Dried Blood Spots on Filter Paper as an Alternative Specimen for Measles Diagnostics: Detection of Measles Immunoglobulin M Antibody by a Commercial Enzyme Immunoassay

ABSTRACT Most persons with rubella virus-specific immunoglobulin M (IgM)- or IgG-positive sera tested positive (98% [n = 178] and 99% [n = 221], respectively) using paired filter paper dried blood spot (DBS) samples, provided that DBS indeterminate results were called positive. For persons with IgM- or IgG-negative sera, 97% and 98%, respectively, were negative using DBS.

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An Enzyme Immunoassay for Determining Immunoreactive Trypsinogen (IRT) in Dried Blood Spots on Filter Paper Using an Ultra-Microanalytical System

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-018-2785-4 ◽

2018 ◽

Vol 186 (4) ◽

pp. 1034-1046 ◽

Cited By ~ 2

Author(s):

Elisa M. Castells Martínez ◽

Ernesto Carlos González ◽

Yileidis Tejeda ◽

Amarilys Frómeta ◽

Odalys Martín ◽

...

Keyword(s):

Enzyme Immunoassay ◽

Filter Paper ◽

Dried Blood Spots ◽

Blood Spots ◽

Immunoreactive Trypsinogen

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An enzyme immunoassay for determining 17α-hydroxyprogesterone in dried blood spots on filter paper using an ultramicroanalytical system

Clinica Chimica Acta ◽

10.1016/j.cca.2008.04.004 ◽

2008 ◽

Vol 394 (1-2) ◽

pp. 63-66 ◽

Cited By ~ 10

Author(s):

Ernesto Carlos González ◽

Neivis Marrero ◽

Pedro Lucio Pérez ◽

Amarilys Frómeta ◽

Orlando Zulueta ◽

...

Keyword(s):

Enzyme Immunoassay ◽

Filter Paper ◽

Dried Blood Spots ◽

Blood Spots

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Comparison between Enzyme Immunoassays Performed on Samples of Dried Blood and Serum for Toxoplasmosis Prenatal Screening: Population-based Study

Revista Brasileira de Ginecologia e Obstetrícia / RBGO Gynecology and Obstetrics ◽

10.1055/s-0041-1730285 ◽

2021 ◽

Vol 43 (05) ◽

pp. 351-356

Author(s):

Bárbara Araújo Marques ◽

Ericka Vianna Machado Carellos ◽

Vânia Maria Novato Silva ◽

Fernando Henrique Pereira ◽

Maria Regina Lage Guerra ◽

...

Keyword(s):

Pregnant Women ◽

Prenatal Screening ◽

Dried Blood Spots ◽

Population Based ◽

Minas Gerais ◽

Immunoglobulin M ◽

Screening Tests ◽

Reference Test ◽

Screening Programs ◽

Blood Spots

Abstract Objective Most prenatal screening programs for toxoplasmosis use immunoassays in serum samples of pregnant women. Few studies assess the accuracy of screening tests in dried blood spots, which are of easy collection, storage, and transportation. The goals of the present study are to determine the performance and evaluate the agreement between an immunoassay of dried blood spots and a reference test in the serum of pregnant women from a population-based prenatal screening program for toxoplasmosis in Brazil. Methods A cross-sectional study was performed to compare the immunoassays Imunoscreen Toxoplasmose IgM and Imunoscreen Toxoplasmose IgG (Mbiolog Diagnósticos, Ltda., Contagem, Minas Gerais, Brazil)in dried blood spots with the enzyme-linked fluorescent assay (ELFA, BioMérieux S.A., Lyon, France) reference standard in the serum of pregnant women from Minas Gerais Congenital Toxoplasmosis Control Program. Results The dried blood spot test was able to discriminate positive and negative results of pregnant women when compared with the reference test, with an accuracy of 98.2% for immunoglobulin G (IgG), and of 95.8% for immunoglobulin M (IgM). Conclusion Dried blood samples are easy to collect, store, and transport, and they have a good performance, making this a promising method for prenatal toxoplasmosis screening programs in countries with continental dimensions, limited resources, and a high prevalence of toxoplasmosis, as is the case of Brazil.

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