Characterization of PmDGM conferring powdery mildew resistance in Chinese wheat landrace Duanganmang

Plant Disease ◽  
2021 ◽  
Author(s):  
Yanan Wu ◽  
Xiaoting Yu ◽  
Xu Zhang ◽  
Lijuan Yan ◽  
Li Gao ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Single Nucleotide Polymorphism Array ◽  
Single Nucleotide ◽  
Mildew Resistance ◽  
Wheat Landrace ◽  
Yield And Quality ◽  
Genome Homology ◽  
Chinese Wheat

Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease threatening yield and quality. Host resistance is considered the most effective and preferred means to control this disease. Wheat landrace Duanganmang (DGM) showed high resistance or near immunity to Bgt mixture from Henan province, China. DGM was crossed with highly susceptible Chinese wheat landrace Huixianhong (HXH) and cultivar Shimai 15 (SM15) to produce genetic populations. The resistance of DGM to Bgt isolate E09 was shown to be controlled by a single dominant Mendelian factor, tentatively designated PmDGM. Marker analysis and 55K SNP (single nucleotide polymorphism) array scanning showed that this gene was positioned in the Pm5 interval (2.4 cM or 1.61 Mb) flanked by Xhenu099 and Xmp1158 in the Chinese Spring reference genome. Homology-based cloning and sequence analysis demonstrated that DGM has the identical NLR gene (Pm5e) and RXL gene reported in Fuzhuang 30 (FZ30) conferring and modifying the powdery mildew resistance, respectively. However, based on the different reaction patterns to the Bgt isolate B15 between DGM and FZ30, we speculate that DGM may have two tightly linked genes that could not be separated in the current mapping population, one is PmDGM and the other is Pm5e. Hence, this study provides a valuable resistance resource for improvement of powdery mildew resistance.

Characterization of Pm59, a novel powdery mildew resistance gene in Afghanistan wheat landrace PI 181356

2018 ◽  
Vol 131 (5) ◽  
pp. 1145-1152 ◽  
Author(s):  
Chengcheng Tan ◽  
Genqiao Li ◽  
Christina Cowger ◽  
Brett F. Carver ◽  
Xiangyang Xu
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Wheat Landrace

scholarly journals Identification of a New Powdery Mildew Resistance Gene pmDHT at or Closely Linked to the Pm5 Locus in the Chinese Wheat Landrace Dahongtou

Plant Disease ◽  
2019 ◽  
Vol 103 (10) ◽  
pp. 2645-2651 ◽  
Author(s):  
Yanmin Qie ◽  
Yuan Sheng ◽  
Hongxing Xu ◽  
Yuli Jin ◽  
Feifei Ma ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Recessive Gene ◽  
Genetic Distances ◽  
Linked Gene ◽  
Mildew Resistance ◽  
Wheat Landrace ◽  
Allelism Tests ◽  
Wheat Lines ◽  
Chinese Wheat

Chinese wheat landrace Dahongtou was resistant to 35 of 38 tested Chinese isolates of Blumeria graminis f. sp. tritici at the seedling stage. Genetic analysis of the F2 populations and their derived F2:3 families of crosses of Dahongtou with the susceptible varieties Mingxian 169 and Huixianhong indicated that the resistance of Dahongtou to B. graminis f. sp. tritici isolate E09 was conferred by a single recessive gene, tentatively designated as pmDHT. The gene was mapped to chromosome arm 7BL and flanked by markers Xwmc526/XBE443877 and Xgwm611/Xwmc511 at genetic distances of 0.8 and 0.3 cM, respectively. The chromosomal position of pmDHT was similar to the multi-allelic Pm5 locus on 7BL. Allelism tests with crosses of Dahongtou with Fuzhuang 30 (Pm5e) and Xiaobaidong (mlxbd) indicated that pmDHT was allelic to both Pm5e and mlxbd. However, pmDHT showed a different pattern of resistance to the 38 B. graminis f. sp. tritici isolates compared with wheat lines with Pm5a, Pm5b, Pm5e, mlxbd, and PmHYM and also differed from PmSGA. Thus, pmDHT was identified most likely as a new allele or at least a closely linked gene of the Pm5 locus. This gene can be transferred into susceptible wheat cultivars/lines and pyramided with other resistance genes through marker-assisted selection to improve powdery mildew resistance.

scholarly journals Mapping Powdery Mildew Resistance Gene pmYBL on Chromosome 7B of Chinese Wheat (Triticum aestivum L.) Landrace Youbailan

Plant Disease ◽  
2020 ◽  
Vol 104 (9) ◽  
pp. 2411-2417
Author(s):  
Xiaodan Xu ◽  
Wei Liu ◽  
Zhiyong Liu ◽  
Jieru Fan ◽  
Yilin Zhou
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Recessive Gene ◽  
Triticum Aestivum L ◽  
Genetic Distances ◽  
Genomic Region ◽  
Snp Markers ◽  
Single Nucleotide ◽  
Mildew Resistance ◽  
Chinese Wheat

Chinese wheat landrace Youbailan has excellent resistance to powdery mildew caused by Blumeria graminis f. sp. tritici. In the present study, genetic analysis indicated that a recessive gene, tentatively designated pmYBL, was responsible for the powdery mildew resistance of Youbailan. pmYBL was located in the 695-to-715-Mb genomic region of chromosome 7BL, with 19 gene-linked single-nucleotide polymorphism (SNP) markers. It was flanked by SNP1-12 and SNP1-2 with genetic distances of 0.6 and 1.8 centimorgans, respectively. The disease reaction patterns of Youbailan and four cultivars (lines) carrying the powdery mildew (Pm) genes located on chromosome arm 7BL indicated that pmYBL may be allelic or closely linked to these genes. All of the SNP markers linked to pmYBL were diagnostic, indicating that these markers will be useful for pyramiding pmYBL using marker-assisted selection.

Identification and mapping of a new powdery mildew resistance allele in the Chinese wheat landrace Hongyoumai

2017 ◽  
Vol 37 (11) ◽  
Author(s):  
Bisheng Fu ◽  
Zhiliang Zhang ◽  
Qiaofeng Zhang ◽  
Xiaoyou Wu ◽  
Jizhong Wu ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Resistance Allele ◽  
Mildew Resistance ◽  
Wheat Landrace ◽  
Chinese Wheat

Characterization of PmHHXM, a New Broad-spectrum Powdery Mildew Resistance Gene in Chinese Wheat Landrace Honghuaxiaomai

Plant Disease ◽  
2021 ◽  
Author(s):  
Shulin Xue ◽  
Mingxue Lu ◽  
Shanshan Hu ◽  
Hongxing Xu ◽  
Yuyu Ma ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Broad Spectrum ◽  
Powdery Mildew Resistance ◽  
Dominant Gene ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Wheat Landrace ◽  
Chinese Wheat

Powdery mildew, caused by fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is one of agronomically important and widespread wheat diseases causing severe yield losses. Deployment of broad‐spectrum disease-resistance genes is the preferred strategy to prevent this pathogen. Chinese wheat landrace Honghuaxiaomai (HHXM) was resistant to all 23 tested Bgt isolates at the seedling stage. The F1, F2, and F2:3 progenies derived from the cross HHXM × Yangmai 158 were used in this study, and genetic analysis revealed that a single dominant gene, designated as PmHHXM, conferred resistance to Bgt isolate E09. Bulked segregant analysis and molecular mapping initially located PmHHXM to the distal region of chromosome 4AL. To fine map PmHHXM, two critical recombinants were identified from 592 F2 plants and delimited PmHHXM to a 0.18-cM Xkasp475200–Xhnu552 interval covering 1.77-Mb, in which a number of disease resistance-related gene clusters were annotated. Comparative mapping of this interval revealed a perturbed synteny among Triticeae species. This study reports the new powdery mildew resistance gene PmHHXM that seems different from three known QTL/genes identified on chromosome 4AL and has significant values for further genetic improvement. Analysis of the polymorphisms of 13 co-segregating markers between HHXM and 170 modern wheat cultivars indicates that Xhnu227 and Xsts478700 developed here are ideal for marker-assisted introgression of this resistance gene in wheat breeding.

Characterization of the Powdery Mildew Resistance Gene in Wheat Breeding Line KN0816 and its Evaluation in Marker-Assisted Selection

Plant Disease ◽  
2021 ◽  
Author(s):  
Wenrui Wang ◽  
Huagang He ◽  
Huiming Gao ◽  
Hongxing Xu ◽  
Wenyue Song ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Marker Assisted Selection ◽  
Powdery Mildew Resistance ◽  
Wheat Breeding ◽  
Agronomic Performance ◽  
Breeding Line ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance

Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease seriously threatening yield and quality of common wheat (Triticum aestivum L., 2n=6x=42, AABBDD). Characterization of resistance genes against powdery mildew is useful in parental selection and for developing disease resistant cultivars. Chinese wheat breeding line KN0816 has superior agronomic performance and resistance to powdery mildew at all growth stages. Genetic analysis using populations of KN0816 crossed with different susceptible parents indicated that a single dominant gene, tentatively designated PmKN0816, conferred seedling resistance to different Bgt isolates. Using a bulked segregant analysis (BSA), PmKN0816 was mapped to the Pm6 interval on chromosome arm 2BL using polymorphic markers linked to the catalogued genes Pm6, Pm52, and Pm64, and flanked by markers CISSR02g-6 and CIT02g-2 both with genetic distances of 0.7 cM. Analysis of closely linked molecular markers indicated that the marker alleles of PmKN0816 differed from those of other powdery mildew resistance genes on 2BL, including Pm6, Pm33, Pm51, Pm64, and PmQ. Based on the genetic and physical locations and response pattern to different Bgt isolates, PmKN0816 is most likely a new powdery mildew resistance gene and confers effective resistance to all the 14 tested Bgt isolates. In view of the elite agronomic performance of KN0816 combined with the resistance, PmKN0816 is expected to become a valuable resistance gene in wheat breeding. To transfer PmKN0816 to different genetic backgrounds using marker-assisted selection (MAS), closely linked markers of PmKN0816 were evaluated and four of them (CIT02g-2, CISSR02g-6, CIT02g-10, and CIT02g-17) were confirmed to be applicable for MAS in different genetic backgrounds.

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