scholarly journals First Report of Apple stem grooving virus, Apple stem pitting virus, and Apple chlorotic leaf spot virus in Apple Trees From Chencha, Ethiopia

Plant Disease ◽  
2016 ◽  
Vol 100 (12) ◽  
pp. 2540 ◽  
Author(s):  
B. Lemma ◽  
F. Xing ◽  
S. F. Li ◽  
Z. X. Zhang
Keyword(s):  
Leaf Spot ◽  
Apple Trees ◽  
Spot Virus ◽  
First Report ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf ◽  
Apple Stem Pitting Virus ◽  
Stem Pitting

scholarly journals Gospodarski važni virusi jezgričavog voća

2019 ◽  
Vol 22 (3-4) ◽  
pp. 123-136
Author(s):  
Dario Ivić
Keyword(s):  
Mosaic Virus ◽  
Leaf Spot ◽  
Spot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf ◽  
Apple Stem Pitting Virus ◽  
Stem Pitting

Virusi jabuke, kruške ili dunje relativno su slabo poznati stručnjacima i voćarima. Najvažnijim virusima koji se javljaju na jezgričavim voćnim vrstama smatraju se virus mozaika jabuke (Apple mosaic virus, ApMV), virus klorotične pjegavosti lista jabuke (Apple chlorotic leaf spot virus, ACLSV), virus brazdavosti debla jabuke (Apple stem grooving virus, ASGV) i virus jamičavosti debla jabuke (Apple stem pitting virus,ASPV). U radu je ukratko opisana njihova važnost, biologija i regulativni status, kao i osnovne mjere zaštite.

scholarly journals First Report of Apple Top Working Disease Caused by Viruses (Apple stem grooving virus, Apple chlorotic leaf spot virus, and Apple stem pitting virus) in Apple in India

Plant Disease ◽  
2013 ◽  
Vol 97 (7) ◽  
pp. 1001-1001 ◽  
Author(s):  
A. Brakta ◽  
P. D. Thakur ◽  
A. Handa
Keyword(s):  
Leaf Spot ◽  
Spot Virus ◽  
Sequence Identity ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Commercial Cultivars ◽  
Chlorotic Leaf ◽  
Apple Stem Pitting Virus ◽  
Stem Pitting ◽  
Das Elisa

Top working of apple (Malus domestica Borkh.) trees of old, unproductive, and less preferred cultivars with the newly introduced spur type commercial cultivars has become a common practice with many growers in the northwestern Himalayan region of India. Typical viral symptoms of curling, puckering, and necrosis on leaves were observed with an incidence of 80% on Red Chief, Super Chief, Scarlet Spur, Schillet Spur, Washington Red Delicious, and many other newly introduced cultivars during surveys conducted in May and June 2009. Leaf samples from top worked trees were tested for the presence of Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), and Apple stem pitting virus (ASPV) by employing biological detection (herbaceous and woody indicators), double antibody sandwich (DAS)-ELISA), and reverse transcriptase (RT)-PCR based detection. Mechanical transmission to herbaceous indicators produced chlorotic lesions on Chenopodium quinoa and C. amaranticolor, whereas marginal necrosis was induced on Phaseolous vulgaris within 9 to 21 days after sap inoculations. All three viruses, i.e., ASGV, ASPV, and ACLSV, were detected from these herbaceous indicators in DAS-ELISA (BIOREBA AG, Switzerland). Furthermore, symptoms similar to those observed in orchards were produced when the test budwood was inoculated onto the woody indicator (M. pumila ‘Spy 227’) plant by double grafting, grafting cum budding, and double budding methods within time periods ranging from 4 months in double grafting, 5 months in double budding, to 1 year 4 months in the grafting cum budding method. The presence of all three viruses was confirmed by DAS-ELISA again in Spy 227 woody indicator. PCR detection was carried out by using the coat protein gene specific primers (ASGV5641 [forward], ASGV6396 [reverse]; ACLSV6784 [forward], ACLSV7365 [reverse] [2]; ASP-C [sense], ASP-A [anti-sense] [1]) of all the viruses detected through ELISA. The amplified products were cloned, sequenced, and deposited in NCBI (GenBank Accessions KC110892 for ASGV, KC154859 for ASPV, and KC154862 for ACLSV). BLASTn analysis showed the ASGV isolate had 97 to 98% sequence identity with Indian (FM204881) and Brazilian (AF438409) ASGV isolates. The ASPV and ACLSV isolates had 98% and 99% sequence identity with Chinese (JF895517) and Japanese (AB326230) isolates, respectively. To the best of our knowledge, this is the first report of apple top working disease associated with ASGV, ASPV, and ACLSV infection in commercial cultivars of apple from India and seems to be a serious threat for growing virus-free healthy stocks in orchards. Top working disease in apple associated with ASGV, ASPV, and ACLSV viruses has been reported from Japan (3,4). References: (1) J. K. Kundu et al. Plant Prot. Sci. 39:88, 2003. (2) O. Nickel et al. Fitopatol. Brasil. 26:655, 2001. (3) H. Yanase. Bull. Fruit Tree Res. Stn., Japan Ser. C 1:47, 1974. (4) H. Yanase et al. Acta Hortic. 44:221, 1975.

scholarly journals Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe

2014 ◽  
Vol 44 (10) ◽  
pp. 1711-1714 ◽  
Author(s):  
Thor Vinícius Martins Fajardo ◽  
Osmar Nickel
Keyword(s):  
Leaf Spot ◽  
Simultaneous Detection ◽  
Hybridization Method ◽  
Spot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf ◽  
Viral Cdna ◽  
Apple Stem Pitting Virus ◽  
Stem Pitting

The viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was sufficiently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specificity was confirmed by reaction with homologous viral cDNA, individually cloned for each virus.

scholarly journals Survey of Apple Chlorotic Leaf Spot Virus and Apple Stem Grooving Virus Occurrence in Korea and Frequency of Mixed Infections in Apple

2015 ◽  
Vol 60 (2) ◽  
pp. 323-329 ◽  
Author(s):  
Jae-Yeong Han ◽  
Jung-Kyu Kim ◽  
Jin–Soo Cheong ◽  
Eun–Yeong Seo ◽  
Chan–Hwan Park ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Mixed Infections ◽  
Spot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf

Distribution of apple stem grooving virus and apple chlorotic leaf spot virus in infected in vitro pear shoots

2010 ◽  
Vol 29 (12) ◽  
pp. 1447-1451 ◽  
Author(s):  
L.P. Wang ◽  
N. Hong ◽  
G.P. Wang ◽  
W.X. Xu ◽  
R. Michelutti ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Spot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf

scholarly journals Survey for viruses and viroids in apple and pear trees in New Zealand

2010 ◽  
Vol 63 ◽  
pp. 281-281
Author(s):  
M.B. Horner
Keyword(s):  
New Zealand ◽  
Spot Virus ◽  
Tomato Ringspot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf ◽  
Pear Trees ◽  
Leaf Virus ◽  
Stem Pitting ◽  
Geographical Locations

Surveys to determine the phytosanitary status of apple (Malus) and pear (Pyrus) trees in New Zealand were conducted from 2005 to 2006 A total of 188 symptomatic and nonsymptomatic trees from various geographical locations were tested for the presence of a number of viruses and viroids by RTPCR All Malus samples were tested for Apple chlorotic leaf spot virus (ACLSV) Apple stem grooving virus (ASGV) Apple stem pitting virus (ASPV) Apple mosaic virus (ApMV) Apple scar skin viroid (ASSVd) Apple dimple fruit viroid (ADFVd) Cherry rasp leaf virus (CRLV) and Tomato ringspot virus (ToRSV) ACLSV was detected in 48 ASGV in 36 ASPV in 61 and ApMV in 45 of samples tested ASSVd ADFVd CRLV ToRSV were not detected in any of the 165 sampled Malus plants which provides evidence that they are not present in New Zealand All Pyrus samples were tested for ACLSV ASGV ASPV ApMV Pear latent virus (PeLV) and Pear blister canker viroid (PBCVd) ACLSV was detected in 17 ASGV in 13 ASPV in 17 and ApMV in 4 of sampled Pyrus trees PeLV and PBCVd were not detected in any of the 23 sampled symptomatic Pyrus trees which provides evidence that PeLV and PBCVd are not present in New Zealand

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