Various Levels of DNA Repair Synthesis in Xeroderma Pigmentosum Cells exposed to the Carcinogens N-Hydroxy and N-Acetoxy-2-acetyl-aminofluorene

1972 ◽  
Vol 238 (79) ◽  
pp. 9-10 ◽  
Author(s):  
H. F. STICH ◽  
R. H. C. SAN ◽  
J. A. MILLER ◽  
E. C. MILLER
Keyword(s):  
Dna Repair ◽  
Xeroderma Pigmentosum ◽  
Repair Synthesis ◽  
Dna Repair Synthesis

UV-induced DNA repair synthesis in cells of patients with different forms of xeroderma pigmentosum and of heterozygotes

1973 ◽  
Vol 20 (3) ◽  
pp. 417-428 ◽  
Author(s):  
W.J. Kleijer ◽  
E.A. De Weerd-Kastelein ◽  
M.L. Sluyter ◽  
W. Keijzer ◽  
J. De Wit ◽  
...  
Keyword(s):  
Dna Repair ◽  
Xeroderma Pigmentosum ◽  
Repair Synthesis ◽  
Dna Repair Synthesis ◽  
In Cells

Xeroderma pigmentosum D-HeLa hybrids with low and high ultraviolet sensitivity associated with normal and diminished DNA repair ability, respectively

1985 ◽  
Vol 76 (1) ◽  
pp. 115-133
Author(s):  
R.T. Johnson ◽  
S. Squires ◽  
G.C. Elliott ◽  
G.L. Koch ◽  
A.J. Rainbow
Keyword(s):  
Dna Repair ◽  
Xeroderma Pigmentosum ◽  
Excision Repair ◽  
Complementation Group ◽  
Repair Capacity ◽  
Repair Synthesis ◽  
Repair Ability ◽  
Dna Repair Synthesis ◽  
Host Cell Reactivation ◽  
Group D

Fusion between HeLa and fibroblasts from complementation group D xeroderma pigmentosum (XPD) followed by challenge with small doses of ultraviolet light (u.v.) results in the production of hybrid cells expressing either HeLa (HD1) or XPD-like (HD2) sensitivity to u.v. and related repair capacity. Assays used included unscheduled DNA synthesis (UDS), DNA break accumulation in the presence of inhibitors of DNA repair synthesis and host cell reactivation of irradiated adenovirus. Complementation assay in heterokaryons reveals limited ability of HD2 to restore UDS in XPD nuclei. We believe this complementation is more apparent than real since proliferating hybrids of HD2 and XPD parentage are without exception u.v.-sensitive and express limited excision repair. On the other hand hybrids between HD2 and XPC, XPE or XPF fibroblasts show true complementation resulting in a return to normal u.v. sensitivity and elevated repair ability.

scholarly journals Complementation of the xeroderma pigmentosum DNA repair synthesis defect withEscherichia coliUvrABC proteins in a cell-free system

1990 ◽  
Vol 18 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Johan Hansson ◽  
Lawrence Grossman ◽  
Tomas Lindahl ◽  
Richard D. Wood
Keyword(s):  
Dna Repair ◽  
Xeroderma Pigmentosum ◽  
Repair Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell ◽  
Dna Repair Synthesis

scholarly journals Localization of DNA repair synthesis by human cell extracts to a short region at the site of a lesion

1989 ◽  
Vol 264 (36) ◽  
pp. 21788-21792
Author(s):  
J Hansson ◽  
M Munn ◽  
W D Rupp ◽  
R Kahn ◽  
R D Wood
Keyword(s):  
Dna Repair ◽  
Human Cell ◽  
Repair Synthesis ◽  
Short Region ◽  
Cell Extracts ◽  
Dna Repair Synthesis

scholarly journals Enhanced DNA Repair Synthesis in Hyperacetylated Nucleosomes

1989 ◽  
Vol 264 (19) ◽  
pp. 11026-11034
Author(s):  
B Ramanathan ◽  
M J Smerdon
Keyword(s):  
Dna Repair ◽  
Repair Synthesis ◽  
Dna Repair Synthesis

Induction of DNA repair synthesis by ultraviolet radiation and methylmethanesulphonate in cultured mouse lymphocytes

1983 ◽  
Vol 18 (1) ◽  
pp. 21-27 ◽  
Author(s):  
V. Bianchi ◽  
A. Zantedeschi ◽  
F. Ronchese ◽  
A.G. Levis
Keyword(s):  
Dna Repair ◽  
Ultraviolet Radiation ◽  
Repair Synthesis ◽  
Dna Repair Synthesis ◽  
Mouse Lymphocytes

DNA repair synthesis-related enzymes during spermatogenesis in the mouse

1984 ◽  
Vol 153 (2) ◽  
pp. 499-505 ◽  
Author(s):  
P. Orlando ◽  
P. Grippo ◽  
R. Geremia
Keyword(s):  
Dna Repair ◽  
Repair Synthesis ◽  
Dna Repair Synthesis

scholarly journals DNA repair synthesis in human heterokaryons. III. The rapid and slow complementing varieties of xeroderma pigmentosum

1976 ◽  
Vol 20 (1) ◽  
pp. 207-213
Author(s):  
F. Giannelli ◽  
S.A. Pawsey
Keyword(s):  
Protein Synthesis ◽  
Xeroderma Pigmentosum ◽  
Excision Repair ◽  
Dependent Manner ◽  
Repair Synthesis ◽  
Normal Cells ◽  
Complementation Groups ◽  
Dna Repair Synthesis ◽  
And Control ◽  
The Relationship

Patients with Xeroderma pigmentosum and defective DNA excision repair can be distinguished as a rapid (r-XP) and slow (s-XP) complementing variety. When fused with normal cells, fibroblasts from the r-XP are complemented rapidly and in the absence of protein synthesis while those from the s-XP are complemented slowly by a process partly, but not entirely, dependent on protein synthesis. Heterokaryons with different ratios of r-XP to s-XP nuclei (i.e. 1:1-5 and 1-5:1) and control heterokaryons containing one normal and 1-5 r- or s-XP nuclei show that if cell fusion and incubation is conducted in medium preventing protein synthesis, the rXP cells do not complement the s-XP partner at all and, conversely, that the latter is not as effective as normal cells at complementing the rXP partner. On the contrary, if protein synthesis is permitted, the 2 types of XP cells complement each other in a gene dose-dependent manner and to an extent similar to that observed in the control heterokaryons. These findings indicate that the r- and s-XP varieties are caused by mutations at different loci and suggest that the products of these loci interact to produce a functional unit which is present in normal control cells but absent in the XP strains. The relationship between the complementation groups described here and those already reported in the literature being investigated.

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