Characterization of the promoter, downstream target genes and recognition DNA sequence of Mhy1, a key filamentation-promoting transcription factor in the dimorphic yeast Yarrowia lipolytica

2019 ◽  
Vol 66 (1) ◽  
pp. 245-261 ◽  
Author(s):  
Heng Wu ◽  
Tao Shu ◽  
Yi-Sheng Mao ◽  
Xiang-Dong Gao
Keyword(s):  
Transcription Factor ◽  
Yarrowia Lipolytica ◽  
Dna Sequence ◽  
Target Genes ◽  
Downstream Target ◽  
Dimorphic Yeast ◽  
Yeast Yarrowia Lipolytica

Functional characterization of extracellular chitinase encoded by the YlCTS1 gene in a dimorphic yeast Yarrowia lipolytica

2014 ◽  
Vol 52 (4) ◽  
pp. 284-291 ◽  
Author(s):  
Jeong-Nam Park ◽  
Chang Pyo Han ◽  
Dong-Jik Lee ◽  
Seon Ah Cheon ◽  
Hyun Ah Kang
Keyword(s):  
Yarrowia Lipolytica ◽  
Functional Characterization ◽  
Dimorphic Yeast ◽  
Yeast Yarrowia Lipolytica

scholarly journals Identification of the Transcription Factor Znc1p, which Regulates the Yeast-to-Hypha Transition in the Dimorphic Yeast Yarrowia lipolytica

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e66790 ◽  
Author(s):  
Azul Martinez-Vazquez ◽  
Angelica Gonzalez-Hernandez ◽  
Ángel Domínguez ◽  
Richard Rachubinski ◽  
Meritxell Riquelme ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Yarrowia Lipolytica ◽  
Dimorphic Yeast ◽  
Yeast Yarrowia Lipolytica

Molecular characterization of acidic peptide:N-glycanase from the dimorphic yeast Yarrowia lipolytica

2014 ◽  
Vol 157 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Kyung Jin Lee ◽  
Jin Young Gil ◽  
Sang-Yoon Kim ◽  
Ohsuk Kwon ◽  
Kisung Ko ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Yarrowia Lipolytica ◽  
Dimorphic Yeast ◽  
Yeast Yarrowia Lipolytica

scholarly journals New Insights into the Pleiotropic Drug Resistance Network from Genome-Wide Characterization of the YRR1 Transcription Factor Regulation System

2002 ◽  
Vol 22 (8) ◽  
pp. 2642-2649 ◽  
Author(s):  
Stéphane Le Crom ◽  
Frédéric Devaux ◽  
Philippe Marc ◽  
Xiaoting Zhang ◽  
W. Scott Moye-Rowley ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Transcription Factor ◽  
Binding Site ◽  
Time Course ◽  
Target Genes ◽  
Regulation System ◽  
Dependent Manner ◽  
Pleiotropic Drug Resistance ◽  
Genome Wide

ABSTRACT Yrr1p is a recently described Zn2Cys6 transcription factor involved in the pleiotropic drug resistance (PDR) phenomenon. It is controlled in a Pdr1p-dependent manner and is autoregulated. We describe here a new genome-wide approach to characterization of the set of genes directly regulated by Yrr1p. We found that the time-course production of an artificial chimera protein containing the DNA-binding domain of Yrr1p activated the 15 genes that are also up-regulated by a gain-of-function mutant of Yrr1p. Gel mobility shift assays showed that the promoters of the genes AZR1, FLR1, SNG1, YLL056C, YLR346C, and YPL088W interacted with Yrr1p. The putative consensus Yrr1p binding site deduced from these experiments, (T/A)CCG(C/T)(G/T)(G/T)(A/T)(A/T), is strikingly similar to the PDR element binding site sequence recognized by Pdr1p and Pdr3p. The minor differences between these sequences are consistent with Yrr1p and Pdr1p and Pdr3p having different sets of target genes. According to these data, some target genes are directly regulated by Pdr1p and Pdr3p or by Yrr1p, whereas some genes are indirectly regulated by the activation of Yrr1p. Some genes, such as YOR1, SNQ2, and FLR1, are clearly directly controlled by both classes of transcription factor, suggesting an important role for the corresponding membrane proteins.

Characterization of a nontoxic pyomelanin pigment produced by the yeast Yarrowia lipolytica

2020 ◽  
Vol 36 (2) ◽  
Author(s):  
Imen Ben Tahar ◽  
Małgorzata Kus‐Liśkiewicz ◽  
Yannick Lara ◽  
Emmanuelle Javaux ◽  
Patrick Fickers
Keyword(s):  
Yarrowia Lipolytica ◽  
Yeast Yarrowia Lipolytica

The MAT A locus of the dimorphic yeast Yarrowia lipolytica consists of two divergently oriented genes

1999 ◽  
Vol 262 (1) ◽  
pp. 180-188 ◽  
Author(s):  
C. Kurischko ◽  
M. B. Schilhabel ◽  
I. Kunze ◽  
E. Franzl
Keyword(s):  
Yarrowia Lipolytica ◽  
Dimorphic Yeast ◽  
Yeast Yarrowia Lipolytica

scholarly journals Distinct Posttranscriptional Mechanisms Regulate the Activity of the Zn Finger Transcription Factor Lame duck during Drosophila Myogenesis

2006 ◽  
Vol 26 (4) ◽  
pp. 1414-1423 ◽  
Author(s):  
Hong Duan ◽  
Hanh T. Nguyen
Keyword(s):  
Skeletal Muscle ◽  
Transcription Factor ◽  
Posttranslational Modifications ◽  
Target Genes ◽  
Nuclear Translocation ◽  
Protein Activity ◽  
Downstream Target ◽  
Muscle Formation ◽  
Lame Duck ◽  
Fusion Competent Myoblasts

ABSTRACT Skeletal muscle formation in Drosophila melanogaster requires two types of myoblasts, muscle founders and fusion-competent myoblasts. Lame duck (Lmd), a member of the Gli superfamily of transcription factors, is essential for the specification and differentiation of fusion-competent myoblasts. We report herein that appropriate levels of active Lmd protein are attained by a combination of posttranscriptional mechanisms. We provide evidence that two different regions of the Lmd protein are critical for modulating the balance between its nuclear translocation and its retention within the cytoplasm. Activation of the Lmd protein is also tempered by posttranslational modifications of the protein that do not detectably change its subcellular localization. We further show that overexpression of Lmd protein derivatives that are constitutively nuclear or hyperactive results in severe muscle defects. These findings underscore the importance of regulated Lmd protein activity in maintaining proper activation of downstream target genes, such as Mef2, within fusion-competent myoblasts.

scholarly journals Purification and partial characterization of a fatty acidbinding protein from the yeast, Yarrowia lipolytica

IUBMB Life ◽  
1996 ◽  
Vol 39 (3) ◽  
pp. 439-445 ◽  
Author(s):  
Esteban Dell'Angelica ◽  
Mario Ermácora ◽  
José Santomé
Keyword(s):  
Yarrowia Lipolytica ◽  
Partial Characterization ◽  
Yeast Yarrowia Lipolytica
Sign in / Sign up

Export Citation Format

Share Document