scholarly journals UP element-dependent transcription at the Escherichia coli rrnB P1 promoter: positional requirements and role of the RNA polymerase alpha subunit linker

2001 ◽  
Vol 29 (20) ◽  
pp. 4166-4178 ◽  
Author(s):  
W. Meng
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Alpha Subunit

Converting Escherichia coli RNA Polymerase into an Enhancer-Responsive Enzyme: Role of an NH2-Terminal Leucine Patch in sigma54

Science ◽  
1995 ◽  
Vol 270 (5238) ◽  
pp. 992-994 ◽  
Author(s):  
J. T. Wang ◽  
A. Syed ◽  
M. Hsieh ◽  
J. D. Gralla
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase

scholarly journals Intrasubunit nucleotide binding in ribonucleic acid polymerase

1978 ◽  
Vol 175 (1) ◽  
pp. 189-192 ◽  
Author(s):  
A D B Malcolm ◽  
J R Moffatt
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Ribonucleic Acid ◽  
Periodate Oxidation ◽  
Nucleotide Binding ◽  
Alpha Subunit ◽  
Nucleoside Triphosphate ◽  
Competitive Inhibitors ◽  
Nucleoside Triphosphates ◽  
Derivatives Of

1. Periodate oxidation of the ribose ring was used to synthesize derivatives of nucleoside triphosphates. 2. These oxidized nucleoside triphosphates. 2. These oxidized nucleoside triphosphates are competitive inhibitors of RNA polymerase. 3. On incubation, together with NaBH4, these oxidized labelled nucleotides are covalently bound to Escherichia coli RNA polymerase. 4. Nucleoside triphosphate substrates decrease the extent of labelling. 5. A lysine residue in an alpha-subunit is labelled. 6. The significance of these results in relation to the location of the nucleotide-binding site is discussed.

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