Preparation of a Prototype Inactivated Hepatitis A Virus Vaccine from Infected Cell Cultures

1986 ◽  
Vol 153 (4) ◽  
pp. 749-756 ◽  
Author(s):  
L. N. Binn ◽  
W. H. Bancroft ◽  
S. M. Lemon ◽  
R. H. Marchwicki ◽  
J. W. LeDuc ◽  
...  
Keyword(s):  
Infected Cell ◽  
Cell Cultures ◽  
Virus Vaccine ◽  
Hepatitis A ◽  
Hepatitis A Virus

Identification of hepatitis a virus in cell cultures by solid phase immune electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 238-239
Author(s):  
C.D. Humphrey ◽  
T.L. Cromeans ◽  
E.H. Cook ◽  
D.W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Liquid Phase ◽  
Cell Cultures ◽  
Rapid Detection ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immune Electron Microscopy ◽  
Detection And Identification

There is a variety of methods available for the rapid detection and identification of viruses by electron microscopy as described in several reviews. The predominant techniques are classified as direct electron microscopy (DEM), immune electron microscopy (IEM), liquid phase immune electron microscopy (LPIEM) and solid phase immune electron microscopy (SPIEM). Each technique has inherent strengths and weaknesses. However, in recent years, the most progress for identifying viruses has been realized by the utilization of SPIEM.

Detection of Hepatitis a Virus and other Enteroviruses in Wastewater and Surface Water Samples by Gene Probe Assay

1991 ◽  
Vol 24 (2) ◽  
pp. 267-272 ◽  
Author(s):  
S. Dubrou ◽  
H. Kopecka ◽  
J. M. Lopez Pila ◽  
J. Maréchal ◽  
J. Prévot
Keyword(s):  
Surface Water ◽  
Cell Cultures ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Limit Of Detection ◽  
Blot Hybridization ◽  
Noncoding Region ◽  
Gene Probe ◽  
Dot Blot ◽  
Lower Efficiency

Enteroviruses were specifically detected by dot blot hybridization when using poliovirus type 1 (PV1) derived subgenomic radiolabeled cRNA probes (riboprobes) in environmental water specimens and in the cell cultures in which the viruses were amplificated. The riboprobe corresponding to the 5' noncoding sequence detected the majority of enteroviruses. Hepatitis A virus (HAV) was specifically detected by an HAV cRNA probe corresponding to the 5' noncoding region of its genome. By this test, the limit of detection of coxsackievirus B5 and echovirus 7 seeded in mineral water was 103 to 104 PFU/spot. In cell cultures, positive signals were observed in the lysates of cells infected by one PFU. Higher positive signals were obtained with a short PV1 probe (nt 221-670) corresponding to the 5' noncoding region, which is a well preserved sequence among the enteroviruses, than with PV1 genomic probe. Hybridization allowed a good detection of enteroviral RNAs in wastewater specimens, but with a lower efficiency in surface water. In this case, amplification of viruses in the cell cultures gave significant hybridization results.

scholarly journals Responses to Hepatitis A Virus Vaccine in HIV-Infected Women

2012 ◽  
Vol 60 (1) ◽  
pp. e15-e18 ◽  
Author(s):  
Adriana Weinberg ◽  
Amanda A. Allshouse ◽  
Samantha MaWhinney ◽  
Jennifer Canniff ◽  
Lorie Benning ◽  
...  
Keyword(s):  
Virus Vaccine ◽  
Hepatitis A ◽  
Hepatitis A Virus

Immunogenicity of Hepatitis A Virus Vaccine in Primates

1990 ◽  
Vol 161 (3) ◽  
pp. 586-587 ◽  
Author(s):  
R. K. Chaudhary ◽  
T. Mo
Keyword(s):  
Virus Vaccine ◽  
Hepatitis A ◽  
Hepatitis A Virus

Safety and Immunogenicity of a Live Attenuated Hepatitis A Virus Vaccine in Seronegative Volunteers

1991 ◽  
Vol 163 (4) ◽  
pp. 735-739 ◽  
Author(s):  
K. Midthun ◽  
E. Ellerbeck ◽  
K. Gershman ◽  
G. Calandra ◽  
D. Krah ◽  
...  
Keyword(s):  
Virus Vaccine ◽  
Hepatitis A ◽  
Hepatitis A Virus

scholarly journals Long-Term Immunogenicity of Hepatitis A Virus Vaccine in Alaska 17 Years After Initial Childhood Series

2012 ◽  
Vol 207 (3) ◽  
pp. 493-496 ◽  
Author(s):  
Gregory A. Raczniak ◽  
Lisa R. Bulkow ◽  
Michael G. Bruce ◽  
Carolyn L. Zanis ◽  
Richard L. Baum ◽  
...  
Keyword(s):  
Virus Vaccine ◽  
Hepatitis A ◽  
Hepatitis A Virus
Sign in / Sign up

Export Citation Format

Share Document