scholarly journals Complementation of the xeroderma pigmentosum DNA repair synthesis defect withEscherichia coliUvrABC proteins in a cell-free system

1990 ◽  
Vol 18 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Johan Hansson ◽  
Lawrence Grossman ◽  
Tomas Lindahl ◽  
Richard D. Wood
Keyword(s):  
Dna Repair ◽  
Xeroderma Pigmentosum ◽  
Repair Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell ◽  
Dna Repair Synthesis

UV-induced DNA repair synthesis in cells of patients with different forms of xeroderma pigmentosum and of heterozygotes

1973 ◽  
Vol 20 (3) ◽  
pp. 417-428 ◽  
Author(s):  
W.J. Kleijer ◽  
E.A. De Weerd-Kastelein ◽  
M.L. Sluyter ◽  
W. Keijzer ◽  
J. De Wit ◽  
...  
Keyword(s):  
Dna Repair ◽  
Xeroderma Pigmentosum ◽  
Repair Synthesis ◽  
Dna Repair Synthesis ◽  
In Cells

Xeroderma pigmentosum D-HeLa hybrids with low and high ultraviolet sensitivity associated with normal and diminished DNA repair ability, respectively

1985 ◽  
Vol 76 (1) ◽  
pp. 115-133
Author(s):  
R.T. Johnson ◽  
S. Squires ◽  
G.C. Elliott ◽  
G.L. Koch ◽  
A.J. Rainbow
Keyword(s):  
Dna Repair ◽  
Xeroderma Pigmentosum ◽  
Excision Repair ◽  
Complementation Group ◽  
Repair Capacity ◽  
Repair Synthesis ◽  
Repair Ability ◽  
Dna Repair Synthesis ◽  
Host Cell Reactivation ◽  
Group D

Fusion between HeLa and fibroblasts from complementation group D xeroderma pigmentosum (XPD) followed by challenge with small doses of ultraviolet light (u.v.) results in the production of hybrid cells expressing either HeLa (HD1) or XPD-like (HD2) sensitivity to u.v. and related repair capacity. Assays used included unscheduled DNA synthesis (UDS), DNA break accumulation in the presence of inhibitors of DNA repair synthesis and host cell reactivation of irradiated adenovirus. Complementation assay in heterokaryons reveals limited ability of HD2 to restore UDS in XPD nuclei. We believe this complementation is more apparent than real since proliferating hybrids of HD2 and XPD parentage are without exception u.v.-sensitive and express limited excision repair. On the other hand hybrids between HD2 and XPC, XPE or XPF fibroblasts show true complementation resulting in a return to normal u.v. sensitivity and elevated repair ability.

scholarly journals Effect of detergents on sterol synthesis in a cell-free system of yeast

1982 ◽  
Vol 23 (6) ◽  
pp. 803-810
Author(s):  
S Hata ◽  
T Nishino ◽  
N Ariga ◽  
H Katsuki
Keyword(s):  
Sterol Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals Localization of DNA repair synthesis by human cell extracts to a short region at the site of a lesion

1989 ◽  
Vol 264 (36) ◽  
pp. 21788-21792
Author(s):  
J Hansson ◽  
M Munn ◽  
W D Rupp ◽  
R Kahn ◽  
R D Wood
Keyword(s):  
Dna Repair ◽  
Human Cell ◽  
Repair Synthesis ◽  
Short Region ◽  
Cell Extracts ◽  
Dna Repair Synthesis

scholarly journals Reconstitution of Endosomal Proteolysis in a Cell-free System

1989 ◽  
Vol 264 (10) ◽  
pp. 5392-5399
Author(s):  
L S Mayorga ◽  
R Diaz ◽  
P D Stahl
Keyword(s):  
Free System ◽  
Cell Free System ◽  
A Cell
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