scholarly journals The CYP4A‐20‐HETE System in Regulation of Endothelial Progenitor Cell Functions

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
austin meng guo ◽  
Branislave Janic ◽  
Ali S Arbab ◽  
Paul A Edwards ◽  
Alfonso Guillermo Scicli
Keyword(s):  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Endothelial Progenitor ◽  
Cell Functions

scholarly journals Phosphoinositide 3-Kinase γ Gene Knockout Impairs Postischemic Neovascularization and Endothelial Progenitor Cell Functions

2008 ◽  
Vol 28 (1) ◽  
pp. 68-76 ◽  
Author(s):  
Paolo Madeddu ◽  
Nicolle Kraenkel ◽  
Luciola S. Barcelos ◽  
Mauro Siragusa ◽  
Paola Campagnolo ◽  
...  
Keyword(s):  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Gene Knockout ◽  
Endothelial Progenitor ◽  
Cell Functions ◽  
Phosphoinositide 3 Kinase

Niacin improves ischemia-induced neovascularization in diabetic mice by enhancement of endothelial progenitor cell functions independent of changes in plasma lipids

Angiogenesis ◽  
2012 ◽  
Vol 15 (3) ◽  
pp. 377-389 ◽  
Author(s):  
Po-Hsun Huang ◽  
Chih-Pei Lin ◽  
Chao-Hung Wang ◽  
Chia-Hung Chiang ◽  
Hsiao-Ya Tsai ◽  
...  
Keyword(s):  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Plasma Lipids ◽  
Diabetic Mice ◽  
Endothelial Progenitor ◽  
Cell Functions

scholarly journals Far infra-red therapy promotes ischemia-induced angiogenesis in diabetic mice and restores high glucose-suppressed endothelial progenitor cell functions

2012 ◽  
Vol 11 (1) ◽  
pp. 99 ◽  
Author(s):  
Po-Hsun Huang ◽  
Jaw-Wen Chen ◽  
Chih-Pei Lin ◽  
Ying-Hwa Chen ◽  
Chao-Hung Wang ◽  
...  
Keyword(s):  
High Glucose ◽  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Diabetic Mice ◽  
Endothelial Progenitor ◽  
Cell Functions ◽  
Infra Red

scholarly journals Glycosaminoglycan mimetic improves enrichment and cell functions of human endothelial progenitor cell colonies

2014 ◽  
Vol 12 (3) ◽  
pp. 703-715 ◽  
Author(s):  
Fabien Chevalier ◽  
Mélanie Lavergne ◽  
Elisa Negroni ◽  
Ségolène Ferratge ◽  
Gilles Carpentier ◽  
...  
Keyword(s):  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Endothelial Progenitor ◽  
Cell Functions

Abstract 40: Interleukin-10 Deficiency Impairs Reparative Properties of Bone Marrow-derived Endothelial Progenitor Cell Exosomes Function in Ischemic Myocardium

2016 ◽  
Vol 119 (suppl_1) ◽  
Author(s):  
Yujia Yue ◽  
Venkata N Garikipati ◽  
Yan Tang ◽  
Zhongjian Chen ◽  
Jibin Zhou ◽  
...  
Keyword(s):  
Stem Cell ◽  
Systemic Inflammation ◽  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Interleukin 10 ◽  
Ischemic Myocardium ◽  
Common Symptom ◽  
Endothelial Progenitor ◽  
Cell Functions

Endothelial progenitor cell (EPC) based therapy promotes neovascularization in ischemic myocardium. Studies suggest that therapeutic effect of stem cells is largely due to paracrine mechanisms including stem cell-derived exosomes that are emerging as key paracrine mediators of stem cell functions. However, the autologous transplantation of EPCs in patients with systemic inflammation, which is a common symptom in patients with ischemic heart diseases, yield modest results suggesting the compromised cell function and altered exosome performance. We hypothesized that EPCs under inflammatory stress secrete dysfunctional exosomes with altered contents including packaged microRNAs, which eventually compromises exosomes repair ability in ischemic myocardium. Whether modulation of identified targets like specific microRNAs in the cargo of exosomes can rescue and/or enhance their reparative properties of dysfunctional exosomes is not known. We have previously shown in Interleukin-10 (IL-10) KO mice (model mimicking systemic inflammation) that loss of IL-10 impairs EPCs functions via miR-375. After cell expansion, we isolated exosomes from these two groups and compared their functions in terms of cell survival, proliferation, migration and angiogenic capacity in vitro and in vivo . We report that WT-EPC-exosomes (Exo) transplantation in the ischemic myocardium after MI significantly improved post-infarct repair, neovascularization and left ventricle functions. Our in vitro studies revealed that WT-EPC-Exo treatment enhanced endothelial cells proliferation and tube formation and inhibited apoptosis; whereas IL-10 KO-EPC-Exo exhibited opposite effects, suggesting that reparative capacity of WT-EPC-Exo is lost in exosomes derived from IL-10 KO-EPCs. Exosome miRNA profiling revealed a drastically different expression patterns with enrichment of inflammation induced microRNAs including significantly higher expression of miR-375. Interestingly, Knockdown of miR-375 in IL-10-EPCs decreased the expression level of miR-375 in their exosomes and partly rescued their angiogenic dysfunctions. Taken together, our studies suggest that modulation of miR-375 in IL-10 KO-EPC-Exo can rescue its phenotype and promote cardiac repair.

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