Concanavalin A-bound selenoprotein in human serum analyzed by graphite furnace atomic absorption spectrometry

1994 ◽  
Vol 40 (1) ◽  
pp. 62-70 ◽  
Author(s):  
R Daher ◽  
F Van Lente
Keyword(s):  
Human Serum ◽  
Atomic Absorption ◽  
Concanavalin A ◽  
Graphite Furnace ◽  
Direct Determination ◽  
Absorption Spectrometry ◽  
Normal Subjects ◽  
Acute Phase Reactant ◽  
Single Chain ◽  
Total Selenium

Abstract We developed an assay for the direct determination of selenium in serum with a Perkin-Elmer Model 4100ZL Zeeman atomic absorption spectrometer and Ag-Cu-Mg modifier. We used this assay to analyze concanavalin A-bound selenoprotein (CABSP) in human serum after concanavalin A (ConA) affinity chromatography. The CABSP was identified as a single-chain glycoprotein of 57.3 kDa. Carbohydrate units were N- and O-linked to the protein. The selenium moiety was selenocysteine. Total selenium, glutathione peroxidase (GPX; EC 1.11.1.9), ConA-bound selenium (CABS), and alpha 1-acid glycoprotein (AAG) were determined in normal subjects and patients with various pathological conditions. CABS accounted for 44.1% +/- 6.3% of total selenium in sera from normal subjects and 46.5% +/- 3.9% to 55.1% +/- 8.1% in sera from patients with a variety of diseases. Total selenium in serum was well correlated with serum CABS (r = 0.860), but not with serum GPX activity (r = 0.117), for all patients studied. Serum CABS increased in normal subjects after selenium supplementation. Serum CABSP did not behave as an acute-phase reactant, compared with AAG.

scholarly journals Determination of Lead in Croatian Wines by Graphite Furnace Atomic Absorption Spectrometry

2011 ◽  
Vol 62 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Blanka Tariba ◽  
Alica Pizent ◽  
Zorana Kljaković-Gašpić
Keyword(s):  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Graphite Furnace ◽  
Accurate Determination ◽  
Direct Determination ◽  
Absorption Spectrometry ◽  
White Wine ◽  
Matrix Modifier ◽  
Graphite Furnace Atomic Absorption

Determination of Lead in Croatian Wines by Graphite Furnace Atomic Absorption SpectrometryA method has been developed for direct determination of lead in wine by graphite furnace atomic absorption spectrometry (GFAAS) with Zeeman-effect background correction. The thermal behaviour of Pb during pyrolysis and atomisation stages was investigated without matrix modifier and in the presence of Pd(NO3)2, Pd(NO3)2+ Mg(NO3)2× 6H2O, and NH4H2PO4+ Mg(NO3)2× 6H2O as matrix modifiers. A simple 1:1 dilution of wine samples with Pd(NO3)2as a matrix modifier proved optimal for accurate determination of Pb in wine. Mean recoveries were 106 % for red and 114 % for white wine, and the detection limit was 3 μg L-1. Within-run precision of measurements for red and white wine was 2.1 % and 1.8 %, respectively. The proposed method was applied for analysis of 23 Croatian wines. Median Pb concentrations were 33 μg L-1, range (16 to 49) μg L-1in commercially available wines and 46 μg L-1, range (14 to 559) μg L-1in home-made wines. There were no statistically significant differences (P<0.05) in Pb concentration between commercial and home-made wines or between red and white wines.

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