Pentoxifylline potentiates ionophore (A23187) mediated acrosome reaction in human sperm: flow cytometric analysis using CD46 antibody

1994 ◽  
Vol 9 (1) ◽  
pp. 71-76 ◽  
Author(s):  
J.A. Carver-Ward ◽  
K.A. Jaroudi ◽  
M. Einspenner ◽  
R.S. Parhar ◽  
S.T. Al-Sedairy ◽  
...  
Keyword(s):  
Acrosome Reaction ◽  
Flow Cytometric Analysis ◽  
Human Sperm ◽  
Ionophore A23187 ◽  
Flow Cytometric

scholarly journals Progesterone does not potentiate the acrosome reaction in human spermatozoa: flow cytometric analysis using CD46 antibody

1996 ◽  
Vol 11 (1) ◽  
pp. 121-126 ◽  
Author(s):  
J.A. Carver-Ward ◽  
J.M.G. Hollanders ◽  
K.A. Jaroudi ◽  
M. Einspenner ◽  
S.T. Al-Sedairy ◽  
...  
Keyword(s):  
Acrosome Reaction ◽  
Flow Cytometric Analysis ◽  
Human Spermatozoa ◽  
Flow Cytometric

Centrifugation on Percoll Gradient Enhances Fluorescent Lectin Binding on Human Sperm: A Flow Cytometric Analysis

1996 ◽  
Vol 37 (3) ◽  
pp. 179-187 ◽  
Author(s):  
E. De Maistre ◽  
M. C. Béné ◽  
B. Foliguet ◽  
F. Touati ◽  
G. C. Faure
Keyword(s):  
Lectin Binding ◽  
Flow Cytometric Analysis ◽  
Human Sperm ◽  
Percoll Gradient ◽  
Flow Cytometric

Evidence for the activation of phospholipases during acrosome reaction of human sperm elicited by calcium ionophore A23187

1987 ◽  
Vol 919 (3) ◽  
pp. 255-265 ◽  
Author(s):  
Pierre J. Bennet ◽  
Jean-Pierre Moatti ◽  
Ariette Mansat ◽  
Huguette Ribbes ◽  
Jean-Claude Cayrac ◽  
...  
Keyword(s):  
Acrosome Reaction ◽  
Calcium Ionophore ◽  
Human Sperm ◽  
Calcium Ionophore A23187 ◽  
Ionophore A23187

scholarly journals Automation of human sperm cell analysis by flow cytometry

1997 ◽  
Vol 43 (5) ◽  
pp. 801-807 ◽  
Author(s):  
Fulvio Ferrara ◽  
Rita Daverio ◽  
Giuliano Mazzini ◽  
Pierangelo Bonini ◽  
Giuseppe Banfi
Keyword(s):  
Flow Cytometry ◽  
Flow Cytometric Analysis ◽  
Human Sperm ◽  
Sperm Analysis ◽  
Cell Counts ◽  
Flow Cytometric ◽  
Sperm Counts ◽  
The Mean ◽  
Nucleic Acid Stain ◽  
Automated Methods

Abstract Semen sample analysis is routinely performed by microscopical evaluation and manual techniques by laboratory operators; the analysis is affected by a wide imprecision related to variability among observers, influencing its clinical validity. Our aim was to automate sperm analysis with the use of flow cytometry for evaluation of cell counts and typing and with the use of a new membrane-permeant nucleic acid stain for evaluation of sperm viability. Statistical analysis of the comparison between manual and automated methods for sperm counts was performed by the Bland and Altman method; the mean difference was 0.243 × 106 sperms/mL. The precision of the flow cytometric analysis was evaluated with whole sperm; the between-run CV was 7.5% and the within-run CV was 2.5%. Data observed suggest that flow cytometric sperm analysis, with high precision and accuracy and low costs, can be proposed for routine use in clinical laboratories.

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