Liquid-chromatographic measurement of creatinine in serum and urine.

1983 ◽  
Vol 29 (5) ◽  
pp. 851-853 ◽  
Author(s):  
T Okuda ◽  
T Oie ◽  
M Nishida
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
External Standard ◽  
Chromatographic Measurement ◽  
Liquid Chromatographic ◽  
Serum And Urine

Abstract We describe the adaptation of a "high-performance" liquid chromatographic method for determination of creatinine in serum and urine. The proposed method is simple, rapid, precise, and accurate. The retention time for creatinine can be varied simply by changing the KH2PO4 concentration in the mobile phase: acetonitrile/aqueous KH2PO4 (1/4 by vol). Within-day precision (CV) was 1.2-3.6% in serum chromatographed with an internal standard, and 2.3-2.8% in serum when an external standard was used. Between-day precision (CV) was 1.3-2.1% in serum and 1.3-2.7% in urine (with an external standard). Analytical recoveries of creatinine added to serum were 94-100% for the method with an internal standard, 95-103% with an external standard.

Liquid-chromatographic measurement of nitrazepam in plasma.

1982 ◽  
Vol 28 (7) ◽  
pp. 1478-1481 ◽  
Author(s):  
H Kelly ◽  
A Huggett ◽  
S Dawling
Keyword(s):  
Complete Resolution ◽  
High Performance ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Small Sample ◽  
Ultraviolet Detector ◽  
Liquid Chromatographic Method ◽  
Chromatographic Measurement ◽  
The Stability ◽  
Liquid Chromatographic

Abstract In this simple and rapid "high-performance" liquid-chromatographic method for determining nitrazepam in plasma, serum, or whole blood, the sample at pH 7.4 is extracted into diethyl ether with an internal standard (prazepam), chromatographed, and detected at 280 nm with a fixed-wavelength ultraviolet detector. A specimen, together with standards and a quality control, can be analyzed in duplicate within 90 min. The limit of sensitivity is 5 micrograms/L (nitrazepam and 7-acetamidonitrazepam) and 50 micrograms/L (7-aminonitrazepam), and no interferents have been found. This method has the advantages of a small sample requirement and complete resolution of nitrazepam and the above-mentioned major metabolites. We have used this method for analysis of therapeutic and overdose concentrations of nitrazepam, and to investigate the stability of the drug in blood.

Liquid-chromatographic determination of amitryptyline and its metabolites in serum, with adsorption onto glass minimized.

1982 ◽  
Vol 28 (10) ◽  
pp. 2143-2148 ◽  
Author(s):  
P M Edelbroek ◽  
E J de Haas ◽  
F A de Wolff
Keyword(s):  
High Performance ◽  
Internal Standard ◽  
Isoamyl Alcohol ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Irreversible Adsorption ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract To study correlations between the concentrations, in serum, of amitriptyline and its most important metabolites with clinical response in patients, we developed a "high-performance" liquid-chromatographic method for routine determination of amitriptyline, nortriptyline, total 10-hydroxy-amitriptyline, desmethylnortriptyline, and E(trans)- and Z(cis)-10-hydroxynortriptyline. These compounds are extracted from 1 mL of alkalinized serum into hexane/isoamyl alcohol (99/1 by vol). Perazine is the internal standard. To minimize irreversible adsorption of the drugs onto the glassware, 5 micrograms of maprotiline is added to the organic phase just before evaporation. After a 10-min resolution on a silica column eluted with acetonitrile/methanol/NH4OH (1 mol/L), absorbance is measured at 240 nm. Only chlorimipramine, doxepin, procainamide, and N-acetylprocainamide may interfere with assay of the compounds that probably are therapeutically relevant: amitriptyline, nortriptyline, and E-10-hydroxynortriptyline. Uremia, lipemia, and icterus also do not affect the analysis.

scholarly journals DEVELOPMENT AND VALIDATION OF A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD DETERMINATION OF ZIDOVUDINE ENCAPSULATED IN PCL NANOPARTICLES

2017 ◽  
Vol 1 (2) ◽  
pp. 1-8
Author(s):  
Milena Cristina Ribeiro Souza Magalhães ◽  
Alisson Samuel Portes Caldeira ◽  
Hanna De Sousa Rocha Almeida ◽  
Sílvia Ligório Fialho ◽  
Armando Da Silva Cunha Junior
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Hplc Method ◽  
Liquid Chromatographic Method ◽  
Development And Validation ◽  
Liquid Chromatographic ◽  
Isocratic Mode

A reversed-phase high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of encapsulation efficiency of zidovudine in nanoparticules. The method was carried out in isocratic mode using 0.040M sodium acetate: methanol: acetonitrile: glacial acetic acid (880:100:20:2) as mobile phase, a C8 column at 25ºC and UV detection at 240 nm. The method was linear (r2 ˃ 0.99) over the range of 25.0-150.0 μg/mL, precise (RSD ˂ 5%), accurate (recovery = 100.5%), robust and selective. The validated HPLC-UV method can be successfully applied to determine the rate of zidovudine in nanoparticules.

A high-performance liquid-chromatographic method for routine simultaneous determination of nicotine and cotinine in plasma.

1988 ◽  
Vol 34 (4) ◽  
pp. 724-729 ◽  
Author(s):  
M Hariharan ◽  
T VanNoord ◽  
J F Greden
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
High Performance ◽  
Methylene Chloride ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Standard Curve ◽  
Liquid Chromatographic

Abstract We describe a rapid, sensitive method for the routine simultaneous determination of nicotine and cotinine in 1 mL of plasma. Extraction in 10-mL screw-capped Teflon tubes with methylene chloride after deproteinization with trichloroacetic acid eliminated emulsion formation. The extract, after evaporation and reconstitution in 30 microL of mobile phase, is injected into a reversed-phase C-18 ion-pair column of an isocratic high-performance liquid-chromatographic unit. Absorbance is monitored at 256 nm. The mobile phase is a citrate-phosphate (30 mmol each per liter) buffer mixture containing 50 mL of acetonitrile and 1 mmol of sodium heptanesulfonate per liter. 2-Phenylimidazole is the internal standard. The detection limit is 1 microgram/L for nicotine and 3 micrograms/L for cotinine. The standard curve is linear from 0 to 700 micrograms/L for both compounds. The average CV for nicotine in the concentration range 0-100 micrograms/L is 6.5%, and that for cotinine in the concentration range 50-700 micrograms/L is 4%.

scholarly journals Simultaneous determination of ethinyl estradiol and drospirenone in oral contraceptive by high performance liquid chromatography

2013 ◽  
Vol 49 (3) ◽  
pp. 521-528 ◽  
Author(s):  
Viviane Benevenuti Silva ◽  
Angel Arturo Gaona Galdos ◽  
Cintia Maria Alves Mothe ◽  
Michele Bacchi Pallastrelli ◽  
Maria Segunda Aurora Prado ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
High Performance ◽  
Ethinyl Estradiol ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Elution Time ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

A simple, rapid, economical and reliable high performance liquid chromatographic method has been developed and successfully applied in simultaneous determination of ethinyl estradiol and drospirenone in coated tablets. The HPLC method was performed on a LiChroCART® 100RP column (125x4 mm i.d., 5 µm) with acetonitrile:water 50:50 (v/v) as mobile phase, pumped at a flow rate of 1.0 mL.min-1. The fluorescence detection for ethinyl estradiol was made at λex= 280 nm and λem= 310 nm and a UV detection for drospirenone was made at 200 nm. The elution time for ethinyl estradiol and drospirenone were 4.0 and 5.7 min, respectively. The method was validated in accordance to USP 34 guidelines. The proposed HPLC method presented advantages over reported methods and is suitable for quality control assays of ethinyl estradiol and drospirenone in coated tablets.

scholarly journals High performance liquid chromatographic method for the determination of ambroxol hydrochloride in presence of antimicrobial preservatives in oral liquid formulation

2015 ◽  
Vol 18 (1) ◽  
pp. 8-14 ◽  
Author(s):  
T Sudha ◽  
K Manthena ◽  
VR Ravikumar ◽  
V Ganesan
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Methyl Paraben ◽  
Propyl Paraben ◽  
Liquid Chromatographic Method ◽  
Oral Liquid ◽  
Ambroxol Hydrochloride ◽  
Liquid Chromatographic

A simple gradient reversed phase high performance liquid chromatographic method was developed for the determination of ambroxol hydrochloride in presence of antimicrobial preservatives in oral liquid formulation. The chromatographic separation was achieved by an Inertsil C8 (250 X 4.6 mm, 5? particle size) column using gradient technique. The eluents were detected at 245 nm with photodiode array detector. The optimized mobile phase consisted of 0.1% trifluoroacetic acid as a mobile phase A and a mixture of mobile phase A and acetonitrile in the ratio of 76:24 % v/v as mobile phase B. Ambroxol hydrochloride and microbial preservatives were eluted at a flow rate of 1.0 ml/min. The method validated according to the International Conference of Harmonization (ICH) guidelines. The calibration curves were linear over the (r2 > 0.99) concentrations range from 300 to 900 ppm for ambroxol hydrochloride, 10 to 30 ppm for propyl paraben and 100 to 300 ppm for methyl paraben. The limit of detection was found to be 0.024 ppm for ambroxol hydrochloride, 0.018 ppm for propyl paraben and 0.009 ppm for methyl paraben. The percentage recoveries were found to be in the range from 99.55 to 101.1% for ambroxol hydrochloride, 100.31 to 101.46% for propyl paraben and 98.18 to 101.61% for methyl paraben. Stability indicating capability was established by forced degradation experiments. No chromatographic interference from the degradation products was found. The proposed method was highly sensitive, precision and accurate and hence successfully applied for the quantification of ambroxol active pharmaceutical ingredients (API) and preservatives content in the commercially available oral liquid formulation.Bangladesh Pharmaceutical Journal 18(1): 8-14, 2015

scholarly journals High-Performance Liquid Chromatographic Determination of Proquazone and Its m-Hydroxy Metabolite in Spiked Human Plasma and Urine

2007 ◽  
Vol 90 (4) ◽  
pp. 971-976
Author(s):  
Ekram M Hassan ◽  
Azza A Gazy ◽  
Mohamed H Abdel-Hay ◽  
Tarek S Belal
Keyword(s):  
Human Plasma ◽  
Mobile Phase ◽  
High Performance ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Urine Samples ◽  
Spiked Human Plasma ◽  
Liquid Chromatographic ◽  
Hydroxy Metabolite

Abstract A simple and rapid high-performance liquid chromatographic method for the determination of proquazone (PQZ) and its major metabolite, m-hydroxyproquazone, in spiked human plasma and urine was developed. Plasma samples were purified using acetonitrile as a protein precipitant, while urine samples were diluted only with the mobile phase and filtered prior to injection. Samples containing the parent compounds and glafenine (internal standard) were eluted from a reversed-phase C8 column using acetonitrile-0.025 M sodium acetate (60 + 40) adjusted to pH 5 as the mobile phase and detected at 234 nm. Peak area ratios of the analytes versus internal standard were used for calibration. The mean recoveries from plasma and urine samples spiked with PQZ and its m-hydroxy metabolite ranged from 97.87 to 103.88%. The relative standard deviation for the within- and between-day analyses were <4%. The proposed method was applied for the assay of PQZ in laboratory-made tablets.

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