A simple silver-staining technique for detecting Bence Jones proteins in unconcentrated urine.

1987 ◽  
Vol 33 (4) ◽  
pp. 561-563 ◽  
Author(s):  
J M Sheat ◽  
M A Lorier
Keyword(s):  
Silver Staining ◽  
Clinical Laboratory ◽  
Staining Technique ◽  
Silver Stain ◽  
Concentration Step ◽  
Silver Staining Technique ◽  
Bence Jones Proteins

Abstract Detection of Bence Jones proteins in urine usually involves a concentration step, followed by electrophoresis and, if necessary, immunofixation. The time-consuming and expensive concentration step can be eliminated by use of the silver-stain technique described here. This procedure, routinely used for staining unconcentrated urine, is inexpensive, sensitive, and easily performed in a clinical laboratory. Bence Jones proteins can be detected in concentrations as low as 5 mg/L.

scholarly journals Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR).

1993 ◽  
Vol 41 (3) ◽  
pp. 439-445 ◽  
Author(s):  
L E Lindner
Keyword(s):  
Silver Staining ◽  
Nucleolar Organizer ◽  
Staining Technique ◽  
Nucleolar Organizer Regions ◽  
Silver Stain ◽  
Oxidation Reduction ◽  
Background Staining ◽  
Silver Staining Technique ◽  
Selection Of ◽  
Nucleolar Function

The silver-staining technique for nucleolar organizer regions (AgNOR) of Ploton et al., as popularized by Crocker, is being widely used for evaluation of nucleolar function, especially in neoplasia. It suffers from limited reliability, background staining, precipitates, and fading of the sections. Factors were identified that affect these problems. The oxidation-reduction level and gelatin used are particularly important. An improved procedure is presented which incorporates pre-reduction of the sections, selection of an optimal gelatin, and post-treatment of the sections to produce a permanent preparation. It is compatible with many fixatives and with other stains used before or after the silver stain.

New approaches to the role of sulfhydryl groups in silver stainability of protein in grasshopper chromosomes

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 133-137 ◽  
Author(s):  
J. de la Torre ◽  
J. L. Bella ◽  
C. López-Fernandez ◽  
J. Gosálvez
Keyword(s):  
Silver Staining ◽  
Chemical Nature ◽  
Germ Line ◽  
Staining Technique ◽  
Sulfhydryl Groups ◽  
Sh Groups ◽  
New Approaches ◽  
Silver Staining Technique ◽  
Reduced State

Silver staining in somatic and germ line cells of the grasshopper Arcyptera fusca has been analyzed after a standard silver staining technique was used with pretreatments that included dithiothreitol and β-mercaptoethanol as reagents to maintain —SH groups in the reduced state. The results show that in some tissues, these pretreatments improve not only the silver staining of nucleolar masses but also the recognition of other silver spots associated with the chromatin. These observations are similar to those previously described for the effect of double-strength standard saline citrate on silver staining. The possible chemical nature of the protein groups responsible for the differential silver stainability and the role of saline citrate in the modification of argyrophylic proteins suggested previously are briefly discussed.Key words: Orthoptera, silver staining, sulfhydryl groups.

A silver-staining technique for detecting minute quantities of proteins on nitrocellulose paper: Retention of antigenicity of stained proteins

1982 ◽  
Vol 126 (2) ◽  
pp. 398-402 ◽  
Author(s):  
K.C.Leonard Yuen ◽  
Terrell K. Johnson ◽  
Robin E. Denell ◽  
Richard A. Consigli
Keyword(s):  
Silver Staining ◽  
Staining Technique ◽  
Silver Staining Technique
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