Alpha- l -iduronidase and arylsulfatase B in dried blood spots on filter paper: Biochemical parameters and time stability

2017 ◽  
Vol 50 (7-8) ◽  
pp. 431-435 ◽  
Author(s):  
Ana Carolina Breier ◽  
Jaqueline Cé ◽  
Jamila Mezzalira ◽  
Vanessa V. Daitx ◽  
Vitoria C. Moraes ◽  
...  
Keyword(s):  
Filter Paper ◽  
Biochemical Parameters ◽  
Dried Blood Spots ◽  
Time Stability ◽  
Blood Spots ◽  
Arylsulfatase B

Influence of pre-analytical factors on α-galactosidase A, arylsulfatase B and α-glucosidase activities measured on dried blood spots on filter paper

2011 ◽  
Vol 44 (10-11) ◽  
pp. 922-926 ◽  
Author(s):  
Cristina D. de Castilhos ◽  
Jamila Mezzalira ◽  
Mariana P.S. Goldim ◽  
Janice C. Coelho
Keyword(s):  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Arylsulfatase B

PCR of DNA from dried blood spots on filter paper coupled to a simple DNA enzyme immunoassay for rapid detection of HIV-1

1996 ◽  
Vol 52 (4) ◽  
pp. 308-309
Author(s):  
B. Schweiger ◽  
C. Kücherer ◽  
C. Fleischer ◽  
H. v. Spreckelsen ◽  
P. Zablocki-Kaiser ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Rapid Detection ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Dna Enzyme Immunoassay ◽  
Hiv 1

Determination of Chloroquine in Dried Blood Spots on Filter Paper

1986 ◽  
Vol 8 (2) ◽  
pp. 211-213 ◽  
Author(s):  
Y. Bergqvist ◽  
Ö. Ericsson ◽  
M. Rais
Keyword(s):  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Spots

Fluorometric micromethod for determination of arginase activity in dried blood spots on filter paper.

1980 ◽  
Vol 26 (8) ◽  
pp. 1198-1200 ◽  
Author(s):  
A P Orfanos ◽  
E W Naylor ◽  
R Guthrie
Keyword(s):  
Filter Paper ◽  
Colorimetric Method ◽  
Dried Blood Spots ◽  
Arginase Activity ◽  
Kinetic Reaction ◽  
Hematological Disorders ◽  
Blood Spots ◽  
Blood Specimens ◽  
The Stability

Abstract We describe a microfluorometric method for determination of arginase activity in dried blood spots on filter paper. The arginase in discs punched from such dried blood specimens is activated by preincubation with Mn2+ at 37 degrees C. After incubation with substrate at the same temperature, urea is determined fluorometrically by oxidation of NADH to NAD+ in a coupled kinetic reaction. We compare the results of this method with those of a colorimetric method involving liquid blood samples, and assess the stability of the enzyme in dried blood on filter paper. The presence of serum has no effect on the activity. This method may be useful in the early detection of arginase deficiency and certain hematological disorders.

Measurement of gonadotropins in dried blood spots

1994 ◽  
Vol 40 (3) ◽  
pp. 448-453 ◽  
Author(s):  
C M Worthman ◽  
J F Stallings
Keyword(s):  
Luteinizing Hormone ◽  
Filter Paper ◽  
Clinical Studies ◽  
Follicle Stimulating Hormone ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Low Concentrations ◽  
Serial Sampling ◽  
Highly Correlated ◽  
Blood Spot

Abstract We describe direct immunofluorometric assays for luteinizing hormone (hLH) and follicle-stimulating hormone (hFSH) in fingerstick blood spots dried on filter paper, based on modifications of commercially available kits. Determinations are made from 2.5-mm-diameter discs (3 microL of dried blood) punched out from blood spot standards and samples. Sample dose detection limits of the assays (IU/L) are 0.26 for LH and 0.13 for FSH, with mean interassay CVs of 11.6% (LH) and 7.8% (FSH) at low concentrations. Analytical recoveries of added hormone averaged 100% for LH and 95% for FSH. Clinical studies showed that values for blood spots (x) and directly assayed plasma (y) are highly correlated, so that results from blood spots can be converted directly to plasma equivalents, as follows: yLH = 0.07 + 1.90 xLH, and yFSH = 0.424 + 2.207 xFSH. These gonadotropins are stable in blood spots for at least a year under refrigeration; LH for at least 8 weeks and FSH 6 weeks at 22 degrees C; and both hormones for a week at 37 degrees C. These methods thus allow self-sampling, serial sampling, and mailing of specimens.

Ultramicro-fluorometric assay for the diagnosis of Gaucher disease in dried blood spots on filter paper

2013 ◽  
Vol 6 (1) ◽  
pp. 61-67
Author(s):  
D. Herrera ◽  
M. Monaga ◽  
D. Campos ◽  
Y. Pampín ◽  
E.C. González ◽  
...  
Keyword(s):  
Filter Paper ◽  
Gaucher Disease ◽  
Dried Blood Spots ◽  
Fluorometric Assay ◽  
Blood Spots
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