scholarly journals Comparison of cauliflower mosaic virus 35S and nopaline synthase promoters in transgenic plants

1987 ◽  
Vol 15 (4) ◽  
pp. 1543-1558 ◽  
Author(s):  
P.R. Sanders ◽  
J.A. Winter ◽  
A.R. Barnason ◽  
S.G. Rogers ◽  
R.T. Fraley
Keyword(s):  
Transgenic Plants ◽  
Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
Cauliflower Mosaic Virus 35S

Multiple cis Regulatory Elements for Maximal Expression of the Cauliflower Mosaic Virus 35S Promoter in Transgenic Plants

1989 ◽  
Vol 1 (1) ◽  
pp. 141 ◽  
Author(s):  
Rong-Xiang Fang ◽  
Ferenc Nagy ◽  
Shanthi Sivasubramaniam ◽  
Nam-Hai Chua
Keyword(s):  
Transgenic Plants ◽  
Mosaic Virus ◽  
Regulatory Elements ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Cauliflower Mosaic Virus 35S ◽  
Maximal Expression

Differential inhibition of downstream gene expression by the cauliflower mosaic virus 35S RNA leader

Virus Genes ◽  
1989 ◽  
Vol 3 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Johannes F�tterer ◽  
Karl Gordon ◽  
Pierre Pfeiffer ◽  
H�l�lene Sanfa�on ◽  
Barbara Pisan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
Differential Inhibition ◽  
Downstream Gene Expression ◽  
Cauliflower Mosaic Virus 35S

scholarly journals Altered Patterns of Gene Expression in Arabidopsis Elicited by Cauliflower Mosaic Virus (CaMV) Infection and by a CaMV Gene VI Transgene

1999 ◽  
Vol 12 (5) ◽  
pp. 377-384 ◽  
Author(s):  
Chiara Geri ◽  
Edi Cecchini ◽  
Maria E. Giannakou ◽  
Simon N. Covey ◽  
Joel J. Milner
Keyword(s):  
Gene Expression ◽  
Transgenic Plants ◽  
Mosaic Virus ◽  
Rna Binding ◽  
Important Determinant ◽  
Blot Hybridization ◽  
Cauliflower Mosaic Virus ◽  
Slot Blot Hybridization ◽  
Pcr Products ◽  
Polymerase Chain

Cauliflower mosaic virus (CaMV) gene VI protein (P6) is an important determinant of symptom expression. Differential display polymerase chain reaction (PCR) was used to identify changes in gene expression in Arabidopsis elicited by a P6 transgene that causes a symptomatic phenotype. We used slot blot hybridization to measure the abundance of mRNAs complementary to 66 candidate PCR products in transgenic, CaMV-infected, and uninfected Arabidopsis plants. CaMV-infected and P6 transgenic plants showed broadly similar changes in abundance of mRNA species. In P6 transgenic plants we detected 18 PCR products that showed unambiguous changes in abundance plus another 15 that showed more limited changes (approximately twofold). CaMV-infected plants showed 17 unambiguous and 13 limited changes. Down-regulated species include those encoding a novel, phenol-like sulfotransferase, and a glycine-rich, RNA-binding protein. Up-regulated species included ones encoding an myb protein, glycine-rich and stress-inducible proteins, and a member of a previously unreported gene family. CaMV infection causes alterations in expression of many Arabidopsis genes. Transgene-mediated expression of P6 mimics virus infection in its effect on host gene expression, providing a potential mechanism for this process.

Functional analysis of cauliflower mosaic virus 35S promoter: re-evaluation of the role of subdomains B5, B4 and B2 in promoter activity

2007 ◽  
Vol 5 (6) ◽  
pp. 696-708 ◽  
Author(s):  
Simran Bhullar ◽  
Sudipta Datta ◽  
Sonia Advani ◽  
Suma Chakravarthy ◽  
Taru Gautam ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Mosaic Virus ◽  
Promoter Activity ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Cauliflower Mosaic Virus 35S

scholarly journals Production of Human Papillomavirus Type 16 Virus-Like Particles in Transgenic Plants

2003 ◽  
Vol 77 (17) ◽  
pp. 9211-9220 ◽  
Author(s):  
Sophia Biemelt ◽  
Uwe Sonnewald ◽  
Petra Galmbacher ◽  
Lothar Willmitzer ◽  
Martin Müller
Keyword(s):  
Transgenic Plants ◽  
Mosaic Virus ◽  
Transgenic Tobacco ◽  
Protein Modification ◽  
Human Papillomaviruses ◽  
Cauliflower Mosaic Virus ◽  
Total Soluble Protein ◽  
Virus Like Particles ◽  
Potato Plants ◽  
L1 Protein

ABSTRACT Cervical cancer is linked to infection with human papillomaviruses (HPV) and is the third most common cancer among women worldwide. There is a strong demand for the development of an HPV preventive vaccine. Transgenic plants expressing the HPV major capsid protein L1 could be a system to produce virus-like particles for prophylactic vaccination or could even be used as edible vaccines to induce an L1-specific prophylactic immune response. Here, we describe the generation of transgenic tobacco and potato plants carrying the HPV type 16 major structural gene L1 under the control of the cauliflower mosaic virus 35S promoter. All attempts to express either the original, unmodified L1 gene or an L1 gene with a codon usage optimized for expression in plants failed. Surprisingly, small amounts of the protein were detected using an L1 gene optimized for expression in human cells. However, Northern blot analysis revealed that most of the L1 transcripts were degraded. Introduction of the translational enhancer Ω derived from the tobacco mosaic virus strongly increased transcript stability and resulted in accumulation of L1 protein to approximately 0.5 to 0.2% of total soluble protein in transgenic tobacco and potato plants, respectively. The plant-derived L1 protein displayed conformation-specific epitopes and assembled into virus-like particles. Furthermore, we did not find any indications of protein modification of the L1 protein produced in plants. Plant-derived L1 was as immunogenic as L1 expressed in baculovirus-infected insect cells. Feeding of tubers from transgenic potatoes to mice induced an anti-L1 antibody response in 3 out of 24 mice, although this response was only transient in two of the mice. Our data, however, indicate that an anti-L1 response was primed in about half of the 24 animals.

scholarly journals The Cauliflower Mosaic Virus 35S Promoter Extends into the Transcribed Region

2004 ◽  
Vol 78 (22) ◽  
pp. 12120-12128 ◽  
Author(s):  
Sandra Pauli ◽  
Helen M. Rothnie ◽  
Gang Chen ◽  
Xiaoyuan He ◽  
Thomas Hohn
Keyword(s):  
Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Sequence Motifs ◽  
Transcription Start ◽  
Transcriptional Enhancers ◽  
Enhance Gene Expression ◽  
Enhancer Function ◽  
Nucleotide Region ◽  
Cauliflower Mosaic Virus 35S

ABSTRACT A 60-nucleotide region (S1) downstream of the transcription start site of the cauliflower mosaic virus 35S RNA can enhance gene expression. By using transient expression assays with plant protoplasts, this activity was shown to be at least partially due to the effect of transcriptional enhancers within this region. We identify sequence motifs with enhancer function, which are normally masked by the powerful upstream enhancers of the 35S promoter. A repeated CT-rich motif is involved both in enhancer function and in interaction with plant nuclear proteins. The S1 region can also enhance expression from heterologous promoters.

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