scholarly journals Sphingosine‐1‐phosphate stimulation of bladder smooth muscle contraction and signaling

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Derek M Kendig ◽  
Alec Matsumoto ◽  
Robert S. Moreland
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Smooth Muscle Contraction ◽  
Bladder Smooth Muscle ◽  
Sphingosine 1 Phosphate ◽  
Stimulation Of

scholarly journals In vivoroles for myosin phosphatase targeting subunit-1 phosphorylation sites T694 and T852 in bladder smooth muscle contraction

2014 ◽  
Vol 593 (3) ◽  
pp. 681-700 ◽  
Author(s):  
Cai-Ping Chen ◽  
Xin Chen ◽  
Yan-Ning Qiao ◽  
Pei Wang ◽  
Wei-Qi He ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Smooth Muscle Contraction ◽  
Phosphorylation Sites ◽  
Bladder Smooth Muscle ◽  
Myosin Phosphatase

The Effect of Ovariectomy and Estradiol on Rabbit Bladder Smooth Muscle Contraction and Morphology

2003 ◽  
Vol 170 (2) ◽  
pp. 634-637 ◽  
Author(s):  
KEN AIKAWA ◽  
TAKASHI SUGINO ◽  
SEIJI MATSUMOTO ◽  
PAUL CHICHESTER ◽  
CATHERINE WHITBECK ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Smooth Muscle Contraction ◽  
Bladder Smooth Muscle ◽  
Rabbit Bladder

Modulation of rate at which serotonin-induced contraction decays in guinea pig trachea

1994 ◽  
Vol 266 (1) ◽  
pp. R221-R227 ◽  
Author(s):  
R. R. Ben-Harari ◽  
B. A. Dalton ◽  
U. C. Garg
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Muscle Contraction ◽  
Airway Smooth Muscle ◽  
Smooth Muscle Contraction ◽  
Receptor Activation ◽  
Biochemical Pathways ◽  
Biochemical State ◽  
Stimulation Of

Stimulation of the type 2 serotonin (5-HT2) receptor in guinea pig trachea with 5-HT results in a contraction that decays in the continued presence of 5-HT. The decay of the 5-HT contraction has been proposed to be dependent on 5-HT2 receptor activation and to reflect desensitization of the receptor. The characteristics of the decay of the 5-HT contraction may also be dependent on other properties of the tissue. The effects of modulation of biochemical pathways implicated in airway smooth muscle contraction on the 5-HT contraction in isolated guinea pig trachea were determined with the use of a kinetic approach we developed previously. Decay of the 5-HT contraction was inhibited by cooling, increased by forskolin, 3-isobutylmethyl-1-xanthine, and 8-bromoadenosine 3',5'-cyclic monophosphate, and unaffected by staurosporine, H-7, H-8, phorbol 12,13-dibutyrate, and by inhibitors of the three major pathways of arachidonic metabolism. The results suggest that decay of the 5-HT contraction in guinea pig trachea is dependent on both the receptor and the biochemical state of the tissue.

Downregulation of profilin with antisense oligodeoxynucleotides inhibits force development during stimulation of smooth muscle

2003 ◽  
Vol 285 (4) ◽  
pp. H1528-H1536 ◽  
Author(s):  
Dale D. Tang ◽  
Jian Tan
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Actin Polymerization ◽  
Regulatory Protein ◽  
Smooth Muscle Contraction ◽  
Antisense Oligodeoxynucleotides ◽  
Nonmuscle Cells ◽  
Reversible Permeabilization ◽  
Or Organization ◽  
Stimulation Of

The actin-regulatory protein profilin has been shown to regulate the actin cytoskeleton and the motility of nonmuscle cells. To test the hypothesis that profilin plays a role in regulating smooth muscle contraction, profilin antisense or sense oligodeoxynucleotides were introduced into the canine carotid smooth muscle by a method of reversible permeabilization, and these strips were incubated for 2 days for protein downregulation. The treatment of smooth muscle strips with profilin antisense oligodeoxynucleotides inhibited the expression of profilin; it did not influence the expression of actin, myosin heavy chain, and metavinculin/vinculin. Profilin sense did not affect the expression of these proteins in smooth muscle tissues. Force generation in response to stimulation with norepinephrine or KCl was significantly lower in profilin antisense-treated muscle strips than in profilin sense-treated strips or in muscle strips not treated with oligodeoxynucleotides. The depletion of profilin did not attenuate increases in phosphorylation of the 20-kDa regulatory light chain of myosin (MLC20) in response to stimulation with norepinephrine or KCl. The increase in F-actin/G-actin ratio during contractile stimulation was significantly inhibited in profilin-deficient smooth muscle strips. These results suggest that profilin is a necessary molecule of signaling cascades that regulate carotid smooth muscle contraction, but that it does not modulate MLC20 phosphorylation during contractile stimulation. Profilin may play a role in the regulation of actin polymerization or organization in response to contractile stimulation of smooth muscle.

The role of sphingosine-1-phosphate in smooth muscle contraction

2005 ◽  
Vol 17 (3) ◽  
pp. 289-298 ◽  
Author(s):  
Kenneth R. Watterson ◽  
Paul H. Ratz ◽  
Sarah Spiegel
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Smooth Muscle Contraction ◽  
Sphingosine 1 Phosphate
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