scholarly journals Regulation of NADPH‐cytochrome P450 oxidoreductase by glucocorticoids in rat liver

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Alex Vonk ◽  
David S Riddick
Keyword(s):  
Cytochrome P450 ◽  
Rat Liver ◽  
P450 Oxidoreductase ◽  
Nadph Cytochrome ◽  
Cytochrome P450 Oxidoreductase

Mechanisms of NADPH – cytochrome P450 oxidoreductase induction by dexamethasone in the H4IIE rat hepatoma cell line

2020 ◽  
Vol 98 (5) ◽  
pp. 267-274
Author(s):  
David S. Riddick ◽  
Anne K. Mullen Grey
Keyword(s):  
Cytochrome P450 ◽  
Cell Line ◽  
Rat Liver ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
P450 Oxidoreductase ◽  
Nadph Cytochrome ◽  
Cytochrome P450 Oxidoreductase ◽  
Rat Hepatoma Cell Line ◽  
Rat Hepatoma

Expression of NADPH – cytochrome P450 oxidoreductase (POR), electron donor for microsomal P450s, is induced in rat liver by dexamethasone (DEX), an activator of the glucocorticoid receptor (GR) and the pregnane X receptor (PXR). DEX induction of POR in rat liver is primarily PXR-mediated, although GR may contribute to mRNA effects. We examined the role of GR and PXR in the DEX induction of POR mRNA and protein in the H4IIE rat hepatoma cell line. The DEX EC50 for a PXR target, CYP3A23, exceeded that for the GR targets tyrosine aminotransferase and PXR as well as POR itself. POR protein levels were induced 3- and 4-fold, respectively, by DEX concentrations activating GR selectively (100 nM) or both GR and PXR (10 μM). POR was induced by triamcinolone acetonide, a selective GR agonist, but not pregnenolone-16α-carbonitrile, a selective PXR agonist. POR induction was blocked by the GR antagonist RU486 but minimally influenced by the PXR antagonist FLB-12. The half-life for POR mRNA was prolonged by DEX at both 100 nM and 10 μM. GR is more important in DEX-induced POR expression in H4IIE cells compared to rat liver in vivo, calling into question the suitability of this cell model for mechanistic studies.

Stable Expression of Human Cytochrome P450 3A4 in Conjunction with Human NADPH-Cytochrome P450 Oxidoreductase in V79 Chinese Hamster Cells

1996 ◽  
Vol 332 (2) ◽  
pp. 295-304 ◽  
Author(s):  
Anneliese Schneider ◽  
Wolfgang A. Schmalix ◽  
Vasanthi Siruguri ◽  
Els M. de Groene ◽  
G.Jean Horbach ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Chinese Hamster ◽  
Stable Expression ◽  
Cytochrome P450 3A4 ◽  
Chinese Hamster Cells ◽  
P450 Oxidoreductase ◽  
Nadph Cytochrome ◽  
Human Cytochrome ◽  
Cytochrome P450 Oxidoreductase

Isolation of a New Mouse cDNA Clone: Hybrid Form of Cytochrome P450 2b10 and NADPH-Cytochrome P450 Oxidoreductase

1996 ◽  
Vol 226 (3) ◽  
pp. 900-905 ◽  
Author(s):  
Marie Damon ◽  
Alain Fautrel ◽  
Nathalie Marc ◽  
André Guillouzo ◽  
Laurent Corcos
Keyword(s):  
Cytochrome P450 ◽  
Cdna Clone ◽  
Hybrid Form ◽  
P450 Oxidoreductase ◽  
Nadph Cytochrome ◽  
Mouse Cdna ◽  
Cytochrome P450 Oxidoreductase
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