Modulation of rate at which serotonin-induced contraction decays in guinea pig trachea

1994 ◽  
Vol 266 (1) ◽  
pp. R221-R227 ◽  
Author(s):  
R. R. Ben-Harari ◽  
B. A. Dalton ◽  
U. C. Garg
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Muscle Contraction ◽  
Airway Smooth Muscle ◽  
Smooth Muscle Contraction ◽  
Receptor Activation ◽  
Biochemical Pathways ◽  
Biochemical State ◽  
Stimulation Of

Stimulation of the type 2 serotonin (5-HT2) receptor in guinea pig trachea with 5-HT results in a contraction that decays in the continued presence of 5-HT. The decay of the 5-HT contraction has been proposed to be dependent on 5-HT2 receptor activation and to reflect desensitization of the receptor. The characteristics of the decay of the 5-HT contraction may also be dependent on other properties of the tissue. The effects of modulation of biochemical pathways implicated in airway smooth muscle contraction on the 5-HT contraction in isolated guinea pig trachea were determined with the use of a kinetic approach we developed previously. Decay of the 5-HT contraction was inhibited by cooling, increased by forskolin, 3-isobutylmethyl-1-xanthine, and 8-bromoadenosine 3',5'-cyclic monophosphate, and unaffected by staurosporine, H-7, H-8, phorbol 12,13-dibutyrate, and by inhibitors of the three major pathways of arachidonic metabolism. The results suggest that decay of the 5-HT contraction in guinea pig trachea is dependent on both the receptor and the biochemical state of the tissue.

Blockade of eosinophil migration by 5-lipoxygenase and cyclooxygenase inhibition in explanted guinea pig trachealis

1995 ◽  
Vol 268 (3) ◽  
pp. L446-L454 ◽  
Author(s):  
N. M. Munoz ◽  
A. R. Leff
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Muscle Contraction ◽  
Airway Smooth Muscle ◽  
Smooth Muscle Contraction ◽  
Cyclooxygenase Inhibition ◽  
Airway Narrowing ◽  
Eosinophil Migration ◽  
Eosinophil Chemotaxis ◽  
Physical Consequences

We studied the effects of 5-lipoxygenase inhibition with A63162 and cyclooxygenase inhibition with indomethacin (INDO) on 1) eosinophil chemotaxis and 2) airway narrowing caused by 10(-6) M formyl-Met-Leu-Phe (fMLP) in tracheal explants from guinea pigs. Airway narrowing was assessed by calibrated micrometry, and eosinophil migration from the lamina propria was expressed as number of eosinophils contained per 1 cm tracheal segment. After 120 min, treatment with fMLP caused an increase in luminal eosinophils from 6,804 +/- 1,786 to 303,347 +/- 75,609 cells (P < 0.001); airway diameter narrowed by 20.4 +/- 1.4%. In six preparations, A63162 inhibited airway narrowing caused by fMLP by 54.9 +/- 6.1%; INDO had a similar effect on airway diameter. However, maximal inhibition of eosinophil migration was greater after 10(-6) M A63162 (38,393 +/- 7,434 cells; P < 0.001 vs. fMLP alone) than after treatment with 10(-5) M INDO (123,547 +/- 19,499 cells; P < 0.05). We demonstrate a method that permits simultaneous measurements of eosinophil migration and airway smooth muscle contraction in a guinea pig tracheal explant preparation. Our data suggest that eosinophil chemotaxis and changes in internal airway diameter are caused by activation of both 5-lipoxygenase and cyclooxygenase pathways and that cell migration is independent of the physical consequences of airway smooth muscle contraction.

Neutral endopeptidase modulates endothelin-1-induced airway smooth muscle contraction in guinea-pig trachea

1992 ◽  
Vol 39 (2-3) ◽  
pp. 137-145 ◽  
Author(s):  
Giuseppe U. Di Maria ◽  
Michio Katayama ◽  
D.Benjamin Borson ◽  
Jay A. Nadel
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Muscle Contraction ◽  
Airway Smooth Muscle ◽  
Neutral Endopeptidase ◽  
Smooth Muscle Contraction ◽  
Endothelin 1

Lower airway smooth muscle contraction induced by (red tide) toxin

1987 ◽  
Vol 79 (6) ◽  
pp. 899-908 ◽  
Author(s):  
T SHIMODA ◽  
J KRZANOWSKI ◽  
R LOCKEY ◽  
D MARTIN ◽  
M PEREZCRUET ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Airway Smooth Muscle ◽  
Red Tide ◽  
Smooth Muscle Contraction ◽  
Lower Airway

Contraction of guinea pig ileal smooth muscle by acetyl glyceryl ether phosphorylcholine

1981 ◽  
Vol 241 (3) ◽  
pp. C130-C133 ◽  
Author(s):  
S. R. Findlay ◽  
L. M. Lichtenstein ◽  
D. J. Hanahan ◽  
R. N. Pinckard
Keyword(s):  
Biological Activity ◽  
Smooth Muscle ◽  
Guinea Pig ◽  
Muscle Contraction ◽  
Glyceryl Ether ◽  
Platelet Activating Factor ◽  
Smooth Muscle Contraction

Acetyl glyceryl ether phosphorylcholine (AGEPC) is a chemical that has the biological activity of what was formerly termed platelet-activating factor. We report here that synthetic AGEPC induces the contraction of guinea pig ileal smooth muscle. Antagonists of histamine, acetylcholine, and slow-reacting substances (SRS) do not block AGEPC-induced contraction. These responses were long lasting, resistant to washing, and displayed complete agonist specific desensitization. Histamine- and SRS-induced contractions were unaffected by AGEPC. These studies show that AGEPC has the potential to produce a component of anaphylactically induced smooth muscle contraction.

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