scholarly journals High Resolution Field Cycling for Analysis of Lipid Dynamics in Membranes

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Mary Fedarko Roberts ◽  
Mingming Pu ◽  
Alfred G. Redfield
Keyword(s):  
High Resolution ◽  
Lipid Dynamics ◽  
Field Cycling

Phospholipids in Motion: High-Resolution 31P NMR Field Cycling Studies

2021 ◽  
Author(s):  
Mary F. Roberts ◽  
Jingfei Cai ◽  
Sivanandam V. Natarajan ◽  
Hanif M. Khan ◽  
Nathalie Reuter ◽  
...  
Keyword(s):  
High Resolution ◽  
31P Nmr ◽  
Field Cycling

scholarly journals High Resolution Field Cycling NMR Relaxometry Identifies Novel Phospholipid Binding Sites on PTEN and the Akt1 PH Domain

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Mary Roberts ◽  
Boguslaw Stec ◽  
Cheryl Gradziel ◽  
Yang Wei ◽  
Alfred Redfield
Keyword(s):  
High Resolution ◽  
Binding Sites ◽  
Ph Domain ◽  
Phospholipid Binding ◽  
Nmr Relaxometry ◽  
Field Cycling

scholarly journals Phospholipids in motion: High Resolution 31P NMR Field Cycling Studies

2021 ◽  
Author(s):  
Mary Roberts ◽  
Jingfei Cai ◽  
V.N. Sivanandam ◽  
Hanif M. Khan ◽  
Nathalie Reuter ◽  
...  
Keyword(s):  
High Resolution ◽  
High Field ◽  
Chemical Shift Anisotropy ◽  
Lateral Diffusion ◽  
Head Group ◽  
Polar Head Group ◽  
Field Range ◽  
Spin Lattice ◽  
Group Motion ◽  
Field Cycling

High resolution field cycling <sup>31</sup>P NMR spin-lattice relaxometry, where the sample is excited at high field, shuttled in the magnet bore for low field relaxation, then shuttled back to high field for readout of the residual magnetization, provides data on phospholipid dynamics and structure. In the field range from 11.74 down to 0.003 T three dipolar nuclear magnetic relaxation dispersions (NMRDs) and one due to <sup>31</sup>P chemical shift anisotropy contribute to R<sub>1 </sub>of phospholipids<sub>.</sub> Extraction of correlation times and maximum relaxation amplitudes for these NMRDs provides (1) lateral diffusion constants for different phospholipids in the same bilayer (illustrated with phospholipase C binding), (2) estimates of how additives alter the motion of the phospholipid about its long axis (looking at cholesterol effects), and (3) an average <sup>31</sup>P – <sup>1</sup>H angle with respect to the bilayer normal, which reveals that polar head group motion is not restricted on a µs timescale. Although this deals exclusively with phospholipids in small unilamellar vesicles, these same NMRDs can be measured for phospholipids in micelles and nanodiscs, making this technique useful for monitoring lipid behavior in a variety of structures.

High-Resolution Field-Cycling NMR Studies of a DNA Octamer as a Probe of Phosphodiester Dynamics and Comparison with Computer Simulation†

Biochemistry ◽  
2004 ◽  
Vol 43 (12) ◽  
pp. 3637-3650 ◽  
Author(s):  
Mary F. Roberts ◽  
Qizhi Cui ◽  
Christopher J. Turner ◽  
David A. Case ◽  
Alfred G. Redfield
Keyword(s):  
Computer Simulation ◽  
High Resolution ◽  
Nmr Studies ◽  
Field Cycling

scholarly journals Enzyme–Substrate–Cofactor Dynamical Networks Revealed by High-Resolution Field Cycling Relaxometry

Biochemistry ◽  
2020 ◽  
Vol 59 (25) ◽  
pp. 2359-2370
Author(s):  
Masha M. Rosenberg ◽  
Tianjiong Yao ◽  
Gregory C. Patton ◽  
Alfred G. Redfield ◽  
Mary F. Roberts ◽  
...  
Keyword(s):  
High Resolution ◽  
Dynamical Networks ◽  
Enzyme Substrate ◽  
Field Cycling

scholarly journals High‐Resolution 31P Field Cycling NMR as a Probe of Substrates Dynamics on GMP Reductase

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Masha Rosenberg ◽  
Alfred Redfield ◽  
Mary Roberts ◽  
Lizbeth Hedstrom
Keyword(s):  
High Resolution ◽  
Field Cycling

scholarly journals Phospholipid Reorientation at the Lipid/Water Interface Measured by High Resolution 31P Field Cycling NMR Spectroscopy

2009 ◽  
Vol 97 (1) ◽  
pp. 132-141 ◽  
Author(s):  
Mary F. Roberts ◽  
Alfred G. Redfield ◽  
Udayan Mohanty
Keyword(s):  
Nmr Spectroscopy ◽  
High Resolution ◽  
Water Interface ◽  
Field Cycling

Low-Field, Time-Resolved Dynamic Nuclear Polarization with Field Cycling and High-Resolution NMR Detection

2009 ◽  
Vol 37 (1-4) ◽  
pp. 515-537 ◽  
Author(s):  
S. E. Korchak ◽  
A. S. Kiryutin ◽  
K. L. Ivanov ◽  
A. V. Yurkovskaya ◽  
Yu. A. Grishin ◽  
...  
Keyword(s):  
High Resolution ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Time Resolved ◽  
Low Field ◽  
High Resolution Nmr ◽  
Field Cycling
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