scholarly journals Transposon impala, a Novel Tool for Gene Tagging in the Rice Blast Fungus Magnaporthe grisea

2001 ◽  
Vol 14 (3) ◽  
pp. 308-315 ◽  
Author(s):  
François Villalba ◽  
Marc-Henri Lebrun ◽  
Aurélie Hua-Van ◽  
Marie-Josée Daboussi ◽  
Marie-Claire Grosjean-Cournoyer
Keyword(s):  
Nitrate Reductase ◽  
Fusarium Oxysporum ◽  
Rice Blast ◽  
Insertional Mutagenesis ◽  
Magnaporthe Grisea ◽  
Rice Blast Fungus ◽  
Single Copy ◽  
Wild Type ◽  
Type Locus ◽  
Blast Fungus

impala, a Tc1-mariner transposable element from Fusarium oxysporum, was introduced into the rice blast fungus Magnaporthe grisea to develop transposon-based insertional mutagenesis. A construct (pNIL160) containing an autonomous impala copy inserted in the promoter of niaD encoding Aspergillus nidulans nitrate reductase was introduced by transformation into a M. grisea nitrate reductase-deficient mutant. impala excision was monitored by restoration of prototrophy for nitrate. Southern analysis of niaD+ revertants revealed that impala was able to excise and reinsert at new loci in M. grisea. As observed for its host Fusarium oxysporum, impala inserted at a TA site left a typical excision footprint of 5 bp. We have shown that a defective impala copy was inactive in M. grisea, yet it can be activated by a functional impala transposase. A transformant carrying a single copy of pNIL160 was used to generate a collection of 350 revertants. Mutants either altered for their mycelial growth (Rev2) or nonpathogenic (Rev77) were obtained. Complementation of Rev77 with a 3-kb genomic fragment from a wild-type locus was successful, demonstrating the tagging of a pathogenicity gene by impala. This gene, called ORP1, is essential for penetration of host leaves by M. grisea and has no sequence homology to known genes.

scholarly journals Mapping of Avirulence Genes in the Rice Blast Fungus, Magnaporthe grisea, with RFLP and RAPD Markers

2000 ◽  
Vol 13 (2) ◽  
pp. 217-227 ◽  
Author(s):  
Waly Dioh ◽  
Didier Tharreau ◽  
Jean Loup Notteghem ◽  
Marc Orbach ◽  
Marc-Henri Lebrun
Keyword(s):  
Genetic Map ◽  
Rice Blast ◽  
Rapd Markers ◽  
Magnaporthe Grisea ◽  
Rice Blast Fungus ◽  
Single Copy ◽  
Avirulence Gene ◽  
Rflp Markers ◽  
Avirulence Genes ◽  
Blast Fungus

Three genetically independent avirulence genes, AVR1-Irat7, AVR1-MedNoï, and AVR1-Ku86, were identified in a cross involving isolates Guy11 and 2/0/3 of the rice blast fungus, Magnaporthe grisea. Using 76 random progeny, we constructed a partial genetic map with restriction fragment length polymorphism (RFLP) markers revealed by probes such as the repeated sequences MGL/MGR583 and Pot3/MGR586, cosmids from the M. grisea genetic map, and a telomere sequence oligonucleotide. Avirulence genes AVR1-MedNoï and AVR1-Ku86 were closely linked to te-lomere RFLPs such as marker TelG (6 cM from AVR1-MedNoï) and TelF (4.5 cM from AVR1-Ku86). Avirulence gene AVR1-Irat7 was linked to a cosmid RFLP located on chromosome 1 and mapped at 20 cM from the avirulence gene AVR1-CO39. Using bulked segregant analysis, we identified 11 random amplified polymorphic DNA (RAPD) markers closely linked (0 to 10 cM) to the avirulence genes segregating in this cross. Most of these RAPD markers corresponded to junction fragments between known or new transposons and a single-copy sequence. Such junctions or the whole sequences of single-copy RAPD markers were frequently absent in one parental isolate. Single-copy sequences from RAPD markers tightly linked to avirulence genes will be used for positional cloning.

Synthesis and Biotransformation of Plausible Biosynthetic Intermediates of Salicylaldehyde-Type Phytotoxins of Rice Blast Fungus, Magnaporthe grisea

2011 ◽  
Vol 2011 (31) ◽  
pp. 6276-6280 ◽  
Author(s):  
Koji Tanaka ◽  
Ayaka Sasaki ◽  
Hai-Qun Cao ◽  
Teiko Yamada ◽  
Masahiro Igarashi ◽  
...  
Keyword(s):  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Rice Blast Fungus ◽  
Blast Fungus

Proteome analysis of rice blast fungus (Magnaporthe grisea) proteome during appressorium formation

PROTEOMICS ◽  
2004 ◽  
Vol 4 (11) ◽  
pp. 3579-3587 ◽  
Author(s):  
Sun Tae Kim ◽  
Seok Yu ◽  
Sang Gon Kim ◽  
Han Ju Kim ◽  
Sun Young Kang ◽  
...  
Keyword(s):  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Proteome Analysis ◽  
Rice Blast Fungus ◽  
Appressorium Formation ◽  
Blast Fungus

scholarly journals The Ambivalence of the Barley Mlo Locus: Mutations Conferring Resistance Against Powdery Mildew (Blumeria graminis f. sp. hordei) Enhance Susceptibility to the Rice Blast Fungus Magnaporthe grisea

1999 ◽  
Vol 12 (6) ◽  
pp. 508-514 ◽  
Author(s):  
Birgit Jarosch ◽  
Karl-Heinz Kogel ◽  
Ulrich Schaffrath
Keyword(s):  
Powdery Mildew ◽  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Durable Resistance ◽  
Rice Blast Fungus ◽  
Blumeria Graminis ◽  
Negative Regulator ◽  
Powdery Mildew Fungus ◽  
Mesophyll Cells ◽  
Blast Fungus

Recessive alleles of the barley Mlo locus confer non-race-specific resistance against the powdery mildew fungus Blumeria graminis f. sp. hordei (Bgh). Recently the Mlo gene has been isolated and it was suggested that the Mlo product is a negative regulator of cell death. Thus, loss of function can precondition cells to a higher responsiveness for the onset of multiple defense functions. Here, we document an enhanced susceptibility of barley mlo mutants to the rice blast fungus Magnaporthe grisea. The disease phenotype is independent of the barley cultivar in which the mlo allele has been introgressed and occurs in equal amounts in barley backcross lines of cv. Ingrid carrying the mlo-1, mlo-3, or mlo-5 allele. Ror genes, which are required for the full expression of mlo resistance in barley against Bgh, do not affect the specific mlo-mediated phenotype observed after M. grisea infection. Formation of an effective papilla restricts blast development in epidermal cells of Mlo plants. In contrast, papillae are mostly penetrated in mlo mutants and, as a consequence, the fungus spreads into adjacent mesophyll cells. Both wild-type plants and mlo mutants did not differ in perception of a purified elicitor derived from M. grisea. Thus, we hypothesize that a functional Mlo protein is a prerequisite for penetration resistance of barley to fungal pathogens like M. grisea. The benefit of mlo alleles for durable resistance in barley and a proposed role of mlo-type-mutations in rice are discussed.

Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance

1990 ◽  
Vol 17 (5) ◽  
pp. 409-411 ◽  
Author(s):  
Hei Leung ◽  
Ulla Lehtinen ◽  
Reijo Karjalainen ◽  
Daniel Skinner ◽  
Paul Tooley ◽  
...  
Keyword(s):  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Rice Blast Fungus ◽  
Hygromycin B ◽  
Hygromycin B Resistance ◽  
Blast Fungus
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