Three-dimensional structure of bovine heart NADH: ubiquinone oxidoreductase (complex I) by electron microscopy of a single negatively stained two-dimensional crystal

Microscopy ◽  
2014 ◽  
Vol 63 (2) ◽  
pp. 167-174 ◽  
Author(s):  
Satoru Shimada ◽  
Kyoko Shinzawa-Itoh ◽  
Satoko Amano ◽  
Yui Akira ◽  
Atsuo Miyazawa ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Complex I ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Two Dimensional ◽  
Three Dimensional Structure ◽  
Ubiquinone Oxidoreductase ◽  
Bovine Heart ◽  
Nadh Ubiquinone Oxidoreductase ◽  
Dimensional Crystal

scholarly journals An analysis of the polypeptide composition of bovine heart mitochondrial NADH-ubiquinone oxidoreductase by two-dimensional polyacrylamide-gel electrophoresis

1979 ◽  
Vol 181 (2) ◽  
pp. 435-443 ◽  
Author(s):  
C Heron ◽  
S Smith ◽  
C I Ragan
Keyword(s):  
Gel Electrophoresis ◽  
Complex I ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Two Dimensional ◽  
Submitochondrial Particles ◽  
Ubiquinone Oxidoreductase ◽  
Bovine Heart ◽  
Nadh Ubiquinone Oxidoreductase

Purified preparations of Complex I (NADH-ubiquinone oxidoreductase) from bovine heart mitochondria may be resolved into 26 polypeptides by two-dimensional analysis combining isoelectric focusing and polyacrylamide-gel electrophoresis in sodium dodecyl sulphate. Similar analyses of the fragments obtained from chaotropic resolution of the enzyme show that each of these fragments contains a distinct and non-overlapping set of polypeptides. Evidence that the polypeptides seen in the intact enzyme are true constituents comes from analyses of immunoprecipitates obtained by allowing Complex I or solubilized submitochondrial particles to react with antisera directed against the whole enzyme and a subfragment of the enzyme.

scholarly journals Three-dimensional structure of (Na+ + K+ )-ATPase revealed by electron microscopy of two-dimensional crystals

FEBS Letters ◽  
1985 ◽  
Vol 190 (1) ◽  
pp. 73-76 ◽  
Author(s):  
Yu.A. Ovchinnikov ◽  
V.V. Demin ◽  
A.N. Barnakov ◽  
A.P. Kuzin ◽  
A.V. Lunev ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Two Dimensional ◽  
Three Dimensional Structure

Three-Dimensional Structure of Cloned T3 Connector Protein at 1.6nm Resolution

1990 ◽  
Vol 48 (1) ◽  
pp. 282-283
Author(s):  
José L. Carrascosa ◽  
José M. Valpuesta ◽  
Hisao Fujisawa
Keyword(s):  
Dna Sequences ◽  
Expression Vector ◽  
Three Dimensional ◽  
Deae Cellulose ◽  
Dna Packaging ◽  
Dimensional Structure ◽  
Two Dimensional ◽  
Freezing And Thawing ◽  
Three Dimensional Structure ◽  
Dimensional Reconstruction

The head to tail connector of bacteriophages plays a fundamental role in the assembly of viral heads and DNA packaging. In spite of the absence of sequence homology, the structure of connectors from different viruses (T4, Ø29, T3, P22, etc) share common morphological features, that are most clearly revealed in their three-dimensional structure. We have studied the three-dimensional reconstruction of the connector protein from phage T3 (gp 8) from tilted view of two dimensional crystals obtained from this protein after cloning and purification.DNA sequences including gene 8 from phage T3 were cloned, into Bam Hl-Eco Rl sites down stream of lambda promotor PL, in the expression vector pNT45 under the control of cI857. E R204 (pNT89) cells were incubated at 42°C for 2h, harvested and resuspended in 20 mM Tris HC1 (pH 7.4), 7mM 2 mercaptoethanol, ImM EDTA. The cells were lysed by freezing and thawing in the presence of lysozyme (lmg/ml) and ligthly sonicated. The low speed supernatant was precipitated by ammonium sulfate (60% saturated) and dissolved in the original buffer to be subjected to gel nitration through Sepharose 6B, followed by phosphocellulose colum (Pll) and DEAE cellulose colum (DE52). Purified gp8 appeared at 0.3M NaCl and formed crystals when its concentration increased above 1.5 mg/ml.

scholarly journals Three-dimensional structure of renal Na,K-ATPase determined by electron microscopy of membrane crystals

FEBS Letters ◽  
1985 ◽  
Vol 187 (1) ◽  
pp. 182-186 ◽  
Author(s):  
Hans Herbert ◽  
Elisabeth Skriver ◽  
Arvid B. Maunsbach
Keyword(s):  
Electron Microscopy ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Three Dimensional Structure
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