scholarly journals Engineering acidic Streptomyces rubiginosus D-xylose isomerase by rational enzyme design

2014 ◽  
Vol 27 (2) ◽  
pp. 59-64 ◽  
Author(s):  
M. J. Waltman ◽  
Z. K. Yang ◽  
P. Langan ◽  
D. E. Graham ◽  
A. Kovalevsky
Keyword(s):  
Xylose Isomerase ◽  
Enzyme Design ◽  
Streptomyces Rubiginosus

scholarly journals High-pressure small-angle neutron scattering studies of glucose isomerase conformation in solution

2009 ◽  
Vol 42 (3) ◽  
pp. 461-468 ◽  
Author(s):  
Ewa Banachowicz ◽  
Maciej Kozak ◽  
Adam Patkowski ◽  
Gerhard Meier ◽  
Joachim Kohlbrecher
Keyword(s):  
Neutron Scattering ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Electrostatic Interactions ◽  
Xylose Isomerase ◽  
Glucose Isomerase ◽  
Secondary Standard ◽  
High Structural Stability ◽  
Streptomyces Rubiginosus ◽  
Sans Data

Small-angle neutron scattering (SANS) of solutions of glucose/xylose isomerase fromStreptomyces rubiginosuswas measured as a function of pressure. It is shown that the structure of the enzyme in solution as seen by SANS is practically the same as that in the crystal and does not change with pressure up to 150 MPa. This reflects the unusually high structural stability of this material, which makes it extremely interesting to use as a secondary standard for pressure-dependent SANS experiments. This lack of pressure dependence of the SANS data also indicates that any possible change in hydration of the protein induced by pressure is not visible in the SANS curves. An appropriate correction procedure must be used for the SANS data in order to account for the distortion of the intensity curve due to hard-sphere and electrostatic interactions. After this correction, the isomerase can be readily used as a secondary standard for SANS measurements.

Novel reactions of xylose isomerase from Streptomyces rubiginosus

1999 ◽  
Vol 25 (8-9) ◽  
pp. 695-700 ◽  
Author(s):  
Ossi Pastinen ◽  
Kalevi Visuri ◽  
Hans E Schoemaker ◽  
Matti Leisola
Keyword(s):  
Xylose Isomerase ◽  
Streptomyces Rubiginosus

scholarly journals Spectroscopic studies on the metal-ion-binding sites of Co2+-substituted D-xylose isomerase from Streptomyces rubiginosus

1990 ◽  
Vol 193 (3) ◽  
pp. 863-871 ◽  
Author(s):  
Christoph SUDFELDT ◽  
Andreas SCHAFFER ◽  
Jeremias H. R. KAGI ◽  
Ralf BOGUMIL ◽  
Hans-Peter SCHULZ ◽  
...  
Keyword(s):  
Binding Sites ◽  
Metal Ion ◽  
Xylose Isomerase ◽  
Spectroscopic Studies ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Ion Binding Sites ◽  
Streptomyces Rubiginosus ◽  
Metal Ion Binding Sites

Solubility and crystallization of xylose isomerase from Streptomyces rubiginosus

2003 ◽  
Vol 257 (3-4) ◽  
pp. 403-411 ◽  
Author(s):  
Antti Vuolanto ◽  
Sinikka Uotila ◽  
Matti Leisola ◽  
Kalevi Visuri
Keyword(s):  
Xylose Isomerase ◽  
Streptomyces Rubiginosus

scholarly journals Safety evaluation of the food enzyme xylose isomerase from the genetically modified Streptomyces rubiginosus strain DP‐Pzn37

EFSA Journal ◽  
2020 ◽  
Vol 18 (1) ◽  
Author(s):  
◽  
Vittorio Silano ◽  
José Manuel Barat Baviera ◽  
Claudia Bolognesi ◽  
Pier Sandro Cocconcelli ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Safety Evaluation ◽  
Xylose Isomerase ◽  
Streptomyces Rubiginosus
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