Chromosomal introgressions from Oryza meridionalis into domesticated rice Oryza sativa result in iron tolerance

Abstract Iron (Fe) toxicity is one of the most common mineral disorders affecting rice (Oryza sativa) production in flooded lowland fields. Oryza meridionalis is indigenous to northern Australia and grows in regions with Fe-rich soils, making it a candidate for use in adaptive breeding. With the aim of understanding tolerance mechanisms in rice, we screened a population of interspecific introgression lines from a cross between O. sativa and O. meridionalis for the identification of quantitative trait loci (QTLs) contributing to Fe-toxicity tolerance. Six putative QTLs were identified. A line carrying one introgression from O. meridionalis on chromosome 9 associated with one QTL was highly tolerant despite very high shoot Fe concentrations. Physiological, biochemical, ionomic, and transcriptomic analyses showed that the tolerance of the introgression lines could partly be explained by higher relative Fe retention in the leaf sheath and culm. We constructed the interspecific hybrid genome in silico for transcriptomic analysis and identified differentially regulated introgressed genes from O. meridionalis that could be involved in shoot-based Fe tolerance, such as metallothioneins, glutathione S-transferases, and transporters from the ABC and MFS families. This work demonstrates that introgressions of O. meridionalis into the O. sativa genome can confer increased tolerance to excess Fe.

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Introgression from Oryza meridionalis into domesticated rice Oryza sativa results in shoot-based iron tolerance

10.1101/2020.06.05.135947 ◽

2020 ◽

Author(s):

Andriele Wairich ◽

Ben Hur Neves de Oliveira ◽

Lin-Bo Wu ◽

Varunseelan Murugaiyan ◽

Marcia Margis-Pinheiro ◽

...

Keyword(s):

Oryza Sativa ◽

Leaf Sheath ◽

Chromosome 9 ◽

Physiological Basis ◽

Interspecific Introgression ◽

Oryza Meridionalis ◽

Hybrid Genome ◽

Glutathione S Transferases ◽

Fe Toxicity ◽

Iron Tolerance

AbstractIron (Fe) toxicity is one of the most common mineral disorders affecting rice (Oryza sativa) production in flooded lowland fields. Oryza meridionalis is endemic from Northern Australia and grows in regions with Fe rich soils, making it a candidate for use in adaptive breeding. Aiming to understand tolerance mechanisms in rice, we screened a population of interspecific introgression lines (IL) from a cross between O. sativa and O. meridionalis for the identification of QTLs contributing to Fe toxicity tolerance. Six putative QTLs were identified. A line carrying one introgression from O. meridionalis on chromosome 9 associated with one QTL was highly tolerant despite very high shoot Fe concentrations. Physiological, biochemical, ionomic and transcriptomic analyses showed that the IL tolerance could partly be explained by Fe retention in the leaf sheath and culm. We constructed the interspecific hybrid genome in silico for transcriptomic analysis, and identified differentially regulated introgressed genes from O. meridionalis that could be involved in shoot-based Fe tolerance, such as metallothioneins, glutathione S-transferases and transporters from ABC and MFS families. This is the first work to demonstrate that introgressions of O. meridionalis into the O. sativa genome can confer increased tolerance to excess Fe.HighlightWe identified QTLs associated with iron tolerance derived from O. meridionalis, and characterized their physiological basis in O. sativa.

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Genetic Dissection of Yield Potential in Rice (Oryza sativa L.) Using Introgression Lines

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2008.01500 ◽

2009 ◽

Vol 34 (9) ◽

pp. 1500-1509

Author(s):

Le KANG

Keyword(s):

Oryza Sativa ◽

Introgression Lines ◽

Yield Potential ◽

Genetic Dissection

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Yield QTL analysis of Oryza sativa x O. glumaepatula introgression lines

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2013000300006 ◽

2013 ◽

Vol 48 (3) ◽

pp. 280-286 ◽

Cited By ~ 2

Author(s):

Priscila Nascimento Rangel ◽

Rosana Pereira Vianello ◽

Arthur Tavares Oliveira Melo ◽

Paulo Hideo Nakano Rangel ◽

João Antônio Mendonça ◽

...

Keyword(s):

Oryza Sativa ◽

Interspecific Cross ◽

Rice Genome ◽

Introgression Lines ◽

Wild Accession ◽

Cultivated Rice ◽

Two Generations ◽

Ssr Loci ◽

Donor Parent ◽

Effect On Yield

The objective of this work was to evaluate the yield performance of two generations (BC2F2 and BC2F9) of introgression lines developed from the interspecific cross between Oryza sativa and O. glumaepatula, and to identify the SSR markers associated to yield. The wild accession RS‑16 (O. glumaepatula) was used as donor parent in the backcross with the high yielding cultivar Cica‑8 (O. sativa). A set of 114 BC2F1 introgression lines was genotyped with 141 polymorphic SSR loci distributed across the whole rice genome. Molecular analysis showed that in average 22% of the O. glumaepatula genome was introgressed into BC2F1 generation. Nine BC2F9 introgression lines had a significantly higher yield than the genitor Cica‑8, thus showing a positive genome interaction among cultivated rice and the wild O. glumaepatula. Seven QTL were identified in the overall BC2F2, with one marker interval (4879‑EST20) of great effect on yield. The alleles with positive effect on yield came from the cultivated parent Cica‑8.

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Using chromosome introgression lines to map quantitative trait loci for photosynthesis parameters in rice (Oryza sativa L.) leaves under drought and well-watered field conditions

Journal of Experimental Botany ◽

10.1093/jxb/err292 ◽

2011 ◽

Vol 63 (1) ◽

pp. 455-469 ◽

Cited By ~ 64

Author(s):

Junfei Gu ◽

Xinyou Yin ◽

Paul C. Struik ◽

Tjeerd Jan Stomph ◽

Huaqi Wang

Keyword(s):

Oryza Sativa ◽

Quantitative Trait Loci ◽

Quantitative Trait ◽

Oryza Sativa L ◽

Introgression Lines ◽

Field Conditions ◽

Trait Loci ◽

Chromosome Introgression

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Glutathione S-transferases modulate Cu tolerance in Oryza sativa

Environmental and Experimental Botany ◽

10.1016/j.envexpbot.2018.07.007 ◽

2018 ◽

Vol 155 ◽

pp. 313-320 ◽

Cited By ~ 38

Author(s):

Lu Li ◽

Mengjiao Hou ◽

Li Cao ◽

Yan Xia ◽

Zhenguo Shen ◽

...

Keyword(s):

Oryza Sativa ◽

Glutathione S Transferases ◽

Cu Tolerance

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Sarocladium oryzae. [Descriptions of Fungi and Bacteria].

IMI Descriptions of Fungi and Bacteria ◽

10.1079/dfb/20056400673 ◽

1980 ◽

Author(s):

B. L. K. Brady

Keyword(s):

Oryza Sativa ◽

Geographical Distribution ◽

Leaf Sheath ◽

Fungal Attack ◽

Rice Disease ◽

Sheath Rot

Abstract A description is provided for Sarocladium oryzae. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOST: Oryza sativa (rice). DISEASE: Sheath rot. This occurs in the upper leaf sheath enclosing young panicles and starts as oblong or irregular lesions with brownish margins and greyish centres, 0.5-1.5 cm long, which later enlarge and coalesce. Whitish mycelium may be seen in the centre of the lesions and occasionally inside the rotted sheaths. Severely affected panicles do not emerge, the effect being known as choking. The symptoms are evidently indistinguishable from those associated with S. attenuatum. GEOGRAPHICAL DISTRIBUTION: India, Bangladesh, Brunei, Kenya, Nigeria. Although it is not always possible when 'Acrocydndrium oryzae' is reported in the earlier literature to know whether S. oryzae or S. attenuatum is intended, the distribution of the former extends into S.E. Asia and the latter into N. America. TRANSMISSION: By conidial dispersal. Fungal attack is often associated with damage by pests - the boring beetle, Corticarina gibbosa (Amin et al.) or the mite Steneotarsonemus madecassus (Hsieh et al., 1977 with 'Acrocylindrium oryzae') and S. spinki (Chien & Huang, 1979).

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Transgressive Variation for Yield Components Measured throughout the Growth Cycle of Jefferson Rice (Oryza sativa ) × O. rufipogon Introgression Lines

Crop Science ◽

10.2135/cropsci2015.10.0603 ◽

2016 ◽

Vol 56 (5) ◽

pp. 2336-2347 ◽

Cited By ~ 2

Author(s):

Georgia C. Eizenga ◽

Jeremy D. Edwards ◽

Kathleen M. Yeater ◽

Susan R. McCouch ◽

Anna M. McClung

Keyword(s):

Oryza Sativa ◽

Yield Components ◽

Growth Cycle ◽

Introgression Lines

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Reinventing heterosis phenomenon through deployment of alien introgression lines in rice ( Oryza sativa L.)

Plant Breeding ◽

10.1111/pbr.12692 ◽

2019 ◽

Vol 138 (3) ◽

pp. 277-289

Author(s):

Kiran B. Gaikwad ◽

Naveen Singh ◽

Dharminder Bhatia ◽

Rupinder Kaur ◽

Navtej S. Bains ◽

...

Keyword(s):

Oryza Sativa ◽

Oryza Sativa L ◽

Introgression Lines ◽

Alien Introgression

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Water extraction and root traits in Oryza sativa × Oryza glaberrima introgression lines under different soil moisture regimes

Functional Plant Biology ◽

10.1071/fp12163 ◽

2013 ◽

Vol 40 (1) ◽

pp. 54 ◽

Cited By ~ 13

Author(s):

Abubakary A. Kijoji ◽

Susan Nchimbi-Msolla ◽

Zakaria L. Kanyeka ◽

Stephen P. Klassen ◽

Rachid Serraj ◽

...

Keyword(s):

Oryza Sativa ◽

Drought Stress ◽

Water Uptake ◽

Root Traits ◽

Genetic Differences ◽

Introgression Lines ◽

Sub Saharan Africa ◽

Future Research ◽

Oryza Glaberrima ◽

Soil Drying

Drought is a major constraint to rainfed rice production in some parts of sub Saharan Africa (SSA) because of irregular and low rainfall. Improving root water uptake during progressive soil drying could contribute to terminal drought stress resistance. Two lysimeter experiments were conducted using 200 introgression lines (ILs) derived from Oryza sativa L. × Oryza glaberrima Steud. crosses (IR64/RAM54 and IR64/RAM90) to characterise water uptake and root traits under progressive soil drying and to assess genetic differences among these lines in relation to drought stress. The lysimeter facility setup and protocols for simultaneous monitoring of water uptake rates and leaf area are described. Significant genetic variability for water uptake and root traits among the ILs was observed under drought stress. Water uptake in the drought stress treatments was correlated with root length density (RLD) and root dry matter (RDM) at lower soil depths. The highest RLD values at depth were observed among the RAM90 ILs and genetic differences were observed between RAM90 and RAM54 populations, implying that accession RAM90 could be used as a source for improving drought avoidance in lowland rice. The ILs with greater water uptake identified in this study will be used in future research for improving drought resistance of rice in SSA.

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