Simple Method for Determination of Glycine and Niacin in Drug Mixtures

1968 ◽  
Vol 51 (1) ◽  
pp. 11-14
Author(s):  
Keith L Egli ◽  
Anthony Romano
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ultraviolet Absorption ◽  
Simple Method ◽  
Drug Mixtures ◽  
Layer Chromatography

Abstract A method, based on the formol titration of glycine and the ultraviolet absorption of niacin, has been developed for the determination of these compounds in drug mixtures. Neither compound requires isolation for the determination. Glycine and niacin are identified by thin layer chromatography

Determination of Aflatoxins in Vegetable Oils

1985 ◽  
Vol 68 (1) ◽  
pp. 136-137
Author(s):  
Noah Miller ◽  
Helena E Pretorius ◽  
Donald W Trinder
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Vegetable Oils ◽  
Simple Method ◽  
Silica Column ◽  
Coefficients Of Variation ◽  
Layer Chromatography ◽  
Chloroform Methanol

Abstract A simple method is proposed for determination of aflatoxins in vegetable oils. The method was successfully applied to both crude and degummed oils. The oil sample, dissolved in hexane, was applied to a silica column and washed with ether, toluene, and chloroform; aflatoxins were eluted from the column with chloroform-methanol (97 + 3). As quantitated by thin layer chromatography and liquid chromatography, the oils analyzed contained aflatoxin Bx at levels of 5-200 αg/kg. Recoveries of aflatoxin Bi standards added to aflatoxin-free oils were between 89.5 and 93.5%, with coefficients of variation of 6.3- 8.0%.

scholarly journals Development and Validation of a Method for Determination of Caffeine in Diuretic Tablets and Capsules by High-Performance Thin-Layer Chromatography on Silica Gel Plates with a Concentration Zone Using Manual Spotting and Ultraviolet Absorption Densitometry

2005 ◽  
Vol 88 (5) ◽  
pp. 1537-1543 ◽  
Author(s):  
Caitlin Sullivan ◽  
Joseph Sherma
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
High Performance ◽  
Limit Of Detection ◽  
Pharmaceutical Preparations ◽  
Ultraviolet Absorption ◽  
Concentration Zone ◽  
Layer Chromatography

Abstract A new quantitative method using silica gel high-performance thin-layer chromatography plates with channels and a concentration zone, manual application of standards and samples, development with methanol–ethyl acetate (15 + 85) mobile phase, and ultraviolet absorption densitometry is reported for the determination of caffeine in diuretic pharmaceutical preparations. Tablet and capsule products containing potassium salicylate, acetaminophen, and salicylamide as active ingredients were analyzed to test the applicability of the new method, and precision, accuracy, linearity, limits of detection and quantitation, and selectivity were validated. The milligrams of caffeine in each tablet ranged from 48.0 to 51.0, and the milligrams in each capsule from 37.9 to 40.3. Within-day precision was 1.48 and 1.78% (n = 6), and interday precision 0.723 and 1.26% (n = 5) for analysis of 2 tablets and 2 capsules, respectively. Accuracy validation of the tablet and capsule results produced errors of 1.0 and 1.9% for spiked blank analyses and 2.6 and 3.5% for standard addition analyses, respectively. A comparative study using a caffeine standard solution and a multicomponent analgesic tablet solution containing caffeine, acetaminophen, and acetylsalicylic acid showed that manual application on the concentration zone, instrumental application on the concentration zone, and instrumental application on the silica gel gave quite similar results in terms of number of theoretical plates, resolution, limit of detection, and linearity.

Determination of Quinomethionate (6-Methylquinoline-2,3-diyldithiocarbonate) Residues in Crops by In Situ Fluorometry

1976 ◽  
Vol 59 (1) ◽  
pp. 172-173
Author(s):  
Yves Francoeur ◽  
Victorin Mallet
Keyword(s):  
Thin Layer ◽  
Quantitative Determination ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Pesticide Residue ◽  
Simple Method ◽  
Layer Chromatography ◽  
Ppm Level

Abstract A simple method is described for the quantitative determination of quinomethionate (6-methylquinoline-2,3-diyldithiocarbonate) in crops. The pesticide residue is extracted with acetonitrile and partitioned in petroleum ether. After separation from the co-extractives by thin layer chromatography (TLC), the fluorescence is measured directly on a silica gel TLC plate. An average of 89% recovery is obtained at the 0.05 ppm level in apples, peaches, pears, and tomatoes.

Determination of Functionality Distributions in Telechelic Prepolymers by Thin-Layer Chromatography

1977 ◽  
Vol 50 (1) ◽  
pp. 63-70 ◽  
Author(s):  
T. I. Min ◽  
T. Miyamoto ◽  
H. Inagaki
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Complete Separation ◽  
Hydroxyl Groups ◽  
Simple Method ◽  
Flame Ionisation Detector ◽  
Layer Chromatography ◽  
Flame Ionisation ◽  
Ionisation Detector

Abstract Functionality distributions of telechelic prepolymers have been determined by thin layer chromatography (TLC). Commercially available 1,2-polybutadienes having either carboxyl or hydroxyl groups were examined. TLC with p-xylene as the developer made it possible to separate the sample into a nonfunctional component and a mixture of mono- and difunctional components. Complete separation of the sample into the three components was achieved by selecting the developer and development procedure appropriately. Quantification of the chromatograms was performed successfully in a TLC apparatus equipped with a flame ionisation detector. A simple method for the determination of the functionality distribution was proposed.

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