scholarly journals Macrophage Infiltration into the Glomeruli in Lipoprotein Glomerulopathy

2015 ◽  
Vol 5 (3) ◽  
pp. 204-212 ◽  
Author(s):  
Satoshi Takasaki ◽  
Kunihiko Maeda ◽  
Kensuke Joh ◽  
Shu Yamakage ◽  
Sachiko Fukase ◽  
...  
Keyword(s):  
The Other ◽  
Macrophage Infiltration ◽  
Lipoprotein Glomerulopathy ◽  
Clinical Behavior ◽  
Foamy Macrophage ◽  
Histopathological Features ◽  
Type Iii ◽  
Type Iii Hyperlipoproteinemia ◽  
Apo E ◽  
Foamy Macrophages

Lipoprotein glomerulopathy (LPG) is characterized by histopathological features showing intra-glomerular lipoprotein thrombi and type III hyperlipoproteinemia (HLP), with heterozygote mutation of apolipoprotein (apo) E gene. On the other hand, as another renal lipidosis with type III HLP, apoE2 homozygote-related glomerulopathy (apoE2-GN) showing foamy macrophages has been reported. The case of a 25-year-old man who had LPG by clinical behavior and gene analysis, but demonstrated atypical histopathological features with a substantial amount of foamy macrophage infiltration in the glomeruli, is presented. The combination of alleles for apoE Tokyo/Maebashi and classical apoE2 (Arg158Cys) was inferred to be the leading cause of the unique renal pathology with lipoprotein thrombi and foamy macrophages. In addition, foamy macrophages infiltrated some part of the apoE-positive region within the glomerulus, but did not exist in lipoprotein thrombi despite apoE positivity, suggesting that properties of apoE are crucial in the development of LPG rather than macrophage function. This case provides important information related to the pathogenesis of LPG and apoE2-GN.

Apo E variants in patients with type III hyperlipoproteinemia

1996 ◽  
Vol 127 (2) ◽  
pp. 273-282 ◽  
Author(s):  
Fernando Civeira ◽  
Miguel Pocoví ◽  
Ana Cenarro ◽  
Elena Casao ◽  
Elisabet Vilella ◽  
...  
Keyword(s):  
Type Iii ◽  
Type Iii Hyperlipoproteinemia ◽  
Apo E

Four electrophoretic methods compared for diagnosis of type III hyperlipoproteinemia.

1979 ◽  
Vol 25 (8) ◽  
pp. 1471-1475 ◽  
Author(s):  
K Kuba ◽  
K Lippel ◽  
I D Frantz
Keyword(s):  
Family Study ◽  
Paper Electrophoresis ◽  
Polyacrylamide Gel ◽  
The Other ◽  
Lipid Research ◽  
Central Laboratory ◽  
Type Iii ◽  
First Degree Relatives ◽  
Type Iii Hyperlipoproteinemia ◽  
Precipitation Technique

Abstract Blood drawn from 192 probands and 1129 first-degree relatives who were participants in a collaborative family study of hyperlipoproteinemia at nine Lipid Research Clinics was used to prepare aliquots of whole plasma and top (d less than 1.006 g/mL) and bottom (d greater than 1.006 g/mL) ultracentrifugal fractions. Each aliquot was analyzed at a central laboratory by electrophoresis on paper, agarose, and polyacrylamide gel, and by a combined electrophoretic precipitation technique. The electrophoretograms were evaluated for the presence or absence of a "floating-beta" lipoprotein band. All four methods agreed completely for 92.3% of the samples. An additional 2.0% of the samples were in agreement for three electrophoretic methods, but the paper electrophoretic results were not interpretable. Another 1.9% were considered to be "floating-beta" positive by paper electrophoresis but negative by the other three electrophoretic methods.

Lipoprotein glomerulopathy-like disease in a patient with type III hyperlipoproteinemia due to apolipoprotein E2 (Arg158 Cys)/3 heterozygosity

2007 ◽  
Vol 11 (2) ◽  
pp. 174-179 ◽  
Author(s):  
Miho Karube ◽  
Kimimasa Nakabayashi ◽  
Yasunori Fujioka ◽  
Ken Yoshihara ◽  
Akira Yamada ◽  
...  
Keyword(s):  
Lipoprotein Glomerulopathy ◽  
Type Iii ◽  
Type Iii Hyperlipoproteinemia

scholarly journals Diminished LDL Receptor and High Heparin Binding of Apolipoprotein E2 Sendai Associated with Lipoprotein Glomerulopathy

2001 ◽  
Vol 12 (3) ◽  
pp. 524-530
Author(s):  
MICHAEL M. HOFFMANN ◽  
HUBERT SCHARNAGL ◽  
ELEFTHERIA PANAGIOTOU ◽  
WERNER T. BANGHARD ◽  
HEINRICH WIELAND ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Low Density Lipoprotein ◽  
Ldl Receptor ◽  
Density Lipoprotein ◽  
Heparin Binding ◽  
Lipoprotein Glomerulopathy ◽  
Dominant Type ◽  
Type Iii ◽  
Type Iii Hyperlipoproteinemia ◽  
Heparin Binding Domain

Abstract. Variants of apolipoprotein E (apoE) have been linked to lipoprotein glomerulopathy, a new glomerular disease characterized by the deposition of lipoproteins in mesangial capillaries. One third of affected patients are heterozygous for apoE2 Sendai (Arg145 Pro). Variants of apoE can also produce type III hyperlipoproteinemia (HLP). Recessive type III HLP is caused by apoE2 (Arg158 Cys), a mutant with diminished low-density lipoprotein (LDL) receptor binding but halfnormal heparin binding. Dominant type III HLP is caused by mutations that markedly alter heparin binding but modestly reduce receptor binding. This study examined whether apoE2 Sendai (Arg145 Pro) was functionally different from type III HLP-producing apoE variants by expressing apoE3, apoE2 (Arg158 Cys), apoE1 (Arg146 Glu), a dominant apoE variant, and apoE2 Sendai (Arg145 Pro) in the baculovirus system. LDL receptor binding was studied using recombinant apoE complexed to phospholipid vesicles and to very lowdensity lipoprotein from a patient with familiar apoE deficiency. Compared with apoE3, receptor-binding activities of apoE2 (Arg158 Cys), apoE1 (Arg146 Glu), and apoE2 Sendai (Arg145 Pro) all were less than 5%. Heparin-binding activities were 53%, 23%, and 66%, respectively, of apoE3. The distribution of apoE2 Sendai among the major plasma lipoprotein fractions was similar to that of apoE3 and apoE2 (Arg158 Cys). ApoE2 Sendai (Arg145 Pro) represents the only known mutation within the heparin-binding domain of apoE (residues 142 through 147), revealing diminished receptor binding and almost normal heparin binding. These unique characteristics of apoE2 Sendai (Arg145 Pro) may relate to the development of lipoprotein glomerulopathy.

scholarly journals A competitive reverse transcription–PCR to study apolipoprotein ε gene expression

1998 ◽  
Vol 44 (4) ◽  
pp. 773-778 ◽  
Author(s):  
Martin Rexin ◽  
Giso Feussner
Keyword(s):  
Gene Expression ◽  
Reverse Transcription ◽  
Physiological Mechanism ◽  
Human Monocyte ◽  
Type Iii ◽  
Reverse Transcription Pcr ◽  
Type Iii Hyperlipoproteinemia ◽  
Apo E ◽  
Polymerase Chain ◽  
Hyperlipoproteinemia Type Iii

Abstract We developed a rapid and simple competitive reverse transcription-polymerase chain reaction for the quantification of apoε mRNA in human monocyte-derived macrophages. The method was applied, and its reliability was shown in patients with the familial lipoprotein disorder, type III hyperlipoproteinemia. Type III hyperlipoproteinemic patients express markedly higher concentrations of apoε mRNA when compared with healthy controls. Patients with this disease are usually (>90%) homozygous for a receptor binding-defective isoform of apolipoprotein apo E (apo E2). The higher expression of apoε mRNA in the patients could, therefore, be a physiological mechanism to compensate for functionally defective apo E. The developed procedure might be valuable in assessment of apoε gene expression in human disease.

scholarly journals A Case of a 9-year-old Girl with Homozygote, Apo E 2/2 in whom Type III Hyperlipoproteinemia Occurred after an Operation of the Craniopharyngioma

1989 ◽  
Vol 17 (1) ◽  
pp. 133-139 ◽  
Author(s):  
Hiroshi NAKATA ◽  
Masaaki ETO ◽  
Akizuki MORIKAWA ◽  
Kiyoshi WATANABE ◽  
Kaneo ISHII ◽  
...  
Keyword(s):  
Type Iii ◽  
Type Iii Hyperlipoproteinemia ◽  
Apo E
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