K Kuba ◽  
K Lippel ◽  
I D Frantz
Keyword(s):  
Family Study ◽  
Paper Electrophoresis ◽  
Polyacrylamide Gel ◽  
The Other ◽  
Lipid Research ◽  
Central Laboratory ◽  
Type Iii ◽  
First Degree Relatives ◽  
Type Iii Hyperlipoproteinemia ◽  
Precipitation Technique

Abstract Blood drawn from 192 probands and 1129 first-degree relatives who were participants in a collaborative family study of hyperlipoproteinemia at nine Lipid Research Clinics was used to prepare aliquots of whole plasma and top (d less than 1.006 g/mL) and bottom (d greater than 1.006 g/mL) ultracentrifugal fractions. Each aliquot was analyzed at a central laboratory by electrophoresis on paper, agarose, and polyacrylamide gel, and by a combined electrophoretic precipitation technique. The electrophoretograms were evaluated for the presence or absence of a "floating-beta" lipoprotein band. All four methods agreed completely for 92.3% of the samples. An additional 2.0% of the samples were in agreement for three electrophoretic methods, but the paper electrophoretic results were not interpretable. Another 1.9% were considered to be "floating-beta" positive by paper electrophoresis but negative by the other three electrophoretic methods.

scholarly journals Macrophage Infiltration into the Glomeruli in Lipoprotein Glomerulopathy

2015 ◽  
Vol 5 (3) ◽  
pp. 204-212 ◽  
Author(s):  
Satoshi Takasaki ◽  
Kunihiko Maeda ◽  
Kensuke Joh ◽  
Shu Yamakage ◽  
Sachiko Fukase ◽  
...  
Keyword(s):  
The Other ◽  
Macrophage Infiltration ◽  
Lipoprotein Glomerulopathy ◽  
Clinical Behavior ◽  
Foamy Macrophage ◽  
Histopathological Features ◽  
Type Iii ◽  
Type Iii Hyperlipoproteinemia ◽  
Apo E ◽  
Foamy Macrophages

Lipoprotein glomerulopathy (LPG) is characterized by histopathological features showing intra-glomerular lipoprotein thrombi and type III hyperlipoproteinemia (HLP), with heterozygote mutation of apolipoprotein (apo) E gene. On the other hand, as another renal lipidosis with type III HLP, apoE2 homozygote-related glomerulopathy (apoE2-GN) showing foamy macrophages has been reported. The case of a 25-year-old man who had LPG by clinical behavior and gene analysis, but demonstrated atypical histopathological features with a substantial amount of foamy macrophage infiltration in the glomeruli, is presented. The combination of alleles for apoE Tokyo/Maebashi and classical apoE2 (Arg158Cys) was inferred to be the leading cause of the unique renal pathology with lipoprotein thrombi and foamy macrophages. In addition, foamy macrophages infiltrated some part of the apoE-positive region within the glomerulus, but did not exist in lipoprotein thrombi despite apoE positivity, suggesting that properties of apoE are crucial in the development of LPG rather than macrophage function. This case provides important information related to the pathogenesis of LPG and apoE2-GN.

Inheritance of type-III hyperlipoproteinemia. Lipoprotein patterns in first-degree relatives

Metabolism ◽  
1977 ◽  
Vol 26 (3) ◽  
pp. 225-254 ◽  
Author(s):  
Bengt Vessby ◽  
H. Hedstrand ◽  
L-G. Lundin ◽  
U. Olsson
Keyword(s):  
Type Iii ◽  
First Degree Relatives ◽  
Type Iii Hyperlipoproteinemia

A Family Study of Two Subgroups of Schizoaffective Patients

1989 ◽  
Vol 154 (5) ◽  
pp. 640-643 ◽  
Author(s):  
Mario Maj
Keyword(s):  
High Risk ◽  
Psychiatric Disorders ◽  
Schizoaffective Disorder ◽  
Clinical Picture ◽  
Affective Disorders ◽  
Family Study ◽  
The Other ◽  
Schizoaffective Disorders ◽  
First Degree Relatives ◽  
Group A

A family study was carried out in two groups of patients fulfilling RDC for schizoaffective disorder: in one, a full affective and a full schizophrenic syndrome were simultaneously present; in the other, affective and schizophrenic features appeared within a polymorphic and rapidly changing clinical picture, with depersonalisation/derealisation and/or confusion. In the first-degree relatives of patients of the former group, the risk of major psychiatric disorders was not significantly different from that of relatives of schizophrenics, whereas in the first-degree relatives of patients of the latter group a low risk for both schizophrenia and major affective disorders, and a relatively high risk for schizoaffective disorders, were observed.

Isoelectric focusing of insulin, 125I-insulin and the 125I-insulin-antibody complex

1979 ◽  
Vol 44 (6) ◽  
pp. 1828-1834
Author(s):  
Asja Šiševa ◽  
Jiřina Slaninová ◽  
Tomislav Barth ◽  
Stephan P. Ditzov ◽  
Luben M. Sirakov
Keyword(s):  
Isoelectric Focusing ◽  
Polyacrylamide Gel ◽  
The Other ◽  
Insulin Antibody ◽  
Isoelectric Points ◽  
Antibody Complex ◽  
And Storage ◽  
Acid Region ◽  
Three Components

Isoelectric focusing on polyacrylamide gel columns of three native crystalline commercial preparations of insulin and 125I-labelled insulin was carried out. All the compounds studied contained three components of different isoelectric points. The largest fraction, having pI 5.60 ± 0.05, was common to all preparations. The other two fractions were situated in the acid region of pH between pI 4.5 and 5.2. The presence of these fractions is explained by the contamination of crystalline insulins by proinsulin and by the formation of des-amido derivatives during the dissolving and storage of insulin samples, and, in case of labelled insulin, also by the presence of heavily iodinated insulin and contaminating components. The isoelectric focusing of the complex 125I-insulin-antibody showed a peak of radioactivity having pI 6.15 ± 0.05.

Type I Collagen Biosynthesis by Skin Fibroblasts from Patients with Idiopathic Juvenile Osteoporosis

1995 ◽  
Vol 89 (1) ◽  
pp. 69-73 ◽  
Author(s):  
Andrew E. Pocock ◽  
Martin J. O. Francis ◽  
Roger Smith
Keyword(s):  
Osteogenesis Imperfecta ◽  
Type I Collagen ◽  
The Other ◽  
Type I ◽  
Osteogenesis Imperfecta Type ◽  
Unrelated Control ◽  
Type Iii ◽  
Collagen Peptides ◽  
Idiopathic Juvenile Osteoporosis ◽  
Osteogenesis Imperfecta Type I

1. Skin fibroblast lines were cultured from nine patients who had the features of idiopathic juvenile osteoporosis, six relatives, five unrelated control subjects and three unrelated patients with osteogenesis imperfecta type I. Some patients with idiopathic juvenile osteoporosis were adults whose previous osteoporosis was in remission. Two patients with idiopathic juvenile osteoporosis were siblings and one patient with idiopathic juvenile osteoporosis had a daughter with severe osteogenesis imperfecta (type III). 2. The ratio of type III to type I collagen, synthesized by fibroblasts, was increased in two of the patients with osteogenesis imperfecta type I and in the daughter with osteogenesis imperfecta type III, but was normal in all the other patients with idiopathic juvenile osteoporosis and the other relatives. 3. Radiolabelled collagen was digested by cyanogen bromide and separated on SDS-PAGE. Unreduced collagen peptides migrated normally, except those from both the two siblings with idiopathic juvenile osteoporosis. In these two lines, abnormal migration suggested the presence of collagen I mutations. 4. The secretion of synthesized collagen by these two idiopathic juvenile osteoporosis lines and two others was reduced to only 43–45% as compared with a line from a 13-year-old control subject, which was defined as 100%. The three osteogenesis imperfecta type I lines secreted 18–37%, the other five idiopathic juvenile osteoporosis lines secreted 57–75%, the relatives (including the daughter with severe osteogenesis imperfecta) secreted 49–115% and the controls secreted 69–102%. 5. We conclude that qualitative abnormalities of type I collagen associated with a reduction in total secreted collagen synthesis may occur in a minority of patients with idiopathic juvenile osteoporosis; these patients could represent a subset of patients with this disorder.

scholarly journals Studies of familial type III hyperlipoproteinemia using as a genetic marker the apoE phenotype E2/2

1982 ◽  
Vol 23 (8) ◽  
pp. 1224-1235
Author(s):  
J L Breslow ◽  
V I Zannis ◽  
T R SanGiacomo ◽  
J L Third ◽  
T Tracy ◽  
...  
Keyword(s):  
Genetic Marker ◽  
Type Iii ◽  
Type Iii Hyperlipoproteinemia ◽  
Familial Type ◽  
Apoe Phenotype

Maudsley family study: Whole brain volume changes in sporadic schizophrenics and their first degree relatives

2000 ◽  
Vol 41 (1) ◽  
pp. 126
Author(s):  
A. Grech ◽  
T. Toulopoulou ◽  
T. Sharma ◽  
J. Yakeley ◽  
R.M. Murray
Keyword(s):  
Family Study ◽  
Brain Volume ◽  
Volume Changes ◽  
Whole Brain ◽  
First Degree Relatives
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