An Enzyme Immunoassay for Determining Immunoreactive Trypsinogen (IRT) in Dried Blood Spots on Filter Paper Using an Ultra-Microanalytical System

2018 ◽  
Vol 186 (4) ◽  
pp. 1034-1046 ◽  
Author(s):  
Elisa M. Castells Martínez ◽  
Ernesto Carlos González ◽  
Yileidis Tejeda ◽  
Amarilys Frómeta ◽  
Odalys Martín ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Immunoreactive Trypsinogen

PCR of DNA from dried blood spots on filter paper coupled to a simple DNA enzyme immunoassay for rapid detection of HIV-1

1996 ◽  
Vol 52 (4) ◽  
pp. 308-309
Author(s):  
B. Schweiger ◽  
C. Kücherer ◽  
C. Fleischer ◽  
H. v. Spreckelsen ◽  
P. Zablocki-Kaiser ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Rapid Detection ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Dna Enzyme Immunoassay ◽  
Hiv 1

An enzyme immunoassay for determining 17α-hydroxyprogesterone in dried blood spots on filter paper using an ultramicroanalytical system

2008 ◽  
Vol 394 (1-2) ◽  
pp. 63-66 ◽  
Author(s):  
Ernesto Carlos González ◽  
Neivis Marrero ◽  
Pedro Lucio Pérez ◽  
Amarilys Frómeta ◽  
Orlando Zulueta ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Spots

Alpha- l -iduronidase and arylsulfatase B in dried blood spots on filter paper: Biochemical parameters and time stability

2017 ◽  
Vol 50 (7-8) ◽  
pp. 431-435 ◽  
Author(s):  
Ana Carolina Breier ◽  
Jaqueline Cé ◽  
Jamila Mezzalira ◽  
Vanessa V. Daitx ◽  
Vitoria C. Moraes ◽  
...  
Keyword(s):  
Filter Paper ◽  
Biochemical Parameters ◽  
Dried Blood Spots ◽  
Time Stability ◽  
Blood Spots ◽  
Arylsulfatase B

Determination of Chloroquine in Dried Blood Spots on Filter Paper

1986 ◽  
Vol 8 (2) ◽  
pp. 211-213 ◽  
Author(s):  
Y. Bergqvist ◽  
Ö. Ericsson ◽  
M. Rais
Keyword(s):  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Spots

Measurement of Theophylline in Dried Blood Spots by Spectrophotometric Enzyme Immunoassay

1988 ◽  
Vol 25 (6) ◽  
pp. 650-653 ◽  
Author(s):  
J M Rattenbury ◽  
Tracy Taylor
Keyword(s):  
Enzyme Immunoassay ◽  
Dried Blood Spots ◽  
Plasma Samples ◽  
Blood Spots

A widely-used enzyme immunoassay for the measurement of theophylline in plasma (EMIT) has been adapted for use with dried blood spots. Potential interference from haemoglobin in eluates of the spots was avoided by autoclaving them prior to analysis. The method was investigated in terms of the recovery of theophylline added to samples, precision, and the correlation of results in DBS with those in plasma samples. The recoveries of some other drugs from eluates of autoclaved DBS were also investigated.

Fluorometric micromethod for determination of arginase activity in dried blood spots on filter paper.

1980 ◽  
Vol 26 (8) ◽  
pp. 1198-1200 ◽  
Author(s):  
A P Orfanos ◽  
E W Naylor ◽  
R Guthrie
Keyword(s):  
Filter Paper ◽  
Colorimetric Method ◽  
Dried Blood Spots ◽  
Arginase Activity ◽  
Kinetic Reaction ◽  
Hematological Disorders ◽  
Blood Spots ◽  
Blood Specimens ◽  
The Stability

Abstract We describe a microfluorometric method for determination of arginase activity in dried blood spots on filter paper. The arginase in discs punched from such dried blood specimens is activated by preincubation with Mn2+ at 37 degrees C. After incubation with substrate at the same temperature, urea is determined fluorometrically by oxidation of NADH to NAD+ in a coupled kinetic reaction. We compare the results of this method with those of a colorimetric method involving liquid blood samples, and assess the stability of the enzyme in dried blood on filter paper. The presence of serum has no effect on the activity. This method may be useful in the early detection of arginase deficiency and certain hematological disorders.

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