scholarly journals Influence of major-groove chemical modifications of DNA on transcription by bacterial RNA polymerases

2016 ◽  
Vol 44 (7) ◽  
pp. 3000-3012 ◽  
Author(s):  
Veronika Raindlová ◽  
Martina Janoušková ◽  
Michaela Slavíčková ◽  
Pavla Perlíková ◽  
Soňa Boháčová ◽  
...  
Author(s):  
John Harbottle ◽  
Hamed Mosaei ◽  
Nicholas Allenby ◽  
Nikolay Zenkin

Rifamycins, such as rifampicin, are potent inhibitors of bacterial RNA polymerases and widely used antibiotics. Usually rifamycin-resistance is associated with mutations in RNAP that preclude rifamycins binding. However, some bacteria have ADP-ribosyl transferases Arr that ADP-ribosylate rifamycin molecules, thus inactivating their antimicrobial activity. Here we directly show that ADP-ribosylation abolishes inhibition of transcription by rifampicin, the most widely used rifamycin antibiotic. We also show that natural rifamycin, Kanglemycin A, which has a unique sugar moiety at the ansa-chain close to the Arr-modification site, does not bind to Arr from M. smegmatis , and thus is not susceptible to inactivation. We, however, found that Kanglemycin A can still be ADP-ribosylated by Arr of an emerging pathogen M. abscessus . Interestingly, the only part of Arr which exhibits no homology between the species is the part that sterically clashes with sugar moiety of Kanglemycin A in M. smegmatis Arr. This suggests that M. abscessus has encountered KglA or rifamycin with similar sugar modification in the course of evolution. The results show that KglA could be effective antimicrobial against some of the Arr encoding bacteria.


2016 ◽  
Vol 44 (3) ◽  
pp. 1298-1308 ◽  
Author(s):  
Daria Esyunina ◽  
Matti Turtola ◽  
Danil Pupov ◽  
Irina Bass ◽  
Saulius Klimašauskas ◽  
...  

1979 ◽  
Vol 13 (1) ◽  
pp. 59-97 ◽  
Author(s):  
T Yura ◽  
A Ishihama

2017 ◽  
Vol 292 (16) ◽  
pp. 6744-6752 ◽  
Author(s):  
Nataliya Miropolskaya ◽  
Daria Esyunina ◽  
Andrey Kulbachinskiy

2019 ◽  
Vol 10 (14) ◽  
pp. 3937-3942 ◽  
Author(s):  
Zuzana Vaníková ◽  
Martina Janoušková ◽  
Milada Kambová ◽  
Libor Krásný ◽  
Michal Hocek

Biomimetic switching of in vitro transcription was developed by photochemical deprotection of photocaged 5hmU or 5hmC in template DNA (ON) and by enzymatic phosphorylation (OFF).


1997 ◽  
Vol 60 (8) ◽  
pp. 858-861 ◽  
Author(s):  
Shichang Miao ◽  
Marcia R. Anstee ◽  
Kelly LaMarco ◽  
Joseph Matthew ◽  
Linda H. T. Huang ◽  
...  

2009 ◽  
Vol 284 (20) ◽  
pp. 13497-13504 ◽  
Author(s):  
Tatyana Kent ◽  
Ekaterina Kashkina ◽  
Michael Anikin ◽  
Dmitry Temiakov

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