Molecular characterization of acidic peptide:N-glycanase from the dimorphic yeast Yarrowia lipolytica

2014 ◽  
Vol 157 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Kyung Jin Lee ◽  
Jin Young Gil ◽  
Sang-Yoon Kim ◽  
Ohsuk Kwon ◽  
Kisung Ko ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Yarrowia Lipolytica ◽  
Dimorphic Yeast ◽  
Yeast Yarrowia Lipolytica

Functional characterization of extracellular chitinase encoded by the YlCTS1 gene in a dimorphic yeast Yarrowia lipolytica

2014 ◽  
Vol 52 (4) ◽  
pp. 284-291 ◽  
Author(s):  
Jeong-Nam Park ◽  
Chang Pyo Han ◽  
Dong-Jik Lee ◽  
Seon Ah Cheon ◽  
Hyun Ah Kang
Keyword(s):  
Yarrowia Lipolytica ◽  
Functional Characterization ◽  
Dimorphic Yeast ◽  
Yeast Yarrowia Lipolytica

Characterization of a nontoxic pyomelanin pigment produced by the yeast Yarrowia lipolytica

2020 ◽  
Vol 36 (2) ◽  
Author(s):  
Imen Ben Tahar ◽  
Małgorzata Kus‐Liśkiewicz ◽  
Yannick Lara ◽  
Emmanuelle Javaux ◽  
Patrick Fickers
Keyword(s):  
Yarrowia Lipolytica ◽  
Yeast Yarrowia Lipolytica

The MAT A locus of the dimorphic yeast Yarrowia lipolytica consists of two divergently oriented genes

1999 ◽  
Vol 262 (1) ◽  
pp. 180-188 ◽  
Author(s):  
C. Kurischko ◽  
M. B. Schilhabel ◽  
I. Kunze ◽  
E. Franzl
Keyword(s):  
Yarrowia Lipolytica ◽  
Dimorphic Yeast ◽  
Yeast Yarrowia Lipolytica

scholarly journals Purification and partial characterization of a fatty acidbinding protein from the yeast, Yarrowia lipolytica

IUBMB Life ◽  
1996 ◽  
Vol 39 (3) ◽  
pp. 439-445 ◽  
Author(s):  
Esteban Dell'Angelica ◽  
Mario Ermácora ◽  
José Santomé
Keyword(s):  
Yarrowia Lipolytica ◽  
Partial Characterization ◽  
Yeast Yarrowia Lipolytica

scholarly journals Functional overexpression and characterization of lipogenesis-related genes in the oleaginous yeast Yarrowia lipolytica

2016 ◽  
Vol 100 (8) ◽  
pp. 3781-3798 ◽  
Author(s):  
Andrew M. Silverman ◽  
Kangjian Qiao ◽  
Peng Xu ◽  
Gregory Stephanopoulos
Keyword(s):  
Yarrowia Lipolytica ◽  
Oleaginous Yeast ◽  
Yeast Yarrowia Lipolytica

The dimorphic yeast Yarrowia lipolytica possesses an atypical phosphofructokinase: characterization of the enzyme and its encoding gene

Microbiology ◽  
2005 ◽  
Vol 151 (5) ◽  
pp. 1465-1474 ◽  
Author(s):  
Carmen-Lisset Flores ◽  
Oscar H. Martínez-Costa ◽  
Valentina Sánchez ◽  
Carlos Gancedo ◽  
Juan J. Aragón
Keyword(s):  
Yarrowia Lipolytica ◽  
Carbon Sources ◽  
Hill Coefficient ◽  
Single Type ◽  
Kinetic Characteristics ◽  
Gene Encoding ◽  
Dimorphic Yeast ◽  
Encoding Gene ◽  
Cooperative Kinetics

The phosphofructokinase from the non-conventional yeast Yarrowia lipolytica (YlPfk) was purified to homogeneity, and its encoding gene isolated. YlPfk is an octamer of 869 kDa composed of a single type of subunit, and shows atypical kinetic characteristics. It did not exhibit cooperative kinetics for fructose 6-phosphate (Hill coefficient, h 1·1; S 0·5 52 μM), it was inhibited moderately by MgATP (K i 3·5 mM), and it was strongly inhibited by phosphoenolpyruvate (K i 61 μM). Fructose 2,6-bisphosphate did not activate the enzyme, and AMP and ADP were also without effect. The gene YlPFK1 has no introns, and encodes a putative protein of 953 aa, with a molecular mass consistent with the subunit size found after purification. Disruption of the gene abolished growth in glucose and Pfk activity, while reintroduction of the gene restored both properties. This indicates that Y. lipolytica has only one gene encoding Pfk, and supports the finding that the enzyme consists of identical subunits. Glucose did not interfere with growth of the Ylpfk1 disruptant in permissive carbon sources. The unusual kinetic characteristics of YlPfk, and the intracellular concentrations of glycolytic intermediates during growth in glucose, suggest that YlPfk may play an important role in the regulation of glucose metabolism in Y. lipolytica, different from the role played by the enzyme in Saccharomyces cerevisiae.

Purification and Characterization of the Recombinant Form of Acyl CoA Oxidase 3 from the Yeast Yarrowia lipolytica

2000 ◽  
Vol 384 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Yi-Shan Luo ◽  
Hui-Jie Wang ◽  
K.V Gopalan ◽  
D.K Srivastava ◽  
Jean-Marc Nicaud ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Recombinant Form ◽  
Purification And Characterization ◽  
Acyl Coa Oxidase ◽  
Yeast Yarrowia Lipolytica
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