Tsp49I (ACGT/), a thermostable neoschizomer of the Type II restriction endonuclease MaeII (A/CGT), discovered in isolates of the genus Thermus from the Azores, Iceland and New Zealand

The Type I simple cold block device was described by Bullivant and Ames in 1966 and represented the product of the first successful effort to simplify the equipment required to do sophisticated freeze-cleave techniques. Bullivant, Weinstein and Someda described the Type II device which is a modification of the Type I device and was developed as a collaborative effort at the Massachusetts General Hospital and the University of Auckland, New Zealand. The modifications reduced specimen contamination and provided controlled specimen warming for heat-etching of fracture faces. We have now tested the Mass. General Hospital version of the Type II device (called the “Type II-MGH device”) on a wide variety of biological specimens and have established temperature and pressure curves for routine heat-etching with the device.

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NEW ZEALAND TYPE II ABORTION IN EWES.

Australian Veterinary Journal ◽

10.1111/j.1751-0813.1954.tb08204.x ◽

1954 ◽

Vol 30 (7) ◽

pp. 216-218 ◽

Cited By ~ 48

Author(s):

W. J. Hartley ◽

J. L. Jebson ◽

D. McFarlane

Keyword(s):

New Zealand ◽

Type Ii

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4693978 Type II restriction endonuclease maei, a process for obtaining it and the use thereof

Biotechnology Advances ◽

10.1016/0734-9750(88)90599-x ◽

1988 ◽

Vol 6 (1) ◽

pp. 76

Keyword(s):

Restriction Endonuclease ◽

Type Ii

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Continuing Mycobacterium bovis transmission from animals to humans in New Zealand

Epidemiology and Infection ◽

10.1017/s0950268806005930 ◽

2006 ◽

Vol 134 (5) ◽

pp. 1068-1073 ◽

Cited By ~ 33

Author(s):

M. G. BAKER ◽

L. D. LOPEZ ◽

M. C. CANNON ◽

G. W. DE LISLE ◽

D. M. COLLINS

Keyword(s):

New Zealand ◽

Restriction Endonuclease ◽

Mycobacterium Bovis ◽

Restriction Endonuclease Analysis ◽

Domestic Animals ◽

Human Infection ◽

Potential Contribution ◽

Large Reservoir ◽

Animal Reservoirs ◽

Endonuclease Analysis

New Zealand has a large reservoir of Mycobacterium bovis infection in wild and farmed animals. This study aimed to assess the extent of human infection with this organism and the potential contribution of these animal sources. Combined epidemiological and laboratory investigation of human tuberculosis cases over the period 1995–2002 showed that M. bovis accounted for 2·7% (54/1997) of laboratory-confirmed human tuberculosis cases, a rate of 0·2/100000 population. M. bovis isolates from humans (23) were typed using restriction endonuclease analysis (REA) and compared with isolates from wild and domestic animals (2600). Fourteen (61%) of the human isolates had REA patterns that were identical to patterns for isolates from cattle, deer, possums, ferrets, pigs, and occasionally cats. These results suggest a low level of ongoing M. bovis transmission from animal reservoirs to humans in New Zealand.

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