Applicability of various quality-control sera to assay of high-density lipoprotein cholesterol.

Abstract Effective internal quality control and external quality assessment of high-density lipoprotein cholesterol assay is made difficult by analyte instability, and the suitability of quality-control sera for this purpose has not been studied. We have therefore investigated the properties of 25 different control sera from 15 suppliers by estimating within-batch precision for the two precipitation procedures used most widely (phosphotungstate/Mg2+ and heparin/Mn2+ with enzymic measurement of cholesterol. Some sera had properties similar to those of fresh human serum, but others demonstrated poor precision for one or both procedures or contained apparent high-density lipoprotein cholesterol in unphysiological concentrations. A study of six sera indicated that between-batch precision was consistent with the within-batch findings. We found that eight of the 25 batches of quality-control serum we investigated may be used for internal quality control and external quality assessment of high-density lipoprotein cholesterol assay.

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Applicability of various quality-control sera to assay of high-density lipoprotein cholesterol.

Clinical Chemistry ◽

10.1093/clinchem/26.7.903 ◽

1980 ◽

Vol 26 (7) ◽

pp. 903-907 ◽

Cited By ~ 3

Author(s):

D G Bullock ◽

T J Carter ◽

S V Hughes

Keyword(s):

Quality Control ◽

High Density Lipoprotein ◽

Quality Assessment ◽

High Density Lipoprotein Cholesterol ◽

External Quality Assessment ◽

Density Lipoprotein ◽

High Density ◽

Lipoprotein Cholesterol ◽

Internal Quality ◽

External Quality

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Stability of frozen liquid control sera for assay of cholesterol in high-density lipoprotein.

Clinical Chemistry ◽

10.1093/clinchem/28.1.155 ◽

1982 ◽

Vol 28 (1) ◽

pp. 155-157 ◽

Cited By ~ 11

Author(s):

P N Demacker ◽

A G Hijmans ◽

D F van Sommeren-Zondag ◽

A P Jansen

Keyword(s):

Quality Control ◽

High Density Lipoprotein ◽

High Density Lipoprotein Cholesterol ◽

Density Lipoprotein ◽

Cholesterol Content ◽

Storage Conditions ◽

High Density ◽

Lipoprotein Cholesterol ◽

Internal Quality ◽

Lipid Control

Abstract Liquid serum pools with low-, normal-, or above-normal concentration of high-density lipoprotein cholesterol were prepared by selection and dilution of sera having low lipoprotein content or by enrichment with concentrated high-density lipoprotein. Stability of the serum pools depended on storage conditions. On storage between -2 and -12 degrees C, the high-density lipoprotein cholesterol content decreased. At a constant temperature of -19 to -21 degrees C the concentrations remained stable for more than 18 months. The imprecision (CV) of the high-density lipoprotein cholesterol assay during this period as established with enzymic cholesterol analysis (Clin. Chem. 26: 1780-1786, 1980) of these serum pools was between 2.7 and 4.8% (n = 51). Serum pools prepared and stored as described are suitable for internal quality-control procedures. In external quality-control trials these sera may be superior to the commercially available lyophilized lipid control sera.

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Quality control in assay of high-density lipoprotein cholesterol.

Clinical Chemistry ◽

10.1093/clinchem/26.1.0177 ◽

1980 ◽

Vol 26 (1) ◽

pp. 177-178

Author(s):

W Hohenwallner ◽

R Sommer ◽

E Wimmer ◽

G V Tschurtschenthaler

Keyword(s):

Quality Control ◽

High Density Lipoprotein ◽

High Density Lipoprotein Cholesterol ◽

Density Lipoprotein ◽

High Density ◽

Lipoprotein Cholesterol

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Quality control in assay of high-density lipoprotein cholesterol.

Clinical Chemistry ◽

10.1093/clinchem/26.1.177 ◽

1980 ◽

Vol 26 (1) ◽

pp. 177-178 ◽

Cited By ~ 1

Author(s):

W Hohenwallner ◽

R Sommer ◽

E Wimmer ◽

G V Tschurtschenthaler

Keyword(s):

Quality Control ◽

High Density Lipoprotein ◽

High Density Lipoprotein Cholesterol ◽

Density Lipoprotein ◽

High Density ◽

Lipoprotein Cholesterol

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Triglyceride to high density lipoprotein cholesterol ratio, total cholesterol to high density lipoprotein cholesterol ratio and low ankle brachial index in an elderly population

VASA ◽

10.1024/0301-1526/a000348 ◽

2014 ◽

Vol 43 (3) ◽

pp. 189-197 ◽

Cited By ~ 10

Author(s):

Yiqiang Zhan ◽

Jinming Yu ◽

Rongjing Ding ◽

Yihong Sun ◽

Dayi Hu

Keyword(s):

High Density Lipoprotein ◽

Total Cholesterol ◽

High Density Lipoprotein Cholesterol ◽

Ankle Brachial Index ◽

Density Lipoprotein ◽

High Density ◽

Lipoprotein Cholesterol ◽

Lipid Lowering ◽

Cholesterol Ratio ◽

Potential Biomarker

Background: The associations of triglyceride (TG) to high-density lipoprotein cholesterol ratio (HDL‑C) and total cholesterol (TC) to HDL‑C ratio and low ankle brachial index (ABI) were seldom investigated. Patients and methods: A population based cross-sectional survey was conducted and 2982 participants 60 years and over were recruited. TG, TC, HDL‑C, and low-density lipoprotein cholesterol (LDL-C) were assessed in all participants. Low ABI was defined as ABI ≤ 0.9 in either leg. Multiple logistic regression models were applied to study the association between TG/HDL‑C ratio, TC/HDL‑C ratio and low ABI. Results: The TG/HDL‑C ratios for those with ABI > 0.9 and ABI ≤ 0.9 were 1.28 ± 1.20 and 1.48 ± 1.13 (P < 0.0001), while the TC/HDL‑C ratios were 3.96 ± 1.09 and 4.32 ± 1.15 (P < 0.0001), respectively. After adjusting for age, gender, body mass index, obesity, current drinking, physical activity, hypertension, diabetes, lipid-lowering drugs, and cardiovascular disease history, the odds ratios (ORs) with 95 % confidence intervals (CIs) of low ABI for TG/HDL‑C ratio and TC/HDL‑C ratio were 1.10 (0.96, 1.26) and 1.34 (1.14, 1.59) in non-smokers. When TC was further adjusted, the ORs (95 % CIs) were 1.40 (0.79, 2.52) and 1.53 (1.21, 1.93) for TG/HDL‑C ratio and TC/HDL‑C ratio, respectively. Non-linear relationships were detected between TG/HDL‑C ratio and TC/HDL‑C ratio and low ABI in both smokers and non-smokers. Conclusions: TC/HDL‑C ratio was significantly associated with low ABI in non-smokers and the association was independent of TC, TG, HDL‑C, and LDL-C. TC/HDL‑C might be considered as a potential biomarker for early peripheral arterial disease screening.

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