A Highly Sensitive Enzyme Immunoassay of Anti-Insulin Antibodies in Guinea Pig Serum

SummaryTreatment of serum with bentonite led to a reduced content of inhibitors of trypsin and urokinase in the isoelectrically precipitated euglobulin, and removed fibrinolytic agents and precursors from serum. Bentonite-treated serum added to untreated serum reduced precipitation of the above inhibitors, and presumably also precipitation of inhibitors against a plasminogen activator of serum.Bentonite-treated serum (whether from pig, ox, guinea-pig, or man), added to untreated guinea-pig serum, produced fibrinolytic activity on isoelectric precipitation of the mixture; the activity of the euglobulin was due to an activator of plasminogen as well as an active protease, probably plasmin. The described effects of bentonite-treated serum are similar to those previously reported for anionic polyelectrolytes. Possible mechanisms are discussed.The “non-specific” activation of fibrinolytic activity by means of bentonite emphasizes that guinea-pig serum [which is characterized by a high potential for “nonspecific” activation of its fibrinolytic system Olesen (1962)] contains all the elements required for the formation of an activator of plasminogen, and thus the activation of its plasminogen to plasmin.

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A highly sensitive sandwich enzyme immunoassay of urinary growth hormone in children with short stature, Turner's syndrome, and simple obesity

Acta Endocrinologica ◽

10.1530/acta.0.1210513 ◽

1989 ◽

Vol 121 (4) ◽

pp. 513-519 ◽

Cited By ~ 6

Author(s):

Hiroshi Tomita ◽

Masamichi Ogawa ◽

Takashi Kamijo ◽

Osamu Mori ◽

Eiji Ishikawa ◽

...

Keyword(s):

Short Stature ◽

Enzyme Immunoassay ◽

Gh Deficiency ◽

Urine Samples ◽

Turner's Syndrome ◽

Turner’S Syndrome ◽

Highly Sensitive ◽

Significant Difference ◽

Simple Obesity ◽

Sandwich Enzyme Immunoassay

Abstract. GH values were determined by a highly sensitive sandwich enzyme immunoassay in the 1st morning and/or 24-h accumulated urine samples in 94 children (short stature 70, including 14 with complete GH deficiency, 9 with partial GH deficiency, and 47 with GH-normal short stature; Turner's syndrome, 10, and simple obesity, 14). GH values were also determined in the 2nd to 4th urine samples taken on the same day together with the 1st morning urine in 5 of them. GH values in the 1st morning urine correlated significantly with those of the 24-h urine and with serum peak and mean GH values during nocturnal sleep as a physiological GH secretion test. The 2nd to 4th urines had lower GH concentrations than the 1st morning urine. The GH value of the 1st morning urine in complete GH deficiency was significantly lower than those in GH-normal short stature, partial GH deficiency and Turner's syndrome. However, no significant difference was detected in urinary GH values between complete GH deficiency and simple obesity. We conclude that 1st morning urinary GH estimation may be useful for differentiation of complete GH deficiency from other causes of short stature, but may be difficult for the distinction between complete GH deficiency and obesity with normal GH secretory ability.

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Interactions of C-reactive protein with the complement system. III. Complement-dependent passive hemolysis initiated by CRP.

Journal of Experimental Medicine ◽

10.1084/jem.142.5.1065 ◽

1975 ◽

Vol 142 (5) ◽

pp. 1065-1077 ◽

Cited By ~ 80

Author(s):

A.P. Osmand ◽

R.F. Mortensen ◽

Joan Siegel ◽

H. Gewurz

Keyword(s):

Guinea Pig ◽

Human Serum ◽

Complete System ◽

Gel Filtration ◽

C Reactive Protein ◽

Inflammatory Reactions ◽

Reactive Protein ◽

Rabbit Igg ◽

The Complement System ◽

Pig Serum

Interactions of CRP with various substrates in the presence of human serum have been shown to result in efficient activation of C components C1-C5. We now report the ability of CRP to initiate C-dependent hemolysis. For this purpose CRP was isolated by affinity chromatography using pneumococcal CPS and gel filtration; its purity was established by several criteria. Erythrocytes were coated with CPS (E-CPS) and passively sensitized with CRP. C-dependent lysis of these cells was observed upon the addition of suitably absorbed human serum, and the efficiency of hemolysis compared favorably with that initiated by rabbit IgG anti-CPS antibody. CRP also sensitized E-CPS for lysis by guinea pig C; partial lysis was seen when C4-deficient guinea pig serum was used, suggesting that CRP also shares with antibody the ability of CRP to fully activate the C system and provide further evidence for a role for CRP similar to that of antibody in the initiation and modulation of inflammatory reactions via the complete system.

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Electrophoretic Studies of Normal Rovine and Guinea-Pig Serum

Acta Veterinaria Scandinavica ◽

10.1186/bf03547173 ◽

1963 ◽

Vol 4 (1) ◽

pp. 64-84

Author(s):

I. Koefoed Jensen

Keyword(s):

Guinea Pig ◽

Pig Serum

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