A Comparison of Human and Bovine Escherichia coli O157:H7 Isolates by Toxin Genotype, Plasmid Profile, and Bacteriophage  -Restriction Fragment Length Polymorphism Profile

1993 ◽  
Vol 168 (5) ◽  
pp. 1300-1303 ◽  
Author(s):  
M. Paros ◽  
P. I. Tarr ◽  
H. Kim ◽  
T. E. Besser ◽  
D. D. Hancock
Keyword(s):  
Escherichia Coli ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Plasmid Profile ◽  
Escherichia Coli O157 ◽  
Restriction Fragment Length

Molecular typing of Neisseria gonorrhoeae from hospital and community isolates by restriction fragment length polymorphism

2002 ◽  
Vol 13 (2) ◽  
pp. 124-130 ◽  
Author(s):  
M P Bindu ◽  
P Rajendran ◽  
S P Thyagarajan ◽  
N P Pramod ◽  
Usha Anand Rao ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Neisseria Gonorrhoeae ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Antibiotic Sensitivity ◽  
Dna Amplification ◽  
Plasmid Profile ◽  
Restriction Fragment Length

Strains of Neisseria gonorrhoeae are generally characterized by auxotyping, serotyping, plasmid profile, antibiotic sensitivity and deoxyribonucleic acid (DNA) amplification fingerprinting. The aim of this study was to analyse the generation of restriction fragment length polymorphism (RFLP) patterns by BgIII digestion of total genomic DNA of N. gonorrhoeae isolated from the community ( n =30) and the hospital ( n =15) and to establish an association with serogrouping and antibiogram. The RFLP patterns produced by BgIII restriction digestion showed 7 different patterns among 30 community isolates and 9 different patterns among 15 hospital isolates. 66.7% of isolates belonged to serogroup WI. Penicillin resistance was observed in 46.7% of community isolates and 66.7% hospital isolates. However, penicillinase producing N. gonorrhoeae (PPNG) were lower in the community (6.6%) than in the hospital isolates (53.3%). PPNG strains were more often seen in serogroup WI. This is the first Indian report on RFLP genotype pattern in N. gonorrhoeae. We noted differences in RFLP genotypes of the community (RFLP types 1, 2, 3, 4, 5 and 7) and hospital strains (RFLP types 6 and 8), while no differences in the serogroup were observed. Ciprofloxacin resistance was 20.0% and 26.6% in the community and hospital isolates, respectively. Ceftriaxone emerges as the current drug of choice for an effective policy of antibiotic treatment of gonorrhoea through syndromic management in developing countries.

scholarly journals Genetic Typing of Shiga Toxin 2 Variants of Escherichia coli by PCR-Restriction Fragment Length Polymorphism Analysis

2004 ◽  
Vol 70 (10) ◽  
pp. 6309-6314 ◽  
Author(s):  
Liesbet De Baets ◽  
Imme Van der Taelen ◽  
Marina De Filette ◽  
Denis Piérard ◽  
Lesley Allison ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Shiga Toxin ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Polymorphism Analysis ◽  
Shiga Toxin 2 ◽  
Restriction Fragment Length

ABSTRACT Shiga toxins Stx1 and Stx2 play a prominent role in the pathogenesis of Shiga toxin-producing Escherichia coli (STEC) infections. Several variants of the stx 2 gene, encoding Stx2, have been described. In this study, we developed a PCR-restriction fragment length polymorphism system for typing stx 2 genes of STEC strains. The typing system discriminates eight described variants and allows the identification of new stx 2 variants and STEC isolates carrying multiple stx 2 genes. A phylogenetic tree, based on the nucleotide sequences of the toxin-encoding genes, demonstrates that stx 2 sequences with the same PvuII HaeIII HincII AccI type generally cluster together.

scholarly journals Comparison of serotype, biotype and bacteriocin type with rDNA RFLP patterns for the type identification ofSerratia marcescens

1993 ◽  
Vol 111 (1) ◽  
pp. 99-107 ◽  
Author(s):  
R. Alonso ◽  
H. M. Aucken ◽  
J. C. Perez-Diaz ◽  
B. D. Cookson ◽  
F. Baquero ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Restriction Fragment Length Polymorphism ◽  
Serratia Marcescens ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Rdna Gene ◽  
Bacteriocin Typing ◽  
Restriction Fragment Length

SummaryVariations in rDNA gene loci in DNA digests of 209 clinical isolates ofSerratia marcescenswere determined with anEscherichia colirRNA probe. Forty-one restriction fragment length polymorphism patterns (ribotypes) were identified, based on the size of 4–14 (mean 7·5) hybridization bands. The patterns differed by more than a single band in 98% of pair-wise comparisons. On a subset of 76 isolates, ribotyping proved to be marginally more discriminating than biotyping (discrimination index 0·92v. 0·89) followed by serotyping (0·87) and bacteriocin typing (0·74). About one-third of isolates belonged to unique ribotypes and only two ribotypes exceeded 5% in frequency (23·0 and 6·4% respectively). A combination of serotype or biotype with ribotyping defined a similar number of strains, although none of the methods alone was sufficiently discriminatory to identify strains. We conclude that due to the accessibility of biotyping and the lack of commercially available antisera forS. marcescens, the biotype and ribotype together provide reliable markers of strain identity.

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