scholarly journals Identification and molecular characterization of 5′‐regulatory region of the human SLC52A1 (rft‐1) promoter (896.4)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Subrata Sabui ◽  
Hamid Said
Keyword(s):  
Molecular Characterization ◽  
Regulatory Region

scholarly journals Functional and molecular characterization of the transcriptional regulatory region of the proacrosin gene.

1994 ◽  
Vol 269 (51) ◽  
pp. 32181-32186
Author(s):  
K Nayernia ◽  
S Nieter ◽  
H Kremling ◽  
H Oberwinkler ◽  
W Engel
Keyword(s):  
Molecular Characterization ◽  
Regulatory Region ◽  
Transcriptional Regulatory ◽  
Transcriptional Regulatory Region

Molecular characterization of the 5′ regulatory region of the human sodium-dependent multivitamin transporter, SMVT

2001 ◽  
Vol 120 (5) ◽  
pp. A678
Author(s):  
Nabendu S. Chatterjee ◽  
Sanjit Dey ◽  
Stanley A. Rubin ◽  
Hamid M. Said
Keyword(s):  
Molecular Characterization ◽  
Regulatory Region ◽  
Sodium Dependent

In vivo expression and molecular characterization of the porcine slow-myosin heavy chain

1993 ◽  
Vol 106 (1) ◽  
pp. 331-341 ◽  
Author(s):  
K.C. Chang ◽  
K. Fernandes ◽  
G. Goldspink
Keyword(s):  
Myosin Heavy Chain ◽  
Molecular Characterization ◽  
Heavy Chain ◽  
Regulatory Region ◽  
Regulatory Elements ◽  
Slow Myosin ◽  
Slow Myosin Heavy Chain ◽  
In Vivo Expression

We report on the molecular characterization of the porcine slow-myosin heavy chain (HC) beta gene and the isolation of its 5′ end cDNA. In vivo expression study, by in situ hybridization and histochemistry, revealed a highly regular rosette pattern of fiber arrangement, with a slow fiber occupying the central core, in all the skeletal muscles examined. This feature can be advantageous in the distinction of primary and secondary fibers in myogenic lineage studies. In the neonatal heart, beta isoform expression is diffuse, with higher expression occurring in the ventricle than in the atrium. Transient transfection assays showed the porcine promoter functions in a muscle- and differentiation stage-specific manner. In the 5′ regulatory region are several putative positive and negative regulatory elements, including a positive and a negative element in close proximity to each other in intron 1.

Genetic and molecular characterization of a noveliab-8regulatory domain in theAbdominal-Bgene ofDrosophila melanogaster

Development ◽  
2002 ◽  
Vol 129 (22) ◽  
pp. 5195-5204
Author(s):  
Beatriz Estrada ◽  
Fernando Casares ◽  
Ana Busturia ◽  
Ernesto Sánchez-Herrero
Keyword(s):  
Molecular Characterization ◽  
Hox Genes ◽  
Regulatory Region ◽  
Transcription Unit ◽  
Bithorax Complex ◽  
Hox Gene ◽  
Complex Regulation ◽  
Temporal And Spatial ◽  
Derivatives Of

Homeotic (or Hox) genes are key determinants in specifying the anteroposterior axis of most animals. The temporal and spatial expression of these genes requires the presence of large and complex cis-regulatory regions. The Abdominal-B Hox gene of the bithorax complex ofDrosophila is regulated by several infraabdominal domains,which determine Abdominal-B expression in abdominal segments A5 to A9(parasegments 10 to 14). Some of the infraabdominal domains have been characterized, including an infraabdominal-8 domain, which has been located 3′ to the Abdominal-B transcription unit. We have analyzed the expression and mutant phenotype of a P-lacZ element inserted close to the Abdominal-B m origin of transcription and of derivatives of this transposon. Some of these derivatives represent a particular class of mutations in the bithorax complex, because they transform the eighth and ninth abdominal segments without affecting more anterior metameres. The analysis of these mutations and of transformants carrying sequences upstream the Abdominal-B m transcription unit has allowed us to define a new infraabdominal-8 regulatory region, located 5′ to the Abdominal-B transcription unit, and has helped to characterize better the complex regulation of the Abdominal-Bgene.

scholarly journals Functional and molecular characterization of the transcriptional regulatory region of Tcp-10bt, a testes-expressed gene from the t complex responder locus

Development ◽  
1993 ◽  
Vol 117 (1) ◽  
pp. 89-95 ◽  
Author(s):  
U.K. Ewulonu ◽  
T.J. Buratynski ◽  
J.C. Schimenti
Keyword(s):  
Germ Cell ◽  
Molecular Characterization ◽  
Regulatory Region ◽  
Transmission Ratio Distortion ◽  
Cell Protein ◽  
Specific Expression ◽  
T Complex ◽  
Transcriptional Regulatory ◽  
Ratio Distortion

Mouse t haplotypes contain several mutant alleles that disrupt spermatogenesis. Their phenotypes include sterility, reduced fertility and transmission ratio distortion (TRD). The substantial genetic analyses of these mutant alleles, coupled with intensive physical characterization of the t complex, provides a fertile ground for identifying and understanding genes essential to male gametogenesis. The t complex responder (Tcr) locus plays a central role in this process, interacting with other t haplotype-encoded genes to mediate TRD. A candidate responder gene, Tcp-10bt, has been cloned and subjected to molecular characterization. Here, we define the transcriptional regulatory regions of this gene in transgenic mice. A 1.6 kb (but not 0.6 kb) DNA fragment upstream of the transcription start site contains all the regulatory signals for appropriate temporal and germ cell-specific expression of this gene. Two smaller fragments within this region bound specifically to nuclear factor(s) from germ cell protein extracts in gel shift assays. This work is a step towards understanding the mechanism of Tcp-10bt regulated expression and may ultimately help reveal a common regulatory pathway shared by other similarly expressed spermatogenic genes.

1446: Molecular Characterization of Cyclooxygenase-2 in a Stimulated Prostate Stroma Cells

2006 ◽  
Vol 175 (4S) ◽  
pp. 467-467
Author(s):  
Victor K. Lin ◽  
Shih-Ya Wang ◽  
Claus G. Roehrbom
Keyword(s):  
Molecular Characterization ◽  
Cyclooxygenase 2 ◽  
Prostate Stroma
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