scholarly journals Specificity of promoter site utilization in vitro by bacterial RNA polymerases on Bacillus phage phi 29 DNA. Transcription mapping with exonuclease III.

1980 ◽  
Vol 255 (18) ◽  
pp. 8819-8830 ◽  
Author(s):  
B.L. Davison ◽  
C.L. Murray ◽  
J.C. Rabinowitz
Keyword(s):  
Rna Polymerases ◽  
Exonuclease Iii ◽  
Dna Transcription ◽  
Bacterial Rna ◽  
Bacillus Phage

scholarly journals Transcription of 4′-thioDNA templates to natural RNA in vitro and in mammalian cells

2015 ◽  
Vol 51 (37) ◽  
pp. 7887-7890 ◽  
Author(s):  
Hideto Maruyama ◽  
Kazuhiro Furukawa ◽  
Hiroyuki Kamiya ◽  
Noriaki Minakawa ◽  
Akira Matsuda
Keyword(s):  
Nucleic Acids ◽  
Mammalian Cells ◽  
Genetic Material ◽  
Rna Polymerases ◽  
Chemically Modified ◽  
Biological Studies ◽  
Modified Nucleic Acids

Synthetic chemically modified nucleic acids, which are compatible with DNA/RNA polymerases, have great potential as a genetic material for synthetic biological studies.

scholarly journals Development of an on-disc isothermal in vitro amplification and detection of bacterial RNA

2017 ◽  
Vol 239 ◽  
pp. 235-242 ◽  
Author(s):  
Des Brennan ◽  
Helena Coughlan ◽  
Eoin Clancy ◽  
Nikolay Dimov ◽  
Thomas Barry ◽  
...  
Keyword(s):  
Bacterial Rna

RNAP subunits F/E (RPB4/7) are stably associated with archaeal RNA polymerase: using fluorescence anisotropy to monitor RNAP assembly in vitro

2009 ◽  
Vol 421 (3) ◽  
pp. 339-343 ◽  
Author(s):  
Dina Grohmann ◽  
Angela Hirtreiter ◽  
Finn Werner
Keyword(s):  
Rna Polymerase ◽  
Molecular Interactions ◽  
Fluorescence Anisotropy ◽  
Rna Polymerases ◽  
Dynamic Exchange ◽  
Transcription Cycle

Archaeal and eukaryotic RNAPs (DNA-dependent RNA polymerases) are complex multi-subunit enzymes. Two of the subunits, F and E, which together form the F/E complex, have been hypothesized to associate with RNAP in a reversible manner during the transcription cycle. We have characterized the molecular interactions between the F/E complex and the RNAP core. F/E binds to RNAP with submicromolar affinity and is not in a dynamic exchange with unbound F/E.

Effect of ionic strength on adenovirus 2 DNA transcription by KB cell DNA-dependent RNA polymerases I, II, and III

1974 ◽  
Vol 45 (3) ◽  
pp. 294-297 ◽  
Author(s):  
A. Sergeant ◽  
J. C. D'Halluin ◽  
A. P. Verbert ◽  
V. Krsmanovic
Keyword(s):  
Ionic Strength ◽  
Rna Polymerases ◽  
Dna Transcription

A thermostable protein factor acting on in vitro DNA transcription

1971 ◽  
Vol 45 (6) ◽  
pp. 1597-1607 ◽  
Author(s):  
M. Jacquet ◽  
R. Cukier-Kahn ◽  
J. Pla ◽  
F. Gros
Keyword(s):  
Dna Transcription ◽  
Protein Factor ◽  
Thermostable Protein

Fluorescent primer-based in vitro transcription system of viral RNA-dependent RNA polymerases

2013 ◽  
Vol 433 (2) ◽  
pp. 92-94
Author(s):  
Qiang Wang ◽  
Leiyun Weng ◽  
Hongbing Jiang ◽  
Shijian Zhang ◽  
Tetsuya Toyoda
Keyword(s):  
In Vitro Transcription ◽  
Rna Polymerases ◽  
Viral Rna ◽  
Transcription System

Estrogen-inducible binding of a nuclear factor to the vitellogenin upstream region

1988 ◽  
Vol 8 (10) ◽  
pp. 4557-4560
Author(s):  
O Bakker ◽  
J N Philipsen ◽  
B C Hennis ◽  
G Ab
Keyword(s):  
Dimethyl Sulfate ◽  
Upstream Region ◽  
Nuclear Factor ◽  
Base Pairs ◽  
Exonuclease Iii ◽  
Factor I ◽  
Nuclear Factor I ◽  
Vitellogenin Gene

The estrogen-dependent binding of a protein to the upstream region of the chicken vitellogenin gene was detected by using in vivo dimethyl sulfate, genomic DNase I, and in vitro exonuclease III footprinting. The site is located between base pairs -848 and -824, and its sequence resembles that of the nuclear factor I binding site. The results suggest that a nuclear factor binding to this site is involved in the regulation of the vitellogenin gene.

Adenovirus DNA: Transcription During Productive Infection, Integration in Transformed Cells, and Replication in Vitro

1975 ◽  
pp. 217-236
Author(s):  
Maurice Green ◽  
Tadashi Yamashita ◽  
Werner Büttner ◽  
Kei Fujinaga ◽  
Max Arens ◽  
...  
Keyword(s):  
Productive Infection ◽  
Transformed Cells ◽  
Dna Transcription
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